Transcriptional reprogramming of somatic cell nuclei during preimplantation development of cloned bovine embryos

Beyhan, Zeki; Ross, Pablo J.; Iager, Amy E.; Kocabas, Arif; Cunniff, Kerrianne; Rosa, Guilherme J. M.; Cibelli, José B.

doi:10.1016/j.ydbio.2007.01.041

Cited by 51 publications

(62 citation statements)

References 49 publications

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“…This led us to speculate that they were a consequence of the inhibition of protein synthesis (CHX) or protein phosphorylation (DMAP), but not because of the abnormal [Ca 2C ] i regimen. On the other hand, in agreement with our previous report (Beyhan et al 2007), U2AF1L2, a gene involved in RNA splicing, was only detected in IVF embryos, indicating that reprogramming of this locus failed in SCNT embryos irrespective of the activation protocol. Although the role of U2AF1L2 in embryonic development has not been investigated, the intrinsic capacity of this gene to affect several cellular processes could lead to potentially serious alterations in the ability of cloned embryos to develop normally.…”

Section: Activation Of Cloned Embryos By Plczsupporting

confidence: 92%

“…POU5F1, NANOG, and SOX2 have been characterized as important for pluripotency and ICM formation, while the transcription factor CDX2 and the FGF receptor type 2 (FGFR2) have been shown to be expressed specifically in the TE of mouse embryos. We also determined the expression of TRYP8, a gene expressed by somatic cells but not during pre-implantation development of fertilized embryos, and of U2AF1L2, a gene that in a previous study (Beyhan et al 2007) we found discriminated between IVF and SCNT embryos. Blastocyst gene expression was normalized to the exogenous control and then to the total cell number in the embryo, as determined just ] i oscillations in SCNT embryos activated using PLCZ cRNA injection.…”

Section: Embryonic Development Of Cloned Embryos Activated By Plcz Crmentioning

confidence: 99%

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Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection

Ross¹,

Rodríguez²,

Iager³

et al. 2009

Reproduction

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The production of cloned animals by the transfer of a differentiated somatic cell into an enucleated oocyte circumvents fertilization. During fertilization, the sperm delivers a sperm-specific phospholipase C (PLCZ) that is responsible for triggering Ca 2C oscillations and oocyte activation. During bovine somatic cell nuclear transfer (SCNT), oocyte activation is artificially achieved by combined chemical treatments that induce a monotonic rise in intracellular Ca 2C and inhibit either phosphorylation or protein synthesis. In this study, we tested the hypothesis that activation of bovine nuclear transfer embryos by PLCZ improves nuclear reprogramming. Injection of PLCZ cRNA into bovine SCNTunits induced Ca 2C oscillations similar to those observed after fertilization and supported high rates of blastocyst development similar to that seen in embryos produced by IVF. Furthermore, gene expression analysis at the eight-cell and blastocyst stages revealed a similar expression pattern for a number of genes in both groups of embryos. Lastly, levels of trimethylated lysine 27 at histone H3 in blastocysts were higher in bovine nuclear transfer embryos activated using cycloheximide and 6-dimethylaminopurine (DMAP) than in those activated using PLCZ or derived from IVF. These results demonstrate that exogenous PLCZ can be used to activate bovine SCNT-derived embryos and support the hypothesis that a fertilization-like activation response can enhance some aspects of nuclear reprogramming.

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Section: Activation Of Cloned Embryos By Plczsupporting

confidence: 92%

Section: Embryonic Development Of Cloned Embryos Activated By Plcz Crmentioning

confidence: 99%

Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection

Ross¹,

Rodríguez²,

Iager³

et al. 2009

Reproduction

Self Cite

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“…Interestingly, less than twenty genes are typically deregulated in these embryos, and it is important to identify which gene networks are disrupted (Beyhan et al, 2007). A recent microarray study used Trichostatin A to evaluate the effects of assisted epigenetic modification of NT bovine blastocyst transcriptional profiles.…”

Section: Modeling Cellular Epigenetic Status For Nuclear Transfermentioning

confidence: 99%

Achievements and perspectives in cloned and transgenic cattle production by nuclear transfer: influence of cell type, epigenetic status and new technology

Curcio¹,

Bressan²,

Meirelles³

et al. 2017

Anim Reprod

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Genetically modified cattle production is motivated by many factors, including recombinant protein production for therapeutic purposes, disease models and animals presenting improved production traits. Nuclear transfer (NT), combined with efficient cultivation methods, genetic modification and donor cell selection is important for transgenic cattle production. Studies have found that adult cells (such as fibroblasts and cumulus cells, among others) used as nuclear donors achieved results similar to those of fetal cells, with the advantages of easier collection and a known genotype/phenotype. However, no consensus has been reached on the influence of cell type on transgene expression levels and post-reprogramming capacity after nuclear transfer, and these factors appear to be related to epigenetic factors. The development of new strategies, such as chromatin-modifying agents (CMAs), in vitro generation of induced pluripotent cells (iPS cells) and precise genome editing techniques are being explored and may influence nuclear reprogramming success for efficiently producing genetically modified bovine clones.

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“…Large-scale evaluation of the expression of genes [9][10][11][12][13][14] Electron microscopy techniques…”

Section: Conventional and Quantitative Rt-pcrmentioning

confidence: 99%

Immunocytochemistry in Early Mammalian Embryos

Dehghani¹

2012

Applications of Immunocytochemistry

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Transcriptional reprogramming of somatic cell nuclei during preimplantation development of cloned bovine embryos

Cited by 51 publications

References 49 publications

Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection

Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection

Achievements and perspectives in cloned and transgenic cattle production by nuclear transfer: influence of cell type, epigenetic status and new technology

Immunocytochemistry in Early Mammalian Embryos

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