Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress

Song, Fuqiang; Li, Jize; Fan, Xiaoxu; Zhang, Quan; Chang, Wei; Fang, Yang; Geng, Gui

doi:10.1038/srep20245

Cited by 26 publications

(14 citation statements)

References 30 publications

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“…Analyzing the behavior of the extracellular laccase produced of P. tenuiculus MCA 11 considering the nitrogen and atrazine levels in the culture medium, the results suggest that the biosynthesis of this enzyme is induced by atrazine, in agreement with the previous ndings [36,37]. Besides this, the enzyme is repressed by nitrogen, while herbicide degradation rates were more in uenced by mycelium development than laccase levels.…”

Section: Previous Research Found That Atrazine Degradation By Phanerosupporting

confidence: 84%

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains 

Henn

Monteiro

Boscolo

et al. 2020

Preprint

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Background Atrazine is one of the most widespread chlorinated herbicides, leaving large bulks in soils and groundwater. The biodegradation of atrazine by bacteria is well described, but many aspects of the fungal metabolism of this compound remain unclear. Thus, we investigated the toxicity and degradation of atrazine by 13 rainforest basidiomycete strains. Results In liquid medium, Pluteus cubensis SXS320, Gloelophyllum striatum MCA7, and Agaricales MCA17 removed 30, 37, and 38%, respectively, of initial 25 mg L-1 of the herbicide within 20 days. Deficiency of nitrogen drove atrazine degradation by Pluteus cubensis SXS320; this strain removed 30% of atrazine within 20 days in a culture medium with 2.5 mM of N, raising three metabolites; in a medium with 25 mM of N, only 21% of initial atrazine were removed after 40 days, and two metabolites appeared in culture extracts. This is the first report of such different outcomes linked to nitrogen availability during the biodegradation of atrazine by basidiomycetes. The herbicide also induced synthesis and secretion of extracellular laccases by Datronia caperata MCA5, Pycnoporus sanguineus MCA16, and Polyporus tenuiculus MCA11. Laccase levels produced by of P. tenuiculus MCA11 were 13.3-fold superior in the contaminated medium than in control; the possible role of this enzyme on atrazine biodegradation was evaluated, considering the strong induction and the removal of 13.9% of the herbicide in vivo. Although 88% of initial laccase activity remained after 6 h, no evidence of in vitro degradation was observed, even though ABTS was present as mediator. Conclusions This study revealed a high potential for atrazine biodegradation among tropical basidiomycete strains. Further investigations, focusing on less explored ligninolytic enzymes and cell-bound mechanisms, could enlighten key aspects of the atrazine fungal metabolism and the role of the nitrogen in the process.

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Section: Previous Research Found That Atrazine Degradation By Phanerosupporting

confidence: 84%

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains 

Henn

Monteiro

Boscolo

et al. 2020

Preprint

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“…In study by Song et al analysis of transcriptome led to the determination of functional genes upregulated by stress caused by herbicideatrazine. Among these were genes encoding the zinc finger proteins, intracellular/ extracellular enzymes, structural proteins, anti-stress/anti-disease proteins, and electron transport-related proteins [5]. The results of another researchers showed regulatory mechanisms induced after the exposure to petroleum hydrocarbon stress in Z. mays [4].…”

Section: Transcriptomicmentioning

confidence: 98%

“…At the same time, one of the most frequent problems with research on soil microbial communities is that in many cases more than 99% of obtained microbial taxa are not yet cultured. Therefore, using NGS technologies can allow for significantly more thorough analysis of microbial composition and their activity while preserving environmental factors (both abiotic and biotic) that once shaped these microbial communities in situ [5]. In addition, to adequately probe the plant-associated microbial communities and examine plant-dependent habitats including plants rhizosphere, phyllosphere and endosphere, shotgun sequencing and amplicontargeting of genes can be also used as a promising tool [20].…”

Section: Metagenomic Approach To Enhance Phytoremediation Efficiencymentioning

confidence: 99%

“…Sedum alfredii cadmium 2016 [8] Populus nigra boron 2016 [9] Phytolacca americana cadmium 2018 [6] Medicago sativa atrazine 2016 [5] Landoltia punctata cadmium 2018 [34] Zea mays PAHs 2018 [4] Festuca arundinacea cadmium 2018 [15] Nicotiana tabacum thorium 2018 [30] Sorghum bicolor cadmium 2017 [14] 3.3. The use of omics for designing new transgenic species…”

