Transcriptome analysis of Arabidopsis mutants suggests a crosstalk between ABA, ethylene and GSH against combined cold and osmotic stress

Kumar, Deepak; Hazra, Saptarshi; Datta, R. K.; Chattopadhyay, Sharmila

doi:10.1038/srep36867

Cited by 42 publications

(23 citation statements)

References 57 publications

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“…The subtle effects of the fip1‐2 mutation on poly(A) site choice belie the more dramatic phenotypes seen in the mutant, such as the diminished capacity to develop LRs, leaf growth, the tolerance to salt, and the increased sensitivity to cadmium and ABA. Transcriptomic and proteomic analyses have identified a large number of genes/proteins involved in root development and the adaptation of plants to salt stress or ABA responses (Brady et al ., , ; Dinneny et al ., ; Li et al ., ; Guo et al ., ; Ding et al ., ; Kumar et al ., ). It is possible that among the numerous changes in poly(A) site choice that are seen in the fip1‐2 mutant, some of them affect important regulatory genes, which might have a large impact on plant development and responses; however, our results (Figure ) suggest an interesting alternative.…”

Section: Discussionmentioning

confidence: 97%

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

et al. 2019

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Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5 0 untranslated regions (5 0 -UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

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Section: Discussionmentioning

confidence: 97%

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

et al. 2019

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“…Here, LOX2 expression was reduced in the OsTCP19 overexpressors and ABA signaling genes were upregulated (Mukhopadhyay et al, 2015). Recent experiments revealed up- and down-regulation of several TCPs in Arabidopsis under osmotic stress, although a functional analysis of their role in response to osmotic stress has not been done yet (Kumar et al, 2016). In summary, these few results are first indications that TCPs are no mere static regulators of development, but that they do directly translate environmental signals into growth regulation ( Figure 4 ).…”

Section: Mediating Environmental Signals Into Growth Responsesmentioning

confidence: 99%

“… Schematic figure depicting the diversity of environmental signals that affect TCP functions in plants (Mukhtar et al, 2011; Sugio et al, 2011; Balsemão-Pires et al, 2013; González-Grandío et al, 2013; Niwa et al, 2013; Viola et al, 2013, 2016; Guan et al, 2014; Hu et al, 2014; Kim et al, 2014; Mukhopadhyay et al, 2015; Nicolas et al, 2015; Kumar et al, 2016) .…”

Section: Mediating Environmental Signals Into Growth Responsesmentioning

confidence: 99%

TCP Transcription Factors at the Interface between Environmental Challenges and the Plant’s Growth Responses

2016

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Plants are sessile and as such their reactions to environmental challenges differ from those of mobile organisms. Many adaptions involve growth responses and hence, growth regulation is one of the most crucial biological processes for plant survival and fitness. The plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, PCF1 (TCP) transcription factor family is involved in plant development from cradle to grave, i.e., from seed germination throughout vegetative development until the formation of flowers and fruits. TCP transcription factors have an evolutionary conserved role as regulators in a variety of plant species, including orchids, tomatoes, peas, poplar, cotton, rice and the model plant Arabidopsis. Early TCP research focused on the regulatory functions of TCPs in the development of diverse organs via the cell cycle. Later research uncovered that TCP transcription factors are not static developmental regulators but crucial growth regulators that translate diverse endogenous and environmental signals into growth responses best fitted to ensure plant fitness and health. I will recapitulate the research on TCPs in this review focusing on two topics: the discovery of TCPs and the elucidation of their evolutionarily conserved roles across the plant kingdom, and the variety of signals, both endogenous (circadian clock, plant hormones) and environmental (pathogens, light, nutrients), TCPs respond to in the course of their developmental roles.

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“…Subsequent studies showed that glutathione induces the transcription of genes associated with ethylene biosynthesis in a WRKY33-dependent manner ( Datta et al, 2015 ). Comparative transcriptome and proteome analyses by using ethylene-insensitive, abscisic acid (ABA), and glutathione mutants suggested a crosstalk among ethylene, ABA, and glutathione in inducing stress-responsive genes and proteins to mitigate osmotic and cold stress in Arabidopsis ( Kumar et al, 2016 ). However, little is known about the relationship between glutathione and mild abiotic stress, especially nutrient stress.…”

Section: Introductionmentioning

confidence: 99%

Effects of Combined Low Glutathione with Mild Oxidative and Low Phosphorus Stress on the Metabolism of Arabidopsis thaliana

et al. 2017

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Plants possess highly sensitive mechanisms that monitor environmental stress levels for a dose-dependent fine-tuning of their growth and development. Differences in plant responses to severe and mild abiotic stresses have been recognized. Although many studies have revealed that glutathione can contribute to plant tolerance to various environmental stresses, little is known about the relationship between glutathione and mild abiotic stress, especially the effect of stress-induced altered glutathione levels on the metabolism. Here, we applied a systems biology approach to identify key pathways involved in the gene-to-metabolite networks perturbed by low glutathione content under mild abiotic stress in Arabidopsis thaliana. We used glutathione synthesis mutants (cad2-1 and pad2-1) and plants overexpressing the gene encoding γ-glutamylcysteine synthetase, the first enzyme of the glutathione biosynthetic pathway. The plants were exposed to two mild stress conditions—oxidative stress elicited by methyl viologen and stress induced by the limited availability of phosphate. We observed that the mutants and transgenic plants showed similar shoot growth as that of the wild-type plants under mild abiotic stress. We then selected the synthesis mutants and performed multi-platform metabolomics and microarray experiments to evaluate the possible effects on the overall metabolome and the transcriptome. As a common oxidative stress response, several flavonoids that we assessed showed overaccumulation, whereas the mild phosphate stress resulted in increased levels of specific kaempferol- and quercetin-glycosides. Remarkably, in addition to a significant increased level of sugar, osmolytes, and lipids as mild oxidative stress-responsive metabolites, short-chain aliphatic glucosinolates over-accumulated in the mutants, whereas the level of long-chain aliphatic glucosinolates and specific lipids decreased. Coordinated gene expressions related to glucosinolate and flavonoid biosynthesis also supported the metabolite responses in the pad2-1 mutant. Our results suggest that glutathione synthesis mutants accelerate transcriptional regulatory networks to control the biosynthetic pathways involved in glutathione-independent scavenging metabolites, and that they might reconfigure the metabolic networks in primary and secondary metabolism, including lipids, glucosinolates, and flavonoids. This work provides a basis for the elucidation of the molecular mechanisms involved in the metabolic and transcriptional regulatory networks in response to combined low glutathione content with mild oxidative and nutrient stress in A. thaliana.

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Transcriptome analysis of Arabidopsis mutants suggests a crosstalk between ABA, ethylene and GSH against combined cold and osmotic stress

Cited by 42 publications

References 57 publications

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

TCP Transcription Factors at the Interface between Environmental Challenges and the Plant’s Growth Responses

Effects of Combined Low Glutathione with Mild Oxidative and Low Phosphorus Stress on the Metabolism of Arabidopsis thaliana

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