Transcriptome analysis of Hevea brasiliensis in response to exogenous methyl jasmonate provides novel insights into regulation of jasmonate-elicited rubber biosynthesis

Liu, Jinping; Hu, Jin; Liu, Yan‐Hui; Yang, Cuiping; Zhuang, Yufen; Guo, Xiuli; Li, Yijian; Zhang, Liangsheng

doi:10.1007/s12298-018-0529-0

Cited by 22 publications

(16 citation statements)

References 83 publications

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“…wenyujin (Figs 3 and 4 ). One major regulatory mechanism of MeJA-mediated secondary metabolite production in many plants, is to control related genes in biosynthesis pathway [ 43 , 44 ]. Consistent with this regulatory mechanism, related genes in JA biosynthesis, JA signaling transduction, and terpenes biosynthesis pathway in C .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals regulation mechanism of methyl jasmonate-induced terpenes biosynthesis in Curcuma wenyujin

Wei

Lan²,

Liu³

et al. 2022

PLoS ONE

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Curcuma wenyujin is the source plant of three traditional Chinese medicines, which have been widely used in clinical treatment over 1000 years. The content of terpenes, the major medicinal active ingredients, is relatively low in this plant. Studies have shown that MeJA can promote terpenes biosynthesis in plants. However, the mechanism underlying the effect of MeJA in C. wenyujin remains unclear. In this work, the transcriptome of C. wenyujin leaves with MeJA treatment was analyzed to elucidate the regulation mechanism of MeJA-mediated terpene biosynthesis. Based on the RNA-seq data, 7,246 unigenes were differentially expressed with MeJA treatment. Expression pattern clustering of DEGs revealed that unigenes, related to JA biosynthesis and signal transduction, responded to exogenous MeJA stimulation on the early stage and maintained throughout the process. Subsequently, unigenes related to terpene biosynthesis pathway showed a significant up-regulation with 6 h treatment. The analysis results suggested that MeJA induced the expression of JA biosynthesis genes (such as LOXs, AOSs, AOCs, OPRs, and MFPs) and JA signal transduction core genes (JAZs and MYCs) to activate JA signaling pathway. Meanwhile, downstream JA-responsive genes presented up-regulated expression levels such as AACT, HMGSs, HMGRs, DXSs, DXRs, MCTs, HDSs, and HDRs, thus promoting terpenes biosynthesis. The transcriptional expressions of these genes were validated by qRT-PCR. In addition, six CwTPS genes in response to MeJA were identified. With MeJA treatment, the expression levels of CwTPSs were increased as well as those of the transcription factors MYB, NAC, bZIP, WRKY, AP2/ERF, and HLH. These TFs might potentially regulate terpenes biosynthesis. These results provide insights for regulation mechanism of terpenes biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals regulation mechanism of methyl jasmonate-induced terpenes biosynthesis in Curcuma wenyujin

Wei

Lan²,

Liu³

et al. 2022

PLoS ONE

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“…In rubber trees (Hevea brasiliensis), natural rubber synthesis is the main metabolic activity in laticifers which are highly specialized cells that directly influence the yield of rubber [ 19]. Most studies in molecular biology, especially those using gene expression profiling, have paid close attention to rubber biosynthesis in laticifers [20][21][22][23][24][25]. Previously, we have evaluated the expression stability of 22 reference genes across abundant laticifer samples, including latex regenerations, hormone treatments, tappings, individual trees and genotypes of Hevea [ 26,27].…”

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confidence: 99%

Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf

Long

Kav

et al. 2020

Mol Biol Rep

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Backgroud Gene expression profiling is increasingly applied to investigate molecular mechanisms for which, normalization with suitable reference genes is critical. Previously we have reported several suitable reference genes for laticifer samples from rubber, however, little is known about reference genes in leaf. Results The main objective of this current study was to identify some reference genes with stable expression patterns in leaf at various developmental stages, as well as during abiotic (temperature extremes) and biotic stresses. Gene expression profiling experiments in rubber tree leaf identified the ubiquitinproteasome system as having excellent potential as reference genes. Among a total of 30 tested genes investigated, 24 new (including 11 genes involved in the ubiquitin-proteasome system), 4 previously identified and 2 specific genes, were further evaluated using quantitative real-time PCR. Our results indicated that the new genes had better stability of expression when compared with others. For instance, an ubiquitin conjugating enzyme (RG0099) and three ubiquitin-protein ligases (RG0928, RG2190 and RG0118) expressed stably in all samples, and were confirmed to be suitable reference genes in rubber tree leaf in four different conditions. Finally, we suggest that using more than one reference gene may be appropriate in gene expression studies when employing different software to normalize gene expression data. Conclusion Our findings have significant implications for the reliability of data obtained from genomics studies in rubber tree and perhaps in other species. Background Gene expression profiling studies are widely used to investigate plant processes, including metabolic pathways, including regulatory networks that are crucial for plant growth & development as well as in stress responses. These types of genomics-based investigation necessitate the use of appropriate reference genes to normalize gene expression data. Incorrect choice of reference genes in techniques

