Transcriptional responses of earthworm (Eisenia fetida) exposed to naphthenic acids in soil

Wang, Jie; Cao, Xiaofeng; Sun, Jinhua; Chai, Liwei; Huang, Yi; Tang, Xiaoyan

doi:10.1016/j.envpol.2015.05.006

Cited by 41 publications

(8 citation statements)

References 68 publications

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“…TCTP is related to cell growth and division, which play an important role in organism growth . ANN is related to the reproduction of earthworms, which could induce egg-laying behavior in earthworms. , In the present work, the relative expression quantities of the TCTP gene and ANN gene following Rac -dinotefuran, R -dinotefuran, and S -dinotefuran exposure showed no significant changes on days 2 and 7 compared with those in the control group (Figure C,D). Significant upregulation in TCTP gene and significant downregulation in ANN gene were first observed on days 14, 28, and 14 after exposure to Rac -dinotefuran, R -dinotefuran, and S -dinotefuran compared with those in the control group.…”

Section: Resultsmentioning

confidence: 40%

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Enantioselective Bioaccumulation and Toxicity of the Neonicotinoid Insecticide Dinotefuran in Earthworms (Eisenia fetida)

Liu

Chen

et al. 2018

J. Agric. Food Chem.

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The enantioselective bioaccumulation and toxicity of dinotefuran in earthworms were studied in this study. The results showed that S-dinotefuran accumulated faster than Rac-dinotefuran and R-dinotefuran in earthworms. The acute toxicity of S-dinotefuran was 1.49 and 2.67 times that of the Rac-dinotefuran and R-dinotefuran in artificial soil during 14 days of exposure. At 1.0 mg/kg, the three tested chemicals inhibited the growth and reproduction as well as induced oxidative stress effects in earthworms; however, the toxic effects induced by S-dinotefuran were the most serious. The transcriptome sequencing results showed that S-dinotefuran had stronger interactions to biomacromolecules and influences on the endoplasmic reticulum (ER) than R-dinotefuran, which may be the main reason for enantioselectivities between the two enantiomers. The present results indicated that the risk of S-dinotefuran was higher than that of Rac-dinotefuran and R-dinotefuran in the soil environment to earthworms. Risk assessment of dinotefuran should be evaluated at the enantiomer level.

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Section: Resultsmentioning

confidence: 40%

“…The relative expression level of functional genes was measured using real-time polymerase chain reaction (RT-PCR). The primer sequences of target genes are listed in Table . The total RNA was extracted from one earthworm using the RNApure plant kit (Aidlab, China).…”

Section: Materials and Methodsmentioning

confidence: 99%

Enantioselective Bioaccumulation and Toxicity of the Neonicotinoid Insecticide Dinotefuran in Earthworms (Eisenia fetida)

Liu

Chen

et al. 2018

J. Agric. Food Chem.

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“…coelomic cytolytic factor, i.e., CCF 21 ), antimicrobial proteins (lumbricin I 22 , LBP/BPI 23 , lysozyme 24 ), toll-like receptors functioning in innate immune response against pathogens (mccTLR 25 and sccTLR 26 ), proteins involved in response to oxidative stress (SOD 27 , CAT 27 , and CRT 28 ) or metal stress (PCS 20 ), detoxification proteins (GST 27 and CYP450 29 ), and heat shock protein (HSP70 30 ). However, there was still no holistic overview of earthworm defensive systems.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to the genome, we also performed iTRAQ-based quantitative proteomics analysis to detect differentially expressed proteins of A. corticis and sequenced 16 S rDNA from its intestinal tract after treatment with a pathogenic Escherichia coli stress O157:H7 17,18 to reveal how this earthworm genome functions when facing stresses. Last, we systematically profiled copies of several categories of well determined defensive genes in the genome, including lysins [19][20][21] , antimicrobial proteins [22][23][24] , Toll-like receptors 25,26 , proteins involved in response to oxidative stress 27,28 or metal stress 20 , detoxification proteins 27,29 , and heat shock protein 30 , and found their expansion trend in the genome as well as high diversity in the population of A. corticis.…”

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confidence: 99%

Amynthas corticis genome reveals molecular mechanisms behind global distribution

et al. 2021

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Earthworms (Annelida: Crassiclitellata) are widely distributed around the world due to their ancient origination as well as adaptation and invasion after introduction into new habitats over the past few centuries. Herein, we report a 1.2 Gb complete genome assembly of the earthworm Amynthas corticis based on a strategy combining third-generation long-read sequencing and Hi-C mapping. A total of 29,256 protein-coding genes are annotated in this genome. Analysis of resequencing data indicates that this earthworm is a triploid species. Furthermore, gene family evolution analysis shows that comprehensive expansion of gene families in the Amynthas corticis genome has produced more defensive functions compared with other species in Annelida. Quantitative proteomic iTRAQ analysis shows that expression of 147 proteins changed in the body of Amynthas corticis and 16 S rDNA sequencing shows that abundance of 28 microorganisms changed in the gut of Amynthas corticis when the earthworm was incubated with pathogenic Escherichia coli O157:H7. Our genome assembly provides abundant and valuable resources for the earthworm research community, serving as a first step toward uncovering the mysteries of this species, and may provide molecular level indicators of its powerful defensive functions, adaptation to complex environments and invasion ability.

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“…The formula of this compound is C n H 2n+z O 2 , where n is the number of carbon atoms, and z is zero or a negatively even integer that refers to the loss of a hydrogen [10]. The ecotoxicity of NAs to terrestrial ecosystems, such as rivers and soil, has been confirmed [11,12], and there is evidence that NAs show stronger toxicity in aquatic environments [13]. Compared with environmental media, such as rivers and lakes, the hydrophilic carboxyl group makes NAs more soluble in slightly alkaline seawater, and it has been concluded that NAs will have a faster diffusion rate and wider spread in crude oil-contaminated seawater [14].…”

Section: Introductionmentioning

confidence: 96%

Toxicity of Naphthenic Acids on the Chlorophyll Fluorescence Parameters and Antioxidant Enzyme Activity of Heterosigma akashiwo

et al. 2021

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Petroleum hydrocarbons can serve as a carbon source for marine phytoplankton; so, marine high-acid crude oil pollution events are likely to result in algal outbreaks or harmful algal blooms (HABs) in surface waters. Naphthenic acids (NAs) are the primary acidic component of crude oil, and red tide is of great concern due to its high diffusivity and strong destructive properties. It is important to study the mechanism of the toxic effect of NAs on the typical red tide algae, Heterosigma akashiwo, for the balance and stability of marine algae. The mechanism of NAs’ damage effect was investigated in terms of the antioxidant enzyme activity, cell number, the chlorophyll positive fluorescence parameters, and the cell morphology of microalgae. Experiments confirmed the hormesis of low-concentration (0.5, 2, and 4 mg/L) NAs on Heterosigma akashiwo, and the indicators of high-concentration (8 and 16 mg/L) NA exposures showed inhibition. In this study, the toxic effect of NAs on the target organism showed a clear concentration–dose relationship. The 16 mg/L NAs stress caused severe damage to the morphology and structure of the target biological cells in a short time (96 h), and the population growth decreased. The target organisms showed a staged oxidative stress response to NAs. The behavior in the low-concentration treatment groups showed toxicant excitatory effects on the photosynthetic efficiency and antioxidant enzyme activity of the target organisms. This study provides theoretical and practical data for the development of an important toxicological model of the toxicant’s excitement effects and antioxidant defense mechanisms. In addition, it provides prospective research data for the prediction and avoidance of ecological risk from NA pollution in marine environments.

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Transcriptional responses of earthworm (Eisenia fetida) exposed to naphthenic acids in soil

Cited by 41 publications

References 68 publications

Enantioselective Bioaccumulation and Toxicity of the Neonicotinoid Insecticide Dinotefuran in Earthworms (Eisenia fetida)

Enantioselective Bioaccumulation and Toxicity of the Neonicotinoid Insecticide Dinotefuran in Earthworms (Eisenia fetida)

Amynthas corticis genome reveals molecular mechanisms behind global distribution

Toxicity of Naphthenic Acids on the Chlorophyll Fluorescence Parameters and Antioxidant Enzyme Activity of Heterosigma akashiwo

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