Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation

Abstract

Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony. In this study, on the basis of transcriptome sequencing, differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation. A total of 31.63 Gb raw data were generated and 120,188 unigenes (mean length of 911.98 bp) were annotated according to six databases (NR, NT, GO, KEGG, COG, and Swiss-Prot). Analyses of the ungerminated root primordium period, induced root primordium period, and root formation period detected 8,232, 6,907, and 10,687 differentially expressed genes related to 133, 132, and 133 metabolic pathways, respectively. Two significantly differentially expressed genes (Unigene13430_All and CL10096.Contig1_All) were associated with the auxin pathway. The full-length Unigene13430_All coding sequence (843 bp) encoded 280 amino acids, whereas the full-length CL10096.Contig1_All coding sequence (1,470 bp) encoded 489 amino acids. Unigene13430_All and CL10096.Con-tig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19. The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings, cuttings and grafted seedlings were significant different. PsARF19 promoted root development, it might be a regulatory gene related to the formation of tree peony roots, while PsIAA27 inhibited lateral root development, and it might be involved in controlling auxin sensitivity during root formation. The results of this study may form the basis of future investigations on the mechanism mediating peony root formation. The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes.

Functional Analysis of PsARRO−1 in Root Development of Paeonia suffruticosa

“…In a preliminary experiment, we used the root systems of live seedlings of Paeonia suffruticosa variety 'Fengdanbai' at the R1 (root primordium ungerminated period), R2 (root primordium induction period), and R3 (lateral root formation period) as materials to establish the transcriptome database of root development of Paeonia suffruticosa under sandy loam cultivation [42]. In this database, we identified a PsARRO−1 gene (GenBank accession number KJ620008), which was differentially expressed at both the R1/R2 and R2/R3 stages, compared to the expression of root−associated genes such as ARFs, IAAs, WRKYs, WUS, and WOXs in this database, as shown in Figure 1.…”

RNA Sequencing Analysis and Verification of Paeonia ostii ‘Fengdan’ CuZn Superoxide Dismutase (PoSOD) Genes in Root Development

“…This highlights the significance of antioxidant enzyme-related genes in the development of adventitious roots [21]. In tree peonies, certain genes related to plant hormones were identified through transcriptome analysis, and these genes were found to be key in promoting the formation of root primordia [22,23]. In short, the research has mainly focused on the molecular regulation mechanism of hormones on rooting, but there are few studies on the effects of antioxidant enzyme-related genes on rooting.…”

Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential

“…In the past, various plant parts of peonies (seeds, flowers, leaves and roots, as well as whole plants) have been widely used to treat various diseases, pain (head, stomach, and eyes) [4], problem regarding female genitals (dysmenorrhea and amenorrhea) [5], aches, neurological diseases (spasm and epilepsy) [6], infectious diseases (carbuncles) [4], urinary system diseases, and inflammation (otitis media, appendicitis, and gastritis) [6], as well as trauma. The petals of herbaceous peonies have been a subject of very few studies, and it has been shown that they possess skin-beneficial biological activities [3,7].…”