Section: Species Contaminantmentioning

confidence: 99%

“…In comparison to bioremediation with bacteria and fungi, phytoremediation is visible and thus can be monitored and continuously controlled. Besides, plants can provide nutrients and stimulate rhizosphere bacteria which can also improve soil quality and aid the whole remediation process [5]. Finally, phytoremediation of degraded sites, especially after large-scale industrial activities, can provide a new habitat for wildlife including insects, birds and small mammals [6].…”

Section: Introductionmentioning

confidence: 99%

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Implementation of Omics Research to Enhance Phytoremediation Efficiency - a Review

Jaskulak

2018

Eng. Prot. Environ.

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Wdrożenie badań omicznych w celu zwiększenia efektywności fitoremediacji -przeglądPhytoremediation is recognized as a cost-effective and widely acceptable alternative to chemical and physical technologies of soil remediation but requires an overall longer time to achieve success. In the last decade, the vast progress in omics research had led to improvements in our understanding of plants metabolism under exposure to toxic substances and the interactions between microbial communities and plants. Omics research includes a number of disciplines aimed at explaining the biological and chemical principles of functioning a particular organism exposed to selected factors using modern methods of molecular biology (example: rt-qPCR -Quantitative polymerase chain reaction in real time). The names of individual branches of omics studies arise from a group of studied substances, example: transcriptomic refers to research related to changes occurring in the organism at the level of its transcriptome, and proteomics deals with the determination of complete information on the proteomic composition of a given sample. By merging available omics tools with new bioinformatic approaches, it is possible to understand and determinate the specific patterns of plants response to various stress factors. In this review, we provide an overview of how omics research including transcriptomic, proteomic, genomic and metagenomic approaches might be used to reduce the negative impact of toxic elements to plants growth and development in order to ultimately enhance the phytoremediation efficiency.

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Microbial community in the Leymus chinensis rhizosphere associates with its high production in mild saline–alkaline grassland

Guo,

Hao

et al. 2023

Land Degrad Dev

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Leymus chinensis is a dominant grass widely distributed in Central Asia, in both saline−alkaline and non‐saline−alkaline grasslands. However, the mechanisms underlying its saline−alkaline tolerance have not been explored from the perspective of rhizosphere microbial communities. We investigated L. chinensis rhizosphere microbial communities in both saline–alkaline and non‐saline–alkaline grasslands. It was found that the biomass of L. chinensis was remarkably higher in saline–alkaline than in non‐saline–alkaline habitat and that changes in the rhizosphere bacterial and arbuscular mycorrhizal fungi (AMF) communities had a greater effect on biomass than climate, soil moisture, nutrients, and salinity. We demonstrated that the bacteria and AMF in the rhizosphere of L. chinensis grown in saline–alkaline grassland differed from those in non‐saline–alkaline grassland, and the differences were associated with soil salinity or alkalinity. The co‐occurrence networks of bacteria and AMF and bacteria–AMF interaction networks in rhizosphere were complex and connected, with more nodes and edges and a higher average degree of nodes and modularity in the saline–alkaline grassland. More complex networks promoted plant tolerance and growth under salt–alkali stress. High number of microbial keystone taxa was related to the nutrient concentration in the soil and L. chinensis leaves, and more remarkable direct effects of the bacterial and AMF keystone communities on plant nutrient concentrations were demonstrated in saline–alkaline grassland. This indicates that bacterial and AMF communities in saline–alkaline grasslands are favorable for plant growth and nutrient uptake. This study provides an accurate and beneficial source of soil microbial assemblages for future plant–microbial joint remediation of the saline–alkaline ecosystems.

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Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress

Cited by 26 publications

References 30 publications

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Implementation of Omics Research to Enhance Phytoremediation Efficiency - a Review

Microbial community in the Leymus chinensis rhizosphere associates with its high production in mild saline–alkaline grassland

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Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress

Cited by 26 publications

References 30 publications

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains&nbsp;

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains&nbsp;

Implementation of Omics Research to Enhance Phytoremediation Efficiency - a Review

Microbial community in the Leymus chinensis rhizosphere associates with its high production in mild saline–alkaline grassland

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Resources

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Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains