Transcriptional Responses in Adult Zebrafish (Danio rerio) Exposed to Propranolol and Metoprolol

Sun, Liwei; Liu, Fang; Chen, Haigang; Wang, Sisi; Xia, Lin; Chi, Jian; Zhu, Qing; Fu, Zhengwei

doi:10.1007/s10646-015-1510-0

Cited by 16 publications

(2 citation statements)

References 36 publications

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“…The presence of β-blockers has been detected in effluents at concentrations with units ranging from of ng/L to µg/L [106,107]. Similar to other pharmaceuticals, their presence in the aquatic ecosystem poses significant harm to organisms such as fish, phytoplankton and daphnia [108][109][110].…”

Section: Anxiolytics Sympatholyticsmentioning

confidence: 99%

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Chmelová,

Ondrejovič,

Miertuš

2024

Life

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This review aims to provide a comprehensive overview of the application of bacterial and fungal laccases for the removal of pharmaceuticals from the environment. Laccases were evaluated for their efficacy in degrading pharmaceutical substances across various categories, including analgesics, antibiotics, antiepileptics, antirheumatic drugs, cytostatics, hormones, anxiolytics, and sympatholytics. The capability of laccases to degrade or biotransform these drugs was found to be dependent on their structural characteristics. The formation of di-, oligo- and polymers of the parent compound has been observed using the laccase mediator system (LMS), which is advantageous in terms of their removal via commonly used processes in wastewater treatment plants (WWTPs). Notably, certain pharmaceuticals such as tetracycline antibiotics or estrogen hormones exhibited degradation or even mineralization when subjected to laccase treatment. Employing enzyme pretreatment mitigated the toxic effects of degradation products compared to the parent drug. However, when utilizing the LMS, careful mediator selection is essential to prevent potential increases in environment toxicity. Laccases demonstrate efficiency in pharmaceutical removal within WWTPs, operating efficiently under WWTP conditions without necessitating isolation.

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Section: Anxiolytics Sympatholyticsmentioning

confidence: 99%

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Chmelová,

Ondrejovič,

Miertuš

2024

Life

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“…In addition, adrb2a is also known as the gene encoding the β-adrenergic receptor in zebrafish, which is localized in the brain and involved in the oxidative stress response. 45 Our previous study also found that AgNP can induce oxidative stress in zebrafish. 17 Lysophosphatidic acid (LPA) was robustly expressed in migratory neurons, which depleted neurons show impaired multipolar-to-bipolar transition.…”

Section: Discussionmentioning

confidence: 89%

Silver Nanoparticles Cause Neural and Vascular Disruption by Affecting Key Neuroactive Ligand-Receptor Interaction and VEGF Signaling Pathways

Lu¹,

Liu²,

Liu³

et al. 2023

IJN

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Introduction Silver nanoparticles (AgNP) are widely used as coating materials. However, the potential risks of AgNP to human health, especially for neural and vascular systems, are still poorly understood. Methods The vascular and neurotoxicity of various concentrations of AgNP in zebrafish were examined using fluorescence microscopy. In addition, Illumina high-throughput global transcriptome analysis was performed to explore the transcriptome profiles of zebrafish embryos after exposure to AgNP. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to elucidate the top 3000 differentially expressed genes (DEGs) between AgNP-exposed and control groups. Results We systematically investigated the neural and vascular developmental toxicities of AgNP exposure in zebrafish. The results demonstrated that AgNP exposure could cause neurodevelopmental anomalies, including a small-eye phenotype, neuronal morphology defects, and inhibition of athletic abilities. In addition, we found that AgNP exposure induces angiogenesis malformation in zebrafish embryos. Further RNA-seq revealed that DEGs were mainly enriched in the neuroactive ligand-receptor interaction and vascular endothelial growth factor (Vegf) signaling pathways in AgNP-treated zebrafish embryos. Specifically, the mRNA levels of the neuroactive ligand-receptor interaction pathway and Vegf signaling pathway-related genes, including si:ch73-55i23.1, nfatc2a, prkcg, si:ch211-132p1.2, lepa, mchr1b, pla2g4aa, rac1b, p2ry6, adrb2, chrnb1 , and chrm1b , were significantly regulated in AgNP-treated zebrafish embryos. Conclusion Our findings indicate that AgNP exposure transcriptionally induces developmental toxicity in neural and vascular development by disturbing neuroactive ligand-receptor interactions and the Vegf signaling pathway in zebrafish embryos.

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Toxicity Risks Associated With the Beta‐Blocker Metoprolol in Marine and Freshwater Organisms: A Review

Love,

Slovisky,

Costa

et al. 2024

Enviro Toxic and Chemistry

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The detection of pharmaceuticals in aquatic ecosystems has generated concern for wildlife and human health over the past several decades. β‐adrenergic blocking agents are a class of drugs designed to treat cardiovascular diseases and high blood pressure. Metoprolol is a second‐generation β1‐adrenergic receptor inhibitor detected in effluent derived from sewage treatment plants. Our review presents an updated survey of the current state of knowledge regarding the sources, occurrence, and toxicity of metoprolol in aquatic ecosystems. We further aimed to summarize the current literature on the presence of metoprolol in various classes of aquatic species and to consider the trophic transfer of these contaminants in marine mammals. The biological impacts of metoprolol have been reported in 20 aquatic organisms, with a primary focus on cardiac function and oxidative stress. Our review reveals that concentrations of metoprolol that cause toxicity in aquatic species are above levels that are typical of marine and freshwater environments. Future studies should investigate the effects of metoprolol at lower concentrations in aquatic organisms. Other recommendations include (1) a further focus on noncardiac endpoints, because computational assessments of currently available molecular data identify gonadotropins, vitellogenin, collagen, and cytokines as potential targets of modulation, and (2) development of adverse outcome pathways for cardiac dysfunction in aquatic species to improve our understanding of molecular interactions and outcomes following exposure. As the next generation of β‐blockers is developed, continued diligence is needed for assessing environmental impacts in aquatic ecosystems to determine their potential accumulation and long‐term effects on wildlife and humans. Environ Toxicol Chem 2024;00:1–14. © 2024 SETAC

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Transcriptional Responses in Adult Zebrafish (Danio rerio) Exposed to Propranolol and Metoprolol

Cited by 16 publications

References 36 publications

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Silver Nanoparticles Cause Neural and Vascular Disruption by Affecting Key Neuroactive Ligand-Receptor Interaction and VEGF Signaling Pathways

Toxicity Risks Associated With the Beta‐Blocker Metoprolol in Marine and Freshwater Organisms: A Review

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