Transcriptional regulation of the intercellular adhesion molecule-1 gene by proinflammatory cytokines in human astrocytes

Lee, Sung Joong; Park, Joo Young; Hou, Jinzhao; Benveniste, Etty N.

doi:10.1002/(sici)1098-1136(19990101)25:1<21::aid-glia3>3.0.co;2-r

Cited by 35 publications

(18 citation statements)

References 53 publications

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“…Interestingly, gene expression of VCAM-1 detected much higher and earlier than ICAM-1 gene expression. Since initial identification as an endothelial cell surface glycoprotein, previous studies reported ICAM-1 and VCAM-1 expression in nonvascular CNS cells, such as astrocytes, neuroblastoma and glioblastoma [1,2,12,[14][15][16][17][18]. The interaction of astrocytes with endothelial cells in the formation of BBB implies a critical role in the neuroinflammation response [1].…”

Section: Discussionmentioning

confidence: 99%

“…AP-1 and NF-κB are well known as important TFs in the expression of several inflammatory-related genes in glial cells. The STAT-1 binding site (GAS) located in the promoter region of ICAM-1 gene is required for induction by cytokines [11][12][13]. In this study, we examined which TFs are involved in CM-stimulated astrocytes using several cis-acting element luciferase vectors.…”

Section: Discussionmentioning

confidence: 99%

“…The induction of ICAM-1 depends on the transcription factors (TFs), such as AP-1 and NF-κB, which are activated by proinflamatory cytokines [11][12][13]. However, the involvement of LacCer and TFs, such as AP-1, NF-κB and STATs on ICAM-1 and VCAM-1 expression in astrocytes is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Lactosylceramide Mediates the Expression of Adhesion Molecules in TNF-α and IFNγ-stimulated Primary Cultured Astrocytes

Lee

Kim

Park

et al. 2011

Korean J Physiol Pharmacol

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lactosylceramide Mediates the Expression of Adhesion Molecules in TNF-α and IFNγ-stimulated Primary Cultured Astrocytes

Lee

Kim

Park

et al. 2011

Korean J Physiol Pharmacol

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“…Total cellular RNA was isolated from confluent monolayers of EOC13 cells or astrocytes that were incubated with cytokines or anti-Fas Ab, respectively, as previously described (31). Briefly, cells were washed once in PBS and lysed directly in the culture dish.…”

Section: Rna Isolation and Rnase Protection Assay (Rpa)mentioning

confidence: 99%

“…RNA was extracted with guanidinium isothiocyanate and phenol, and precipitated with ethanol. Ten micrograms of total cellular RNA was analyzed by RPA using an RPA kit (Ambion, Austin, TX) as previously described (31). Total RNA was hybridized with both mouse Fas and GAPDH riboprobes (2.5 ϫ 10 5 cpm) at 42°C overnight in 20 l of 40 mM PIPES (pH 6.4), 80% deionized formamide, 400 mM NaOAc, and 1 mM EDTA.…”

Section: Rna Isolation and Rnase Protection Assay (Rpa)mentioning

confidence: 99%

Differential Regulation and Function of Fas Expression on Glial Cells

Lee¹,

Zhou

Choi³

et al. 2000

The Journal of Immunology

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121

104

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Fas/Apo-1 is a member of the TNF receptor superfamily that signals apoptotic cell death in susceptible target cells. Fas or Fas ligand (FasL)-deficient mice are relatively resistant to the induction of experimental allergic encephalomyelitis, implying the involvement of Fas/FasL in this disease process. We have examined the regulation and function of Fas expression in glial cells (astrocytes and microglia). Fas is constitutively expressed by primary murine microglia at a low level and significantly up-regulated by TNF-α or IFN-γ stimulation. Primary astrocytes express high constitutive levels of Fas, which are not further affected by cytokine treatment. In microglia, Fas expression is regulated at the level of mRNA expression; TNF-α and IFN-γ induced Fas mRNA by ∼20-fold. STAT-1α and NF-κB activation are involved in IFN-γ- or TNF-α-mediated Fas up-regulation in microglia, respectively. The cytokine TGF-β inhibits basal expression of Fas as well as cytokine-mediated Fas expression by microglia. Upon incubation of microglial cells with FasL-expressing cells, ∼20% of cells underwent Fas-mediated cell death, which increased to ∼60% when cells were pretreated with either TNF-α or IFN-γ. TGF-β treatment inhibited Fas-mediated cell death of TNF-α- or IFN-γ-stimulated microglial cells. In contrast, astrocytes are resistant to Fas-mediated cell death, however, ligation of Fas induces expression of the chemokines macrophage inflammatory protein-1β (MIP-1β), MIP-1α, and MIP-2. These data demonstrate that Fas transmits different signals in the two glial cell populations: a cytotoxic signal in microglia and an inflammatory signal in the astrocyte.

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Immune function of astrocytes

Dong

Benveniste

2001

Glia

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1,164

877

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Astrocytes are the major glial cell within the central nervous system (CNS) and have a number of important physiological properties related to CNS homeostasis. The aspect of astrocyte biology addressed in this review article is the astrocyte as an immunocompetent cell within the brain. The capacity of astrocytes to express class II major histocompatibility complex (MHC) antigens and costimulatory molecules (B7 and CD40) that are critical for antigen presentation and T-cell activation are discussed. The functional role of astrocytes as immune effector cells and how this may influence aspects of inflammation and immune reactivity within the brain follows, emphasizing the involvement of astrocytes in promoting Th2 responses. The ability of astrocytes to produce a wide array of chemokines and cytokines is discussed, with an emphasis on the immunological properties of these mediators. The significance of astrocytic antigen presentation and chemokine/cytokine production to neurological diseases with an immunological component is described.

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Transcriptional regulation of the intercellular adhesion molecule-1 gene by proinflammatory cytokines in human astrocytes

Cited by 35 publications

References 53 publications

Lactosylceramide Mediates the Expression of Adhesion Molecules in TNF-α and IFNγ-stimulated Primary Cultured Astrocytes

Lactosylceramide Mediates the Expression of Adhesion Molecules in TNF-α and IFNγ-stimulated Primary Cultured Astrocytes

Differential Regulation and Function of Fas Expression on Glial Cells

Immune function of astrocytes

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