Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Gong, Jing; Tian, Sha; Zhou, Xia; Yang, Huan; Zhu, Yong; Hou, Yong

doi:10.3390/ijms17121838

Cited by 11 publications

(7 citation statements)

References 50 publications

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“…Bivoltine silkworms (Dazao) were provided by the State Key Laboratory of Silkworm Genome Biology. As per the approach used in our previous study [ 13 ], diapause-destined eggs were obtained through the incubation of the parental generation under long-day conditions (18L/6D h) at 25 °C during embryonic development. Samples from diapause eggs were collected at 20 and 48 h oviposition, immediately frozen in liquid nitrogen, and stored at −80 °C until use.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies on silkworm eggs via RNA sequencing showed that the gene expression patterns of hyperoxia- and HCl-treated diapause eggs were similar, which differed from non-treated diapause eggs [ 13 ]. Further physiological research has shown that reactive oxygen species (ROS) levels increase markedly in diapause eggs during this phase, but remain stable in non-diapause eggs, indicating that ROS may play a key role in promoting the onset of diapause [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Early Molecular Events during Onset of Diapause in Silkworm Eggs Revealed by Transcriptome Analysis

Gong

Zheng

Zhao

et al. 2020

IJMS

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Diapause is a form of dormancy, and Bombyx mori silkworm embryos are ideal models for studying diapause in insects. However, molecular events in eggs during the onset of diapause remain unclear. In this study, transcriptome analyses were performed on silkworm diapause eggs via RNA sequencing at 20 and 48 h after oviposition. A total of 6402 differentially expressed genes (DEGs) were detected in diapause eggs at 48 h versus that at 20 h after oviposition. Gene ontology enrichment analysis showed that DEGs in diapause eggs at 48 h versus that at 20 h after oviposition were involved in ribosome-related metabolism and hydrogen transport. Kyoto Encyclopedia of Genes and Genomes analysis revealed several significantly enriched biological pathways, namely the oxidative phosphorylation, Forkhead box protein O3 (FoxO) signaling, ribosome, endoplasmic reticular protein processing, and autophagy pathways. Fifteen DEGs from the FoxO signaling pathway were selected, and their expression profiles were consistent with the transcriptome results from real-time quantitative reverse transcription polymerase chain reaction. Our results can improve understanding of the diapause mechanism in silkworm eggs and identified key pathways for future studies.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Early Molecular Events during Onset of Diapause in Silkworm Eggs Revealed by Transcriptome Analysis

Gong

Zheng

Zhao

et al. 2020

IJMS

Self Cite

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“…6). It was reported that the rate of ribosome synthesis during diapause was lower than that of non-diapause eggs in Bombyx mori, so the upregulation of ribosomal proteins played an important role in blocking diapause [47,48] . In mosquitos, Culex pipiens, the expression of rpS3a was dramatically reduced for a short time in the diapause stage.…”

Section: Comparative Analysis Of Genes Involved In Diapausementioning

confidence: 99%

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Zhang¹,

Du²,

Zhang³

et al. 2020

Preprint

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Background The parasitoid insect Trichogramma dendrolimi can successfully enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have focused mostly on ecological characteristics, so the molecular mechanism of diapause in T. dendrolimi is mostly unknown. In this study, we compared transcriptomes of diapause and non-diapause T. dendrolimi to identify genes involved in the development of diapause. Results Transcriptome sequencing was performed using different samples, including diapause prepupae, pupae after diapause, normal prepupae, and pupae. This initially yielded a total of 87,022 unigenes with an average length of 1,604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7,593 sequences with an average length of 3351 bp. Among the differentially expressed genes, 1,204 were specifically expressed during the diapause stage, and 820 were significantly up-regulated. The result of GO enrichment analysis revealed that binding, oxidation−reduction process, and ribosome biogenesis were significantly affected. Ten genes were selected for validation using a quantitative real-time PCR (qPCR). The changes showed the same trend between the qPCR and RNA-Seq results. Based on previous studies and our data, several genes were identified to be involved in diapause, including UDP-glucuronosyltransferase; Glutathione-S-transferase, ATGs, zinc finger protein genes, cell cycle-related genes, Trehalase, trehalose transporter, hsp68, p53, and DNA damage-regulated gene 1 (pdrg1), and genes related to lipid metabolism were also included. Conclusions In this study, we generated a great amount of transcriptome data from T. dendrolimi, providing a resource for gene function research. The diapause-related genes that we identified establish a valuable basis for future studies on the molecular mechanisms of diapause, not only for T. dendrolimi, but for other species as well.

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“…7). It has been reported that the rate of ribosome synthesis during diapause was lower than that of non-diapause eggs in Bombyx mori, so the upregulation of ribosomal proteins played an important role in blocking diapause [56,57] . In mosquitos, C. pipiens, the expression of ribosomal protein S3a (rpS3a) was dramatically reduced for a short time in diapause stage.…”

Section: Comparative Analysis Of Genes Involved In Diapausementioning

confidence: 99%

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Zhang

Du²,

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The parasitoid wasp Trichogramma dendrolimi can successfully enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi . Previous studies on diapause have focused largely on ecological characteristics, so the molecular mechanism of diapause in T. dendrolimi is mostly unknown. In this study, we compared transcriptomes of diapause and non-diapause T. dendrolimi to identify key genes and pathways involved in the development of diapause. Results: Transcriptome sequencing was performed using different samples, including diapause prepupae, pupae after diapause, normal prepupae, and pupae. Initially, it yielded a total of 87,022 unigenes with an average length of 1,604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7,593 sequences with an average length of 3,351 bp. Among them, 5,702 genes were differentially expressed. The result of Gene Ontology (GO) enrichment analysis revealed that regulation of transcription, DNA-templated, oxidation-reduction process, and signal transduction were significantly affected. Ten genes were selected for validation using a quantitative real-time PCR (qPCR). The changes showed the same trend between the qPCR and RNA-Seq results. Based on our data, several genes were identified to be involved in diapause, including ribosomal proteins, zinc finger proteins, homeobox proteins, forkhead box proteins, UDP-glucuronosyltransferase , Glutathione-S-transferase , p53 , DNA damage-regulated gene 1 ( pdrg1 ), and genes related to lipid metabolism were also included. Conclusions: In this study, we generated a great amount of transcriptome data from T. dendrolimi , providing a resource for gene function research. The diapause-related genes that we identified establish a valuable basis for future studies on the molecular mechanisms of diapause, not only for T. dendrolimi , but for other species as well.

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Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Cited by 11 publications

References 50 publications

Early Molecular Events during Onset of Diapause in Silkworm Eggs Revealed by Transcriptome Analysis

Early Molecular Events during Onset of Diapause in Silkworm Eggs Revealed by Transcriptome Analysis

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

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