Transcriptome analysis confers a complex disease resistance network in wild rice Oryza meyeriana against Xanthomonas oryzae pv. oryzae

Cheng, Xiaoyu; He, Bin; Chen, Lin; Xiao, Shuqi; Fu, Jian; Chen, Yue; Yu, Tong; Cheng, Zhihui; Hong, Feng

doi:10.1038/srep38215

Cited by 27 publications

(13 citation statements)

References 54 publications

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“…9), suggesting their role in conferring DM resistance. The similar results have been reported in many plant-pathogen interactions [40,83]. The actual role of these genes in conferring the defense mechanism against DM of Isabgol remains to be explored.…”

Section: Realtime Gene Expression Analysissupporting

confidence: 84%

“…However, till date, no reports are on R-genes specific to DM resistance in Isabgol. We found, CDSs were reported in plant-pathogen interactions [83]. Identification and molecular charectization of Rgenes of plants will help developing disease resistant crops.…”

Section: Realtime Gene Expression Analysismentioning

confidence: 62%

“…The phenylpropanoid pathway serves as a rich source of metabolites in plants, being required for the biosynthesis of lignin, and serving as a starting point for the production of many other important compounds, such as the flavonoids and coumarins which plays important role in plant defense [82][83]. There were 14 genes involved in phenylpropanoid biosynthetic pathway in plants [84].…”

Section: Realtime Gene Expression Analysismentioning

confidence: 99%

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Comparative Transcriptome Analysis Uncovers Genes and Pathways Relating to Downy Mildew Resistance in Isabgol (Plantago ovata Forsk.)

Manivel¹,

Patel²,

Mathew³

et al. 2020

Preprint

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BackgroundIsabgol (Plantago ovata Forsk.) downy mildew (DM), caused by obligate oomycete pathogen Peronospora plantaginis Underwood., is the single most damaging disease of Isabgol. However, reports on the genes and pathways involved in mediating resistance are still unknown.ResultsIn the present study, transcriptomic analysis was carried out by next generation sequencing of DM infected and uninfected leaves of resistant and susceptible genotypes to understand the genetic mechanisms underlie the host-plant resistance. A total of 33.47 million reads were generated and de novo assembled and annotated. The assembly using Trinity yielded 38803, 40175, 45451 and 39533 non-redundant transcript contigs, respectively in susceptible infected, Susceptible uninfected, Resistant infected and resistant uninfected samples. More than 90% of coding DNA sequence (CDS) predicted were annotated using BLAST search. Isabgol transcriptome showed the highest similarity to Sesamum indicum (41-59%) followed by Erythranthe guttata (10-13%). Putative CDS encoding Pathogen associated Molecular Pattern (PAMP)-triggered immunity (PTI), Effector-Triggered Immunity (ETI), Cell wall degrading enzymes, Phytohormone signalling and Phenylpropanoid biosynthesis pathways involved in host-pathogen interaction were identified in addition to the identification of several candidate resistance (R) genes enriched in response to DM infection. We identified significantly differentially expressed genes (DEGs) genes; 6928 DEGs in resistant uninfected (RU) vs resistant infected (RI) of DPO-185 (resistant) genotype and 8779 susceptible uninfected (SU) vs susceptible infected (SI) of DPO-14 (susceptible) genotype. Expression of 11 genes involved in plant defense quantified accordingly using RT-qPCR.ConclusionsThis study for the first time provides the glimpse of transcriptional responses to the DM resistance in Isabgol. The genes and pathways will be helpful in further investigating the molecular mechanisms associated with DM disease resistance in Isabgol and to develop control mechanisms accordingly.

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Section: Realtime Gene Expression Analysissupporting

confidence: 84%

Section: Realtime Gene Expression Analysismentioning

confidence: 62%

Section: Realtime Gene Expression Analysismentioning

confidence: 99%

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Comparative Transcriptome Analysis Uncovers Genes and Pathways Relating to Downy Mildew Resistance in Isabgol (Plantago ovata Forsk.)

Manivel¹,

Patel²,

Mathew³

et al. 2020

Preprint

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“…Nucleotide sequence consistency of them was 90.61% and 78.98%, respectively. Plants evolved a complex innate immune system that recognized speci c pathogens via a speci c group of gene products called resistance (R) proteins [50]. RGAs were involved in plant disease resistance [51,52].…”

Section: Discussionmentioning

confidence: 99%

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

Yao¹,

Wang²,

Yao³

et al. 2020

Preprint

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Background Tibetan hulless barley is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Results Here, we compared the miRNA profiles before and after BLS in two Tibetan hulless barley genotypes: Z1141, a BLS-sensitive wild variety, and Kunlun14, a BLS-tolerant hybrid variety. A total of 36 conserved and 56 novel miRNAs were identified. Of these, 10 conserved and 10 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Kunlun14, respectively, while 3 conserved and 5 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Z1141, respectively. A total of 24 miRNAs were found in Z1141 and Kunlun14, and a further 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among the 546 candidate genes, 131 had significant differences in expression between the normal and infected leaves of Kunlun14 and Z1141. Gene ontology, pathway, and Blast analyses indicated 10 candidate target genes that were involved in the barley disease resistance. These 10 candidate target genes may be regulated by 7 miRNAs. According to quantitative real-time PCR results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Conclusions The miRNAs and their target genes expressed in Tibetan hulless barley were identified and found to be associated with BLS resistance. Thus, these miRNAs and their target genes may be exploited via breeding programs or genetic engineering to improve BLS resistance in Tibetan hulless barley.

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“…Transcriptomics has been used successfully in the management of Xanthomonas oryzae on rice (Cheng et al, 2016). Genome editing technologies have been used successfully in enhancing plant resistance to phytopathogens (Andolfo et al, 2016).…”

Section: Transcriptomics As An Approach Of Managing Plant Diseasesmentioning

confidence: 99%

Application of molecular and biotechnological techniques in plant disease management: A review

Dayou¹,

Mwangi²,

Egesa³

et al. 2018

Afr. J. Biotechnol.

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Plant diseases are a major challenge in crop production. They are caused by nematodes, bacteria, fungi, viruses as well as plant nutritional factors. Diseases interfere with the normal physiological and metabolic processes of plants. This results in various effects including wilting, stunting, yellowing and death of plant tissues and organs. Crop losses due to diseases manifest in form of reduced yield, poor quality produce, and reduced post-harvest storage. Past research has brought to the limelight the continuous capacity of pathogens to revert to new pathotypes and strains, some that break resistant varieties or are less sensitive to chemical control products. Currently, farmers are advised to combine several plant disease management practices, a strategy known as integrated plant disease management. Such strategies include crop rotation, use of disease free planting materials, field sanitation, and chemical methods as well as use of resistant varieties. However, some of these methods are expensive and substantially increase the cost of production. Development in molecular biology and biotechnology found application in plant disease management. This ranges from identification, diagnosis to control through gene transfer, mutation breeding and RNA interference, among others. In this paper, the current developments in the application of molecular techniques and biotechnology to manage plant diseases, outlining their possible future application and potential for enhanced plant disease management.

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Transcriptome analysis confers a complex disease resistance network in wild rice Oryza meyeriana against Xanthomonas oryzae pv. oryzae

Cited by 27 publications

References 54 publications

Comparative Transcriptome Analysis Uncovers Genes and Pathways Relating to Downy Mildew Resistance in Isabgol (Plantago ovata Forsk.)

Comparative Transcriptome Analysis Uncovers Genes and Pathways Relating to Downy Mildew Resistance in Isabgol (Plantago ovata Forsk.)

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

Application of molecular and biotechnological techniques in plant disease management: A review

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