Transcriptional reprogramming during recovery from drought stress in <i>Eucalyptus grandis</i>

Teshome, Demissew Tesfaye; Zharare, Godfrey Elijah; Ployet, Raphaël; Naidoo, Sanushka

doi:10.1093/treephys/tpad022

Cited by 6 publications

(3 citation statements)

References 108 publications

(111 reference statements)

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“…under abiotic stress). Recently, RNAseq analysis of the E. grandis stem transcriptome has revealed that genes associated with growth and metabolism were downregulated during drought, while responses to reactive oxygen species (ROS) and phytohormone signaling pathways were upregulated ( Teshome et al. 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Keret,

Drew,

Hills

2024

Tree Physiology

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Future climatic scenarios forecast increasingly frequent droughts that will pose substantial consequences on tree mortality. In light of this, drought-tolerant eucalypts have been propagated, however, the severity of these conditions will invoke adaptive responses, impacting the commercially valuable wood properties. To determine what mechanisms govern the wood anatomical adaptive response, highly controlled drought experiments were conducted in E. grandis, with the tree physiology and transcriptome closely monitored. In response to water deficit, E. grandis displays an isohydric stomatal response to conserve water and enable stem growth to continue, albeit at a reduced rate. Maintaining gaseous exchange is likely a critical short-term response that drives the formation of hydraulically-safer xylem. For instance, the development of significantly smaller fibers and vessels was found to increase cellular density, thereby promoting drought tolerance through improved functional redundancy, as well as implosion and cavitation resistance. The transcriptome was explored to identify the molecular mechanisms responsible for controlling xylem cell size during prolonged water deficit. Downregulation of genes associated with cell wall remodelling and the biosynthesis of cellulose, hemicellulose, and pectin appeared to coincide with a reduction in cellular enlargement during drought. Furthermore, transcript levels of NAC and MYB transcription factors, vital for cell wall component biosynthesis, were reduced, while those linked to lignification increased. The upregulation of EgCAD and various peroxidases under water deficit did not correlate with an increased lignin composition. However, with the elevated cellular density, a higher lignin content per xylem cross-sectional area was observed, potentially enhancing hydraulic safety. These results support the requirement for higher density, drought-adapted wood as a long-term adaptive response in E. grandis, which is largely influenced by the isohydric stomatal response coupled with cellular expansion-related molecular processes.

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Section: Introductionmentioning

confidence: 99%

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Keret,

Drew,

Hills

2024

Tree Physiology

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Section: Resultsmentioning

confidence: 99%

“…To uncover potential homologs of AQP genes associated with responses to drought stress, we conducted an analysis of the RNAseq dataset for Eucalyptus grandis exposed to drought stress (DS) and subsequent rewatering (RW) conditions as described in Teshome et al (2023). We employed a significance threshold with an adjusted p-value of less than 0.05 and a log fold change (logFC) greater than 2 to identify differentially expressed genes (DEGs).…”

Section: Expression Profile Of Egaqps Under Drought Stress and Rewate...mentioning

confidence: 99%

Comprehensive analysis of the Aquaporin genes inEucalyptus grandissuggests potential targets for drought stress tolerance

Seidel,

Claudino,

Sperotto

et al. 2023

Preprint

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This study delves into the comprehensive analysis ofAQPgenes inEucalyptus grandis, providing insights into their genomic abundance, diversification, expression patterns across tissues, and responses to drought stress. We identified 53AQPgenes in theEucalyptus grandisgenome, categorized into four subfamilies:AQP-NIP,AQP-SIP,AQP-PIP, andAQP-TIP. This abundance ofAQPgenes is a reflection of gene duplications, both tandem and whole-genome, which have shaped their expansion. The chromosomal distribution of these genes reveals their widespread presence across the genome, with some subfamilies exhibiting more tandem duplications, suggesting distinct roles and evolutionary pressures. Sequence analysis uncovered characteristic motifs specific to different AQP subfamilies, demonstrating the diversification of protein and targeting. The expression profiles ofAQPgenes in various tissues in bothArabidopsis thalianaandEucalyptus grandisshowcased variations, with root tissues showing higher expression levels. Notably,AQP-PIPgenes consistently exhibited robust expression across tissues, highlighting their importance in maintaining water regulation within plants. Furthermore, the study investigated the response ofAQPgenes to drought stress and rehydration, revealing differential expression patterns.EgAQP-NIPandEgAQP-TIPgenes were up-regulated during drought stress, emphasizing their role in osmotic equilibrium and water transport. Conversely,EgAQP-PIPgenes showed down-regulation during drought stress but were up-regulated upon rehydration, indicating their involvement in water movement across cell membranes. Overall, this research contributes to our understanding ofAQPgenes inEucalyptus grandis, shedding light on their genomic evolution, expression patterns, and responses to environmental challenges, particularly drought stress. This information can be valuable for future studies aimed at enhancing the drought resilience of woody perennial plants likeEucalyptus grandis.

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Comprehensive Analysis of the Aquaporin Genes in Eucalyptus grandis Suggests Potential Targets for Drought Stress Tolerance

Seidel,

Claudino,

Sperotto

et al. 2024

Tropical Plant Biol.

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Transcriptional reprogramming during recovery from drought stress in Eucalyptus grandis

Cited by 6 publications

References 108 publications

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Comprehensive analysis of the Aquaporin genes inEucalyptus grandissuggests potential targets for drought stress tolerance

Comprehensive Analysis of the Aquaporin Genes in Eucalyptus grandis Suggests Potential Targets for Drought Stress Tolerance

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