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“…Previous studies demonstrated that the application of MeJA induced the accumulation of different secondary metabolites in several plant species, including Lycoris chinensis ( Mu et al, 2009 ), Dendrobium officinale ( Jiao et al, 2018 ), Physalis angulata L. ( Zhan et al, 2020 ), Isatis tinctoria L. ( Gai et al, 2019 ), and Vitis labrusca L. ( Moro et al, 2020 ). One major regulatory mechanism of MeJA-mediated secondary metabolite production in many plants is through the control of pathway related genes ( Liu et al, 2018 ; Li et al, 2019a ; Li W. et al, 2020 ). For example, the expression of some genes in terpenoid indole alkaloid (TIAs) pathways was induced by MeJA treatment in Catharanthus roseus ( Pan et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…Methyl jasmonate treatment not only induces the expression of genes involved in JA biosynthetic pathways, but it also can induce JA signaling pathways ( Liu et al, 2018 ; Li W. et al, 2020 ). Similar results were observed in the present study, wherein RNA-seq and metabolite profiling suggested that the expression of putative JA biosynthesis genes ( AOC and OPR ) and JA signaling pathway genes ( JAR and MYC ) were upregulated.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveals That Exogenous Methyl Jasmonate Regulates Galanthamine Biosynthesis in Lycoris longituba Seedlings

Zheng

et al. 2021

Front. Plant Sci.

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The Amaryllidaceae alkaloid galanthamine (Gal) in Lycoris longituba is a secondary metabolite that has been used to treat Alzheimer’s disease. Plant secondary metabolism is affected by methyl jasmonate (MeJA) exposure, although the regulatory mechanisms of MeJA on L. longituba seedlings remains largely unknown. In the present study, 75, 150, and 300 μM MeJA were used as treatments on L. longituba seedlings for 7, 14, 21, and 28 days, while 0 μM MeJA was used as the control (MJ-0). The effect of exogenous MeJA on Gal synthesis in L. longituba was then investigated using transcriptomic sequencing and metabolite profiling via GC-MS and LC-MS analysis. Galanthamine (Gal), lycorine (Lyc), and lycoramine (Lycm) abundances were 2. 71-, 2. 01-, and 2.85-fold higher in 75 μM MeJA (MJ-75) treatment plants compared to MJ-0 treatment plants after 7 days of cultivation. Transcriptomic analysis further showed that MJ-75 treatment significantly induced the expression of norbelladine synthase (NBS) and norbelladine 4′-O-methyltransferase (OMT), which are involved in the Gal biosynthesis pathway. In addition, increased expression was observed in MJ-75 treatment plants for genes in the JA synthesis and JA signaling pathways including those of allene oxide cyclase (AOC), 12-oxo-phytodienoic acid reductase (OPR), jasmonic acid amino acid synthase (JAR), and transcription factor MYC. The L. longituba tyrosine decarboxylase (LlTYDC) enzyme was identified and proposed to be involved in the Gal biosynthetic pathway. Metabolomics results demonstrated that the accumulation of Amaryllidaceae alkaloids, and especially alkaloids in the Gal biosynthesis pathway, could be induced by MJ-75 treatment. Interestingly, metabolites in the JA synthesis pathway were also affected by MeJA treatment. Overall, this multi-omics study suggests that both the JA synthesis/JA signaling and Gal biosynthesis pathways were affected by exogenous MeJA treatment. This comprehensive study of gene expression and metabolite contents can help us better understand the molecular mechanisms underlying MeJA-mediated Gal biosynthesis in L. longituba.

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Transcriptome analysis of Hevea brasiliensis in response to exogenous methyl jasmonate provides novel insights into regulation of jasmonate-elicited rubber biosynthesis

Cited by 22 publications

References 83 publications

Transcriptome analysis reveals regulation mechanism of methyl jasmonate-induced terpenes biosynthesis in Curcuma wenyujin

Transcriptome analysis reveals regulation mechanism of methyl jasmonate-induced terpenes biosynthesis in Curcuma wenyujin

Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf

Transcriptomic and Metabolomic Analyses Reveals That Exogenous Methyl Jasmonate Regulates Galanthamine Biosynthesis in Lycoris longituba Seedlings

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