Transcriptome analysis of Chironomus sulfurosus larvae living in acidic environments: Insights into molecular mechanisms for acid tolerance

Fujii, Sotaro; Kobayashi, Satoru; Tettey, Pamela Afi; Lisdiana, Lisa; Saito, Hidetoshi; Kawai, Kazuhiro; Sambongi, Yoshihiro

doi:10.1016/j.jinsphys.2021.104288

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…Even in acid-tolerant larval microbiomes, acid-tolerant or acidophilic microorganisms were not found. We previously demonstrated that the body fluid pH of acid-tolerant C. sulfurosus larvae acclimated at pH 7 and pH 2 was nearly neutral ( Fujii et al , 2021 ). Therefore, acid-tolerant or acidophilic microorganisms may not necessarily exist in acid-tolerant Chironomus larvae, and the specific microbiome composition observed in the present study appears to have been affected by factors other than body fluid pH.…”

Section: Resultsmentioning

confidence: 99%

“…riparius larvae may be due to a buffering capacity facilitated by high levels of hemoglobin and elevated ATPase activity ( Kawai et al , 2019 ). The expression of cuticle protein genes was recently shown to be up-regulated in C. sulfurosus larvae acclimated to acidic conditions, which conferred a physical barrier against an external acidic environment ( Fujii et al , 2021 ). Additionally, C. sulfurosus larvae display chemotaxis towards acidic areas, indicating a response to protons ( Fujii et al , 2021 ).…”

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confidence: 99%

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Species-specific Microorganisms in Acid-tolerant <i>Chironomus</i> Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis

Fujii,

Kawai,

Sambongi

et al. 2023

Microb. Environ.

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To obtain a more detailed understanding of organismal acid tolerance, the larval microbiomes of 11 Chironomus species collected from acidic or neutral pH areas in Japan and reared at pH 7–8 under laboratory conditions were systematically compared using an amplicon sequencing analysis. Evenness values were lower for the larval microbiomes of acid-tolerant Chironomus cf. riparius , Chironomus fusciceps , and Chironomus sulfurosus than for eight acid-sensitive species based on an alpha diversity analysis. The lower evenness observed suggested a biased abundance of microorganisms, which was consistent with the identification of Chironomus species-specific microorganisms (such as Agromyces mediolanus and Comamonas odontotermitis related bacteria) with high abundance in acid-tolerant larvae. The abundance of specific microorganisms was also high in the microbiome of acid-tolerant larvae of Chironomus acerbiphilus reared at pH 4, but not in that of acid-sensitive larvae. Based on a PICRUSt2 analysis, genes involved in saccharide transport were less abundant in the microbiome of acid-tolerant larvae than in that of acid-sensitive larvae, indicating nutrient-poor acidic environments. Although these results were obtained from single datasets, acid-tolerant larvae appeared to establish Chironomus species-specific interactions with microorganisms independent of saccharides, in contrast to acid-sensitive larvae. The present study is the first step towards understanding organismal acid tolerance.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Species-specific Microorganisms in Acid-tolerant <i>Chironomus</i> Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis

Fujii,

Kawai,

Sambongi

et al. 2023

Microb. Environ.

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“…Knockdown of CHS1a expression using RNA interference (RNAi) results in significant phenotypic defects and mortality in treated larvae [78]. Furthermore, the CHS2 transcript is predominantly found in the larval midgut [79], and its expression is positively correlated with the total chitin content during the insect's development. Other crucial enzymes involved in the chitin biosynthesis pathway, such as glucose-6-phosphate isomerase and UDP-N-acetylglucosamine pyrophosphorylase, have also been cloned and characterized in drosophila [80].…”

Section: Exploration Of Molecular Mechanismsmentioning

confidence: 99%

Understanding the Invasion, Ecological Adaptations, and Management Strategies of Bactrocera dorsalis in China: A Review

Jaffar,

Rizvi,

2023

Horticulturae

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Bactrocera dorsalis (Hendel, 1912) (Diptera: Tephritidae), commonly known as the oriental fruit fly, is a highly destructive pest that globally infests fruits and vegetables, resulting in significant annual economic losses. Initially detected in Taiwan Island, it has rapidly expanded its distribution range to various regions in mainland China since the 1980s, with a continuous northward spread. To mitigate the damage caused by this pest, extensive efforts have been undertaken to comprehend its ecological and physiological adaptations and develop management strategies. This review article provides an overview of the invasion history of B. dorsalis in China, its ecological and physiological mechanisms facilitating its invasion, and the progress made in understanding its major biological characteristics. Moreover, the key approaches for managing B. dorsalis that have been or are likely to be implemented in China are presented, including quarantine measures, monitoring procedures, physical controls, biological controls, the sterile insect technique, RNA interference, and CRISPR-Cas-9. Finally, some suggestions for future research directions are provided.

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Thermal tolerance of larvae of seven Chironomus species and up-regulation of heat shock protein-coding genes in Chironomus sulfurosus

Tettey,

Fujii,

Saito

et al. 2024

Limnology

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Global warming directly and indirectly affects the biology and ecology of aquatic insects. Chironomids are ubiquitous on the earth and are suitable for systematic analysis of the warming effect on insects. Here, we measured the survival rate for the fourth-instar larvae of seven Chironomus species at different temperatures. The mid-temperature for survival after 1-h temperature exposure to Chironomus sulfurosus larvae was the highest (43 °C) among seven Chironomus species. In addition, 14.5% of C. sulfurosus larvae survived at 40 °C from the first to the fourth instar for 14 days, whereas larvae of the other three species with relatively high thermal tolerance in the 1-h exposure test could not. These results indicate that only C. sulfurosus larvae are tolerant of 40 °C. We examined the expression levels of specific genes encoding four heat shock proteins and one heat shock protein cognate in C. sulfurosus larvae at various temperatures using quantitative real-time PCR. The expression levels of genes for hsc70, hsp67, hsp60, hsp27, and hsp23 genes were higher at 40 °C than those at 35 °C, at which 97% of larvae survived for 14 days. Therefore, in conjunction with the present phylogenetic analysis, the thermal tolerance of C. sulfurosus is associated with the up-regulated hsp and hsc genes, which may underlie a mechanism for the organism’s response to high temperatures.

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Transcriptome analysis of Chironomus sulfurosus larvae living in acidic environments: Insights into molecular mechanisms for acid tolerance

Cited by 5 publications

References 45 publications

Species-specific Microorganisms in Acid-tolerant <i>Chironomus</i> Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis

Species-specific Microorganisms in Acid-tolerant <i>Chironomus</i> Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis

Understanding the Invasion, Ecological Adaptations, and Management Strategies of Bactrocera dorsalis in China: A Review

Thermal tolerance of larvae of seven Chironomus species and up-regulation of heat shock protein-coding genes in Chironomus sulfurosus

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