Transcriptional Regulation of Yeast Oxidative Phosphorylation Hypoxic Genes by Oxidative Stress

Liu, Jingjing; Barrientos, Antoni

doi:10.1089/ars.2012.4589

Cited by 36 publications

(28 citation statements)

References 44 publications

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“…Induction of ROX1 expression was, however, shown to be Yap1-independent. The discrepancy between the data of Liu and Barrientos (51), and our own data are likely due to differences in the quantitative analysis of ROX1 transcripts (relative expression versus fold change). Here, we clearly show that Yap1 up-regulates ROX1 gene (Fig.…”

Section: Discussioncontrasting

confidence: 91%

Repression of the Low Affinity Iron Transporter Gene FET4

Caetano

Menezes

Amaral

et al. 2015

Journal of Biological Chemistry

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Background: Yap1 regulates cadmium accumulation in the vacuole and mitigates cadmium-induced ROS. Results: Yap1 induces the gene of the hypoxic repressor Rox1 that in turn represses FET4, avoiding cadmium uptake. Conclusion: Repression of FET4, via Rox1, is a novel line of defense mediated by Yap1 against cadmium toxicity. Significance: Evidence of cross-talk between oxidative and hypoxic regulators that results in increased tolerance to metal toxicity.

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Section: Discussioncontrasting

confidence: 91%

Repression of the Low Affinity Iron Transporter Gene FET4

Caetano

Menezes

Amaral

et al. 2015

Journal of Biological Chemistry

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“…During the evolution of eukaryotic COX, several additional subunits encoded in the nuclear genome were progressively incorporated. One of the earliest nuclear addition events involved the emergence of subunit Cox5 (yeast)/COX4 (mammals), which exist in 2 isoforms whose expression is inversely regulated transcriptionally by oxygen tension and oxidative stress (55, 60–62). This subunit is highly conserved across phyla and provides a substrate for regulation of COX function and eukaryotic respiratory metabolism (63).…”

Section: Discussionmentioning

confidence: 99%

A mitochondrial CO₂‐adenylyl cyclase‐cAMP signalosome controls yeast normoxic cytochromecoxidase activity

Hess

Liu²,

Manfredi

et al. 2014

FASEB j.

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Mitochondria, the major source of cellular energy in the form of ATP, respond to changes in substrate availability and bioenergetic demands by employing rapid, short-term, metabolic adaptation mechanisms, such as phosphorylation-dependent protein regulation. In mammalian cells, an intramitochondrial CO2-adenylyl cyclase (AC)-cyclic AMP (cAMP)-protein kinase A (PKA) pathway regulates aerobic energy production. One target of this pathway involves phosphorylation of cytochrome c oxidase (COX) subunit 4-isoform 1 (COX4i1), which modulates COX allosteric regulation by ATP. However, the role of the CO2-sAC-cAMP-PKA signalosome in regulating COX activity and mitochondrial metabolism and its evolutionary conservation remain to be fully established. We show that in Saccharomyces cerevisiae, normoxic COX activity measured in the presence of ATP is 55% lower than in the presence of ADP. Moreover, the adenylyl cyclase Cyr1 activity is present in mitochondria, and it contributes to the ATP-mediated regulation of COX through the normoxic subunit Cox5a, homologue of human COX4i1, in a bicarbonate-sensitive manner. Furthermore, we have identified 2 phosphorylation targets in Cox5a (T65 and S43) that modulate its allosteric regulation by ATP. These residues are not conserved in the Cox5b-containing hypoxic enzyme, which is not regulated by ATP. We conclude that across evolution, a CO2-sAC-cAMP-PKA axis regulates normoxic COX activity.

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“…Cys3 and Cys4 are two enzymes of the sulfur amino acid and glutathione (GSH) biosynthesis pathways, with the first one being the rate-limiting enzyme of GSH biosynthesis, and have been identified through a proteomic approach to be induced by cadmium stress (Vido et al, 2001). It is also not surprising that deletion of ROX1 renders yeast cells sensitive to cadmium, as cadmium ions cause yeast cells an oxidative stress, and Rox1 is a heme-dependent repressor that mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7 (Lee et al, 2011;Liu & Barrientos, 2013). The CWI pathway is involved in the response of yeast cells to H 2 O 2 stress (Lee et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast

et al. 2014

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Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive to a sublethal concentration of cadmium, and 64 mutants show elevated intracellular cadmium concentrations upon exposure to cadmium. Hog1 is phosphorylated, reaching a peak 30 min after the cadmium treatment. Both Sln1 and Sho1 upstream branches are involved in the cadmium-induced activation of high osmolarity glycerol (HOG) pathway. Cadmium-induced HOG activation is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in the Sln1 branch.

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Transcriptional Regulation of Yeast Oxidative Phosphorylation Hypoxic Genes by Oxidative Stress

Cited by 36 publications

References 44 publications

Repression of the Low Affinity Iron Transporter Gene FET4

Repression of the Low Affinity Iron Transporter Gene FET4

A mitochondrial CO₂‐adenylyl cyclase‐cAMP signalosome controls yeast normoxic cytochromecoxidase activity

Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast

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Transcriptional Regulation of Yeast Oxidative Phosphorylation Hypoxic Genes by Oxidative Stress

Cited by 36 publications

References 44 publications

Repression of the Low Affinity Iron Transporter Gene FET4

Repression of the Low Affinity Iron Transporter Gene FET4

A mitochondrial CO2‐adenylyl cyclase‐cAMP signalosome controls yeast normoxic cytochromecoxidase activity

Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast

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A mitochondrial CO₂‐adenylyl cyclase‐cAMP signalosome controls yeast normoxic cytochromecoxidase activity