Transcriptome analysis of atemoya pericarp elucidates the role of polysaccharide metabolism in fruit ripening and cracking after harvest

Chen, Jingjing; Duan, Yueqiang; Hu, Yue; Li, Weiming; Sun, Dequan; Hu, Huigang; Xie, Jianhua

doi:10.1186/s12870-019-1756-4

Cited by 53 publications

(45 citation statements)

References 47 publications

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“…In line to this, the high level of galacturonate in cracking-resistant cultivars (Fig. 4c) could also be advocated as a precursor compound for protopectin, which may contribute to early pectin formation [29].…”

Section: Discussionmentioning

confidence: 67%

“…Indeed, overexpression of trehalose biosynthetic genes results in a more sensitive reaction of the stomatal guard cells and closing of the stomata under water stress conditions [28]. Interestingly, an up-regulation of several genes involved in trehalose metabolism has been observed in African Pride atemoya during various cracking stages [29]. Taking into account that the osmotic water uptake through the skin is an important factor for rain-induced sweet cherries cracking [30] along with the excellent capacity of trehalose to protect membranes and proteins from degradation [31], we assume that trehalose could not be only directly involved in cracking but can also regulate this process mediating the crosstalk with osmoprotectant-related compounds as, for example, with sugars [32].…”

Section: Discussionmentioning

confidence: 99%

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Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2020

Preprint

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Background: Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivocracking assay, the 'Christensen method' (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions.Herein, we have designed and evaluated a cracking protocol, named 'Waterfall method', in which fruits are continuously wetted under controlled conditions. Results: The application of this method alone, or in combination with 'Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC-MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index-metabolite correlations were identified. Conclusions:We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.Additional file 1: Supplementary Table S1. Physiological traits of seventeen cherry cultivars at harvest and MANOVA output. Supplementary Table S2. Texture properties, main cracking of cultivars at harvest and MANOVA output. Additional file 2:Additional file 3: Supplementary Table S3. Quantitative results of skin primary metabolite analysis and MANOVA output. Supplementary Table S4. Quantitative results of skin secondary metabolite analysis and MANOVA output. Additional file 4:

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2020

Preprint

View full text Add to dashboard Cite

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Section: Physiological Traits and Skin Metabolites In Relations To Crmentioning

confidence: 67%

“…Indeed, overexpression of trehalose biosynthetic genes results in a more sensitive reaction of the stomatal guard cells and closing of the stomata under water stress conditions [25]. Interestingly, an up-regulation of several genes involved in trehalose metabolism has been observed in African Pride atemoya during various cracking stages [26]. Taking into account that the osmotic water uptake through the skin is an important factor for rain-induced sweet cherries cracking [27] along with the excellent capacity of trehalose to protect membranes and proteins from degradation [28], we assume that trehalose could not be only directly involved in cracking but can also regulate this process mediating the crosstalk with osmoprotectant-related compounds as, for example, with sugars [29].…”

Section: Physiological Traits and Skin Metabolites In Relations To Crmentioning

confidence: 99%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test has been questioned. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results: The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC-MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions: We have established an efficient cracking protocolwhich may facilitate breeding for new sweet cherry cultivars with high resistance tocracking.

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“…Fruit cracking is a complex physiological process that is controlled by numerous genes working together, rather than a single gene directly controlling the process [18,45]. Researchers have found that several cell wall modi cation genes, including β-GAL, β-GLU, PE, and PG are differentially expressed in cracked fruits compared with levels in non-cracking litchi fruits [21].…”

Section: Candidate Degs and Daps May Play Key Roles In Fruit Pericarpmentioning

confidence: 99%

Integrative transcriptome and proteome analyses provide new insights into different stages of Akebia trifoliata fruit cracking during ripening

Niu

Shi

Huang

et al. 2020

Preprint

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Background: Akebia trifoliata (Thunb.) Koid may have applications as a new source of biofuels owing to its high seed count, seed oil content, and in-field yields. However, the pericarp of A. trifoliata cracks longitudinally during fruit ripening, which increases the incidence of pests and diseases and can lead to fruit decay and deterioration, resulting in significant losses in yield. Few studies have evaluated the mechanisms underlying A. trifoliata fruit cracking. Results: In this study, by observing the cell wall structure of the pericarp, we found that the cell wall became thinner and looser and showed substantial breakdown in the pericarp of cracking fruit compared with that in non-cracking fruit. Moreover, integrative analyses of transcriptome and proteome profiles at different stages of fruit ripening demonstrated changes in the expression of various genes and proteins after cracking. Furthermore, the mRNA levels of 20 differentially expressed genes were analyzed, and parallel reaction monitoring analysis of 20 differentially expressed proteins involved in cell wall metabolism was conducted. Among the molecular targets, pectate lyases and pectinesterase, which are involved in pentose and glucuronate interconversion, and β-galactosidase 2, which is involved in galactose metabolism, were significantly upregulated in cracking fruits than in non-cracking fruits. This suggested that they might play crucial roles in A. trifoliata fruit cracking. Conclusions: Our findings provided new insights into potential genes influencing the fruit cracking trait in A. trifoliata and established a basis for further research on the breeding of cracking-resistant varieties to increase seed yields for biorefineries.

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Transcriptome analysis of atemoya pericarp elucidates the role of polysaccharide metabolism in fruit ripening and cracking after harvest

Cited by 53 publications

References 47 publications

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Integrative transcriptome and proteome analyses provide new insights into different stages of Akebia trifoliata fruit cracking during ripening

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