Transcriptome analysis of airborne PM 2.5 -induced detrimental effects on human keratinocytes

Kim, Hyoung-June; Bae, Il-Hong; Son, Eui Dong; Park, Juyearl; Cha, Nari; Na, Hye-Won; Jung, Changjo; Go, You-Seak; Kim, Dae-Yong; Lee, Tae Ryong; Shin, Dong Wook

doi:10.1016/j.toxlet.2017.03.010

Cited by 47 publications

(58 citation statements)

References 53 publications

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“…The present results showed that PM2.5 exposure inhibited cell migration, and attenuated the melanization process in both PIG1 and PIG3V cells. Melanocyte migration and melanogenesis are controlled by cytokines (such as SCF and bFGF) secreted by keratinocytes (39,40). The exosomes secreted by keratinocytes could be involved in melanin synthesis in melanocytes (31,41).…”

Section: Discussionmentioning

confidence: 99%

PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes

et al. 2020

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Recent growing evidence suggested that particulate matter 2.5 (PM2.5) has strong toxic effects on skin systems. However, the possible effects and the mechanisms of PM2.5 on vitiligo remain poorly understood. Therefore, the present study aimed to further investigate the effects and possible mechanisms of PM2.5 on vitiligo. Human keratinocytes (HaCaT cells) and human melanocytes (PIG1 cells and PIG3V cells) were exposed to PM2.5 (0-200 µg/ml) for 24 h. The cell viability of the three cell lines was measured by a Cell Counting Kit-8 assay. The secretions of stem cell factor (SCF) and basic fibroblast growth factor (bFGF) in HaCaT cells were evaluated by ELISA. The melanin contents, cellular tyrosinase activity, apoptosis, cell migration, malondialdehyde (MDA) contents, superoxide dismutase (SOD) levels, glutathione peroxidase (GSH-Px) levels and related protein expressions in PIG1 cells and PIG3V cells were evaluated by a NaOH assay, DOPA assay, Annexin V-FITC/Propidium Iodide staining, MDA assay, SOD assay, GSH-Px assay and western blotting, respectively. It was demonstrated that PM2.5 exposure inhibited cell viability of all three cell lines (HaCaT, PIG1 and PIG3V cells). PM2.5 exposure attenuated the secretions of SCF and bFGF in HaCaT cells. Moreover, PM2.5 exposure attenuated the activation of tyrosinase and melanogenesis, inhibited cell migration, and induced apoptosis and oxidative stress injury in PIG1 cells and PIG3V cells. In addition, PM2.5 exposure caused upregulated cytosolic cytochrome C and activated caspase-3 in PIG1 cells and PIG3V cells. Furthermore, PM2.5 exposure activated the nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 signaling pathway. The present results suggested that PM2.5 exposure could inhibit the secretions of SCF and bFGF in keratinocytes, and cause oxidative stress injury and melanin metabolic disorder in melanocytes. Therefore, PM2.5 could be a new risk factor for vitiligo. PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes

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Section: Discussionmentioning

confidence: 99%

PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes

et al. 2020

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“…Of these, PM has recently come to be regarded as a main culprit that negatively affects human health. PM 10 and PM 2.5 are defined as the fractions of particles with aerodynamic diameters smaller than 10 and 2.5 μm, respectively . These sizes of PM are small enough to reach alveoli through the nose and respiratory system and can be absorbed in the blood .…”

Section: Introductionmentioning

confidence: 99%

Effects of particulate matter on healthy human skin: a panel study using a smartphone application measuring daily skin condition

