Transcriptional targeting in ovarian cancer cells using the human epididymis protein 4 promoter

Berry, Nicholas B; Cho, Yong Mee; Harrington, Maureen A.; Williams, Stephen D.; Foley, John; Nephew, Kenneth P.

doi:10.1016/j.ygyno.2003.12.024

Cited by 23 publications

(16 citation statements)

References 33 publications

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“…The mechanism of HE4 overexpression in ovarian cancer is not clear. However, it has been demonstrated that the chromosomal region where HE4 is located is frequently amplified in ovarian cancer and breast cancer (Berry et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Tissue CA125 and HE4 Gene Expression Levels Offer Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses

Fawzy

Mohamed²,

Ali³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 99%

Tissue CA125 and HE4 Gene Expression Levels Offer Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses

Fawzy

Mohamed²,

Ali³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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“…33 Similar studies have evaluated the use of the promoter for the WFDC family member SLPI for gene therapy in ovarian and cervical carcinomas. [34][35][36] In summary, our results characterize the distribution of HE4 in normal adult tissues and various malignancies.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of HE4 expression in normal and malignant human tissues

2006

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The HE4 (WFDC2) gene encodes a WAP-type four disulphide core domain-containing protein with a presumptive role in natural immunity. Multiple studies have consistently identified upregulation of HE4 gene expression in carcinomas of the ovary; however, the expression in normal and malignant adult tissues has not been examined in detail. Here, we examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituitary gland, thyroid, and kidney. In a series of 175 human adult tumors, gene expression was highest in ovarian serous carcinomas. However, adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas had moderate or high levels of HE4 expression. Using tissue microarrays and full tissue sections of normal and 448 neoplastic tissues, HE4 immunoreactivity was found in normal glandular epithelium of the female genital tract and breast, the epididymis and vas deferens, respiratory epithelium, distal renal tubules, colonic mucosa, and salivary glands, consistent with HE4 gene expression. In addition to consistent positivity in ovarian carcinoma, some pulmonary, endometrial, and breast adenocarcinomas, mesotheliomas, and less often, gastrointestinal, renal and transitional cell carcinomas were also positive. Knowledge of the expression patterns of HE4 in our survey is useful for application in histopathologic diagnosis, and should be taken into consideration in future studies that examine the role of HE4 as a serological tumor biomarker or as a target for gene-based therapy.

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“…We also found that HE4 translocated to the nuclei or nucleoli of SKOV3 and OVCAR8 wild type cells following stimulation with 17b-estradiol or sub-toxic concentrations of fulvestrant or tamoxifen. This suggested that HE4 signaling is responsive to estrogens and antiestrogens; indeed, genomic analysis of the HE4 promoter region identified several hormonal responsive elements including estrogen receptor (ER) and RAR-related orphan receptor A (RORA), which may play a role in HE4 upregulation in ovarian cancer 21 . Confocal fluorescence for ER-a also showed that ER-a internalized to the nucleus upon stimulation with estrogens and antiestrogens.…”

Section: Discussionmentioning

confidence: 99%

HE4 expression is associated with hormonal elements and mediated by importin-dependent nuclear translocation

Lokich

Han

Romano

et al. 2015

Gynecologic Oncology

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Transcriptional targeting in ovarian cancer cells using the human epididymis protein 4 promoter

Cited by 23 publications

References 33 publications

Tissue CA125 and HE4 Gene Expression Levels Offer Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses

Tissue CA125 and HE4 Gene Expression Levels Offer Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses

Comprehensive analysis of HE4 expression in normal and malignant human tissues

HE4 expression is associated with hormonal elements and mediated by importin-dependent nuclear translocation

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