Transcriptional responses of Haemophilus parasuis to iron-restriction stress in vitro

Xie, Qi; Jin, Hui; Luo, Rui; Wan, Yun; Chu, Jie; Zhou, Hufeng; Bi, Shuping; Chen, Huanchun; Zhou, Rui

doi:10.1007/s10534-009-9243-2

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…These findings indicate that “nutritional virulence”, previously reported for other bacterial pathogens [ 41 ], could be an important survival strategy for H. parasuis during lung colonization. Moreover, some of these genes were involved in iron, nitrate and sulfonate acquisition, which are vital for energy generation and have been previously reported to be up-regulated in H. parasuis grown under iron-restriction in vitro [ 42 ]. Furthermore, up-regulation of cytochrome biogenesis, heme transport and some components of the electron transport chain add additional value to the hypothesis that maintenance of anabolic/catabolic balance could be important for survival in the host.…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection

Bello-Ortí

Howell

Tucker

et al. 2015

Vet Res

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Haemophilus parasuis is a common inhabitant of the upper respiratory tract of pigs, and the causative agent of Glässer’s disease. This disease is characterized by polyserositis and arthritis, produced by the severe inflammation caused by the systemic spread of the bacterium. After an initial colonization of the upper respiratory tract, H. parasuis enters the lung during the early stages of pig infection. In order to study gene expression at this location, we sequenced the ex vivo and in vivo H. parasuis Nagasaki transcriptome in the lung using a metatranscriptomic approach. Comparison of gene expression under these conditions with that found in conventional plate culture showed generally reduced expression of genes associated with anabolic and catabolic pathways, coupled with up-regulation of membrane-related genes involved in carbon acquisition, iron binding and pathogenesis. Some of the up-regulated membrane genes, including ABC transporters, virulence-associated autotransporters (vtaAs) and several hypothetical proteins, were only present in virulent H. parasuis strains, highlighting their significance as markers of disease potential. Finally, the analysis also revealed the presence of numerous antisense transcripts with possible roles in gene regulation. In summary, this data sheds some light on the scarcely studied in vivo transcriptome of H. parasuis, revealing nutritional virulence as an adaptive strategy for host survival, besides induction of classical virulence factors.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-015-0225-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection

Bello-Ortí

Howell

Tucker

et al. 2015

Vet Res

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“…DPD (2,2′-dipyridyl) has been widely used to reduce the iron concentration in the medium [16], [30]-[32]. DPD has a six-membered cyclic structure that can decrease the free iron ions by forming stable chelates with iron in the culture medium.…”

Section: Discussionmentioning

confidence: 99%

“…The bacteria from exponential growth phase (OD 630nm ∼0.5) were split into two culture tubes containing 5 mL TSB medium each. To remove iron from the medium, a final concentration 200 µM of 2, 2′-dipyridyl (Sigma) was added into tube A [16], while tube B was kept 2, 2′-dipyridyl free for the normal control. The bacteria were harvested by centrifugation at 8000 rpm for 10 min after the incubation at 37°C, 220 rpm, for 16 hrs.…”

Section: Methodsmentioning

confidence: 99%

Protein Expressions and Their Immunogenicity from Riemerella anatipestifer Cultured in Iron Restriction Medium

Yang

Liu³

et al. 2013

PLoS ONE

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Riemerella anatipestifer was cultured in both iron restriction media and normal media. Two-dimensional gel electrophoresis identified 23 proteins that significantly increased in the iron restriction media. Of them 12 proteins were analyzed with mass spectrography. Nine of 12 proteins belong to 6 different protein families: fibronectin type iii domain protein, secreted subtilase family protein, phosphoglycerate kinase, translation elongation factor, leucine-rich repeat-containing protein, and Galactose-binding domain-like protein. Other 3 proteins were novel with unknown function. Two novel proteins (Riean_1750 and Riean_1752) were expressed in prokaryotic expression systems. The specificities of these 2 novel proteins to R. anatipestifer were confirmed by western-blotting analysis. The ducks immunized with either protein had low mortality challenged by R. anatipestifer, 33.3% and 16.7%, respectively. The ducks developed 100% immunity when immunized with combined Riean_1750 and Riean_1752 proteins. The data suggested 2 novel proteins play important roles in the bacterial survival in the iron restricted environment. They could be used as subunit vaccines of R. anatipestifer.

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“…Another study (48) examined differential expression of H. parasuis genes in response to iron restriction and porcine cerebral spinal fluid using differential-display reverse transcriptase PCR. Nine genes were found to be upregulated during iron restriction, none of which matched any of the upregulated genes reported in the SCOTS study (95). Melnikow et al (47) also included iron limitation as one of the conditions in their microarray experiment.…”

Section: Objectivesmentioning

confidence: 80%

“…As iron acquisition is essential to bacteria attempting to establish and maintain an infection (92), iron restriction is commonly employed to examine gene expression. Thirtysix genes were identified using SCOTS upregulated in a highly virulent strain of H. parasuis under limited iron conditions designed to mimic the in vivo environment of the host (95).…”

Section: Objectivesmentioning

confidence: 99%

Investigations into Haemophilus parasuis virulence: pre-, post-, and pan-genomic perspectives

Mullins

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Transcriptional responses of Haemophilus parasuis to iron-restriction stress in vitro

Cited by 8 publications

References 32 publications

Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection

Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection

Protein Expressions and Their Immunogenicity from Riemerella anatipestifer Cultured in Iron Restriction Medium

Investigations into Haemophilus parasuis virulence: pre-, post-, and pan-genomic perspectives

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