Park

Lee

2019

Acad Dermatol Venereol

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Background The influence of particulate matter (PM) on human health has recently attracted attention. However, most previous studies have been limited to diseased skin. Objectives To evaluate the effects of PM on healthy skin. Methods The study enrolled 188 healthy volunteers without any skin disease during winter and early spring. Every day for 14 days, they recorded their skin states using a smartphone application, which automatically scored facial skin condition in terms of wrinkle, brown‐spot and trouble indexes based on a selfie. Subjects recorded information about their personal lifestyles such as sleeping time and outdoor activity. Air pollution and meteorological information were collected during the same period as well. We analysed the correlation between daily skin condition and environmental factors. Results Overall, 2625 person‐days were obtained for 3 months from February to April. Among the various factors analysed in this study, temperature and PM showed statistically significant relationships with the daily condition. Multivariable analysis showed a cumulative effect, as PM2.5 from 8 to 3 days prior to when daily skin condition was checked had a negative correlation with wrinkle index (P < 0.05). Conclusions Inadequate outdoor temperature can immediately impinge on the skin state of even healthy people. PM2.5 may contribute to skin ageing gradually rather than immediately.

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“…From 2015 to the present (2017) the number of published reports on air pollution and skin increased substantially and covered topics such as: (a) indoor air pollution from cooking accelerated skin aging in Chinese women, and atopic dermatitis increased from other indoor pollutants, such as polyvinyl chloride (PVCs) from wallpaper [51,52]; (b) decreased skin barrier function and increased facial hyperpigmentation (lentigines) from ambient PMs that are known to increase inflammatory skin diseases, ROS and matrix metalloproteinases (MMPs) that degrade collagen [53][54][55][56][57]; and (c) fine airborne PM activated the aryl hydrocarbon receptor (AHR) that resulted in detrimental effects on human keratinocytes [58,59]. Finally, in brief, two recent reviews have summarized the recognized negative impact of ambient air pollution on skin health, which suggested four general mechanisms by which this occurs: (1) generation of ROS; (2) induction of the inflammatory cascade and subsequent impairment of the skin barrier; (3) activation of the AHR which stimulates the expression skin aging genes such as MMPs that degrade collagen; and (4) alterations to the skin microflora [60,61].…”

Section: Air and Water Pollution And The Generation Of Rosmentioning

confidence: 99%

Equol’s Anti-Aging Effects Protect against Environmental Assaults by Increasing Skin Antioxidant Defense and ECM Proteins While Decreasing Oxidative Stress and Inflammation

Lephart

2018

Cosmetics

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Environmental pollutants represent a major problem worldwide that cannot be passively avoided. It is known that skin sensitivities can result from environmental assaults, such as toxins and pollutants in air and water. Additionally, dermal assaults from wind and exposure to seasonal cold temperatures are known. All of these environmental assaults are associated with oxidative stress and the intracellular generation of reactive oxygen species (ROS), which damage DNA, lipids, proteins and mitochondrial function. Additionally, the influence of diet on dermal health and, especially, antioxidant defense in skin function are well established. In this regard, environmental pollution worldwide has generated a high demand for anti-pollution personal care products to protect the skin against the daily exposure of airborne toxins and various other assaults. Major cosmetic companies have anti-pollution personal care products but, in general, the products are formulated with commonly used active ingredients that have been retooled with market strategies to address current environmental pollution treatments. Equol is a new botanical active ingredient compound for skin applications. It has a polyphenolic chemical structure found in plant and food products, and is also classified as an isoflavonoid. Moreover, equol appears to address the need for an active ingredient in personal care products to protect against pollution assaults by increasing antioxidant defense, while inhibiting oxidative stress and inflammation. Separate sections covering equol's enhanced (a) delivery mechanism into human skin; (b) antioxidant effects via Nrf2 activation; (c) effects on extracellular matrix proteins like collagen and elastin and; (d) protection against oxidative stress and inflammation are presented.

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Transcriptome analysis of airborne PM 2.5 -induced detrimental effects on human keratinocytes

Cited by 47 publications

References 53 publications

PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes

PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes

Effects of particulate matter on healthy human skin: a panel study using a smartphone application measuring daily skin condition

Equol’s Anti-Aging Effects Protect against Environmental Assaults by Increasing Skin Antioxidant Defense and ECM Proteins While Decreasing Oxidative Stress and Inflammation

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