Transcriptional regulation of the fatty acid binding protein 2 (FABP2) gene by the hepatic nuclear factor 1 alpha (HNF-1α)

Klapper, Maja; Böhme, Mike; Nitz, Inke; Döring, Frank

doi:10.1016/j.gene.2008.02.025

Cited by 5 publications

(3 citation statements)

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“…FABP2 predominantly expressed in enterocytes belongs to FABPs, which is cytoplasmic proteins touched upon intracellular fatty acid transport and metabolism [ 24 , 25 ]. Previous studies have identified that FABP2 plays a key role in the absorption and intracellular transport of dietary long-chain fatty acids [ 26 , 27 ], and the explanation of observed fatty acids alterations in Major Depressive Disorder [ 28 ]. Further, FABP2 Ala54Thr polymorphism, a mutant phenotype, is closely related to insulin resistance and abnormal lipid metabolism, which are the main risk factors for cardiovascular disease [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of hepatic transcriptome demonstrates altered lipid metabolism following Lactobacillus johnsonii BS15 prevention in chickens with subclinical necrotic enteritis

et al. 2018

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BackgroundSubclinical necrotic enteritis (SNE) widely outbreaks in chickens which inflicted growth-slowing, causing enormous social and economic burdens. To better understand the molecular underpinnings of SNE on lipid metabolism and explore novel preventative strategies against SNE, we studied the regulatory mechanism of a potential probiotic, Lactobacillus johnsonii BS15 on the lipid metabolism pathways involved in chickens with SNE.MethodsOne hundred eighty one-day-old chickens were randomly divided into three groups and arranged with basal diet (control and SNE group). Added with BS15 (1 × 106 cfu/g) or Man Rogosa Sharpe (MRS) liquid medium for 28 days. The hepatic gene expression of each group was then measured using high-throughput analysis methods (RNA-Seq). Quantitative real-time PCR (qRT-PCR) was used to detect the expression changes of the related genes.ResultsThe results showed that there are eleven lipid metabolic pathways were found during the prevention of BS15 treatment in SNE chickens by RNA-Seq, including the peroxisome proliferator-activated receptor (PPAR) signaling pathway and arachidonic acid metabolism. BS15 notably facilitated the expressions of fatty acid binding protein 2 (FABP2), acyl-CoA synthetase bubblegum family member 1 (ACSBG1), perilipin 1 (PLIN1) and perilipin 2 (PLIN2), which were involved in PPAR signaling pathway of SNE chickens. Besides, suppression of phospholipase A2 group IVA (PLA2G4A) in arachidonic acid metabolism was observed in SNE chickens after BS15 prevention. The expression patterns of FABP2, ACSBG1, PLIN1, PLIN2 and PLA24G in qRT-PCR validation were consistent with RNA-Seq results.ConclusionsThese findings indicate that SNE may affect the hepatic lipid metabolism of chickens. Meanwhile, BS15 pretreatment may provide a prospective natural prophylaxis strategy against SNE through improving the PPAR signaling pathway and arachidonic acid metabolism.Electronic supplementary materialThe online version of this article (10.1186/s12944-018-0741-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Analysis of hepatic transcriptome demonstrates altered lipid metabolism following Lactobacillus johnsonii BS15 prevention in chickens with subclinical necrotic enteritis

et al. 2018

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“…Amplification was done using a Touch‐up PCR increasing annealing temperature three times by 2°C, starting 2°C under the specific primer Tm. Human hepatocyte nuclear factor (HNF)‐1α and HNF‐4α expression clones are described in Klapper et al [22, 23]. Open reading frames (ORFs) of ACBP splice variants 1A(2), 1A(1)‐G, 1G and 1D were cloned into the expression vector pcDest40 to generate C‐terminal fusions with V5 tag.…”

Section: Methodsmentioning

confidence: 99%

Specific regulation of low-abundance transcript variants encoding human Acyl-CoA binding protein (ACBP) isoforms

Nitz¹,

Kruse²,

Klapper³

et al. 2011

Journal of Cellular and Molecular Medicine

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Despite intensive efforts on annotation of eukaryotic transcriptoms, little is known about the regulation of low-abundance transcripts. To address this question, we analysed the regulation of novel low-abundance transcript variants of human acyl-CoA binding protein (ACBP), an important multifunctional housekeeping protein, which we have identified by screening of human expressed sequence tags in combination with ab initio gene prediction. By using RT-, real-time RT- and rapid amplification of cDNA ends-PCR in five human tissues, we find these transcripts, which are generated by a consequent use of alternative promoters and alternate first or first two exons, to be authentic ones. They show a tissue-specific distribution and intrinsic responsiveness to glucose and insulin. Promoter analyses of the corresponding transcripts revealed a differential regulation mediated by sterol regulatory element-binding protein-2, hepatocyte nuclear factor-4α and nuclear factor κB (NF-κB), central transcription factors of fat and glucose metabolism and inflammation. Subcellular localization studies of deduced isoforms in liver HepG2 cells showed that they are distributed in different compartments. By demonstrating that ACBP is a target of NF-κB, our findings link fatty acid metabolism with inflammation. Furthermore, our findings show that low-abundance transcripts are regulated in a similar mode than their high-abundance counterparts.

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“…Due to their physiological properties, fatty acid-binding proteins (FABPs) loci are candidate genes for IMF content. The cellular functions of FABPs are very versatile (Klapper et al, 2008). FABPs influence metabolic pathways by binding and intracellular transport of fatty acids and other ligands (Haunerland and Spener, 2004;Prinsen et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Association of A-FABP gene polymorphism in intron 1 with meat quality traits in Junmu No. 1 white swine

Gao

Zhang

et al. 2011

Gene

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Transcriptional regulation of the fatty acid binding protein 2 (FABP2) gene by the hepatic nuclear factor 1 alpha (HNF-1α)

Cited by 5 publications

References 44 publications

Analysis of hepatic transcriptome demonstrates altered lipid metabolism following Lactobacillus johnsonii BS15 prevention in chickens with subclinical necrotic enteritis

Analysis of hepatic transcriptome demonstrates altered lipid metabolism following Lactobacillus johnsonii BS15 prevention in chickens with subclinical necrotic enteritis

Specific regulation of low-abundance transcript variants encoding human Acyl-CoA binding protein (ACBP) isoforms

Association of A-FABP gene polymorphism in intron 1 with meat quality traits in Junmu No. 1 white swine

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