Transcriptome Analysis of Energy Supply Process During Seed Germination in Phyllostachys edulis

Li, Juan; Li, Xiangyu; Bai, Yucong; Xie, Yali; Li, Long; Mu, Shaohua; Gao, Jian

doi:10.1007/s11105-023-01378-6

Cited by 2 publications

(2 citation statements)

References 56 publications

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“…Transport and degradation of stored lipids is an important physiological and biochemical metabolic process during plant seed germination, with LOX located on lipid body membranes being rate-limiting enzymes [66]. In this study, LOX1_5 was upregulated in stages B and C, while most LOX2S were upregulated in stages A/D, suggesting roles mainly in seed germination or regulation of linoleic acid oxidation.…”

Section: Lipids Release May Serve As a Potential Supplement In The Ca...mentioning

confidence: 56%

Combined Analysis of Untargeted Metabolomics and Transcriptomics Revealed Seed Germination and Seedling Establishment in Zelkova schneideriana

Yan,

Wei,

Yang

et al. 2024

Genes

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Zelkova schneideriana Hand.-Mazz is a valuable ornamental tree and timber source, whose seedling breeding and large-scale cultivation are restricted by low seed germination and seedling rates. The regulatory mechanisms underlying seed germination and seedling establishment in Z. schneideriana remain unknown. This study conducted metabolomic and transcriptomic analyses of seed germination and seedling establishment in Z. schneideriana. Regular expression of genes and metabolite levels has been observed in plant hormone signal transduction, starch and sucrose metabolism, linoleic acid metabolism, and phenylpropanoid biosynthesis. The reduction in abscisic acid during seed germination may lead to seed release from dormancy. After the seed is released from dormancy, the metabolic levels of auxin, cytokinins, brassinolide, and various sugars are elevated, and they are consumed in large quantities during the seedling establishment stage. Linoleic acid metabolism is gradually activated during seedling establishment. Transcriptome analysis showed that a large number of genes in different metabolic pathways are upregulated during plant establishment, and material metabolism may be accelerated during seedling establishment. Genes regulating carbohydrate metabolism are altered during seed germination and seedling establishment, which may have altered the efficiency of carbohydrate utilization. In addition, the syntheses of lignin monomers and cellulose have different characteristics at different stages. These results provide new insights into the complex mechanisms underlying seed germination and seedling establishment in Z. schneideriana and other woody plants.

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Section: Lipids Release May Serve As a Potential Supplement In The Ca...mentioning

confidence: 56%

Combined Analysis of Untargeted Metabolomics and Transcriptomics Revealed Seed Germination and Seedling Establishment in Zelkova schneideriana

Yan,

Wei,

Yang

et al. 2024

Genes

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“…The glycolytic pathway and citrate cycle in the respiration pathway supply energy during Phyllostachys edulis seed germination; moreover, the respiration intensity increased significantly with the 6-phosphofructokinase (PFK) enzyme peaking in stage II. [37]. At present, LOC_Os01g53680, one of the candidate genes for qSERg-1b, is annotated for the putative 6phosphofructokinase in rice; the energy supply is needed in stigma exsertion and the role of 6-phosphofructokinase in the stigma exsertion process could be probed in the future, which would be helpful in verifying the causal gene of qSERg-1b.…”

Section: Discussionmentioning

confidence: 99%

Fine-Mapping and Candidate Gene Analysis of qSERg-1b from O. glumaepatula to Improve Stigma Exsertion Rate in Rice

Cao,

Dan,

et al. 2024

Agronomy

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The stigma exsertion rate (SER) is one of the important factors affecting the seed production of hybrid rice. In the genus Oryza, wild rice species usually show higher SERs than cultivars. We previously identified one novel SER-QTL, qSERg-1b, from the single-segment substitution line (SSSL) SG22 of O. glumaepatula. In this study, qSERg-1b was fine-mapped by developing the secondary SSSLs (s-SSSLs) of SG22 and substitution mapping among the s-SSSLs. A total of 11 s-SSSLs were developed and used for qSERg-1b fine-mapping. As a result, qSERg-1b was narrowed down to the 122.59 kb physical region between InDel markers M01 and M49. There were 19 genes annotated in both O. glumaepatula and the HJX74 genomes at the qSERg-1b interval. Based on the genome re-sequencing of SG22 and HJX74, and the expression levels of the annotated functional genes, multiple nucleotide variations were found in LOC_Os01g53630, LOC_Os01g53680, and LOC_Os01g53710, including base substitutions and insertions/deletions of the genes, and they also showed significant differences in expression levels between SG22 and HJX74. Therefore, LOC_Os01g53630, LOC_Os01g53680, and LOC_Os01g53710 were selected as the most possible candidate genes of qSERg-1b for further validation. The above results laid an important foundation for qSERg-1b cloning and provided valuable resources for molecular breeding of SER in rice.

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Transcriptome Analysis of Energy Supply Process During Seed Germination in Phyllostachys edulis

Cited by 2 publications

References 56 publications

Combined Analysis of Untargeted Metabolomics and Transcriptomics Revealed Seed Germination and Seedling Establishment in Zelkova schneideriana

Combined Analysis of Untargeted Metabolomics and Transcriptomics Revealed Seed Germination and Seedling Establishment in Zelkova schneideriana

Fine-Mapping and Candidate Gene Analysis of qSERg-1b from O. glumaepatula to Improve Stigma Exsertion Rate in Rice

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