Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus

Ammour, Yulia; Susova, Olga Yu.; Krasnov, George S.; Nikolaeva, E. Yu.; Varachev, Vyacheslav; Schetinina, Yulia; Gavrilova, Marina L.; Митрофанов, А. А.; Poletaeva, Anna; Bekyashev, A.; Файзулоев, Е. Б.; Zverev, V. V.; Svitich, Oxana; Nasedkina, T. V.

doi:10.3390/v14112433

Cited by 9 publications

(1 citation statement)

References 48 publications

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“…The cytotoxic activity of the compounds was evaluated by the MTT method on six cell lines and one primary human tumor culture to determine the semi-inhibitory concentration ( IC 50) for non-small cell lung cancer A549, colon cancer HCT-116, hepatocarcinoma Huh7, pancreatic carcinoma PANC-1, breast cancer SKBR3, ovarian cancer SKOV3, and a primary culture of human glioblastoma Gbl13n we had obtained earlier [ 14 , 15 ]. The data presented in Fig.…”

Section: Biological Activitymentioning

confidence: 99%

Dimeric Bis-Benzimidazole-Pyrroles DB2Py(n) – AT-Site-Specific Ligands: Synthesis, Physicochemical Analysis, and Biological Activity

Susova,

Каrshieva,

Кostyukov

et al. 2024

Acta Naturae

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Its broad spectrum of biological activity makes benzimidazole a fundamental pharmacophore in pharmaceutics. The paper describes newly synthesized AT-specific fluorescent bis-benzimidazole molecules DB2Py(n) that contain a pyrrolcarboxamide fragment of the antibiotic drug netropsin. Physico-chemical methods using absorption, fluorescence, and circular dichroism spectra have shown the ability of bis-benzimidazole-pyrroles to form complexes with DNA. The new DB2Py(n) series have turned out to be more toxic to human tumor lines and less vulnerable to non-tumor cell lines. Bis-benzimidazole-pyrroles penetrated the cell nucleus, affected the cell-cycle synthesis (S) phase, and inhibited eukaryotic topoisomerase I in a cell-free model at low concentrations. A real-time tumor cell proliferation test confirmed the molecule’s enhanced toxic properties upon dimerization. Preliminary cytotoxicity data for the bis-benzimidazole-pyrroles tested in a cell model with a MDR phenotype showed that monomeric compounds can overcome MDR, while dimerization weakens this ability to its intermediate values as compared to doxorubicin. In this respect, the newly synthesized cytotoxic structures seem promising for further, in-depth study of their properties and action mechanism in relation to human tumor cells, as well as for designing new AT-specific ligands.

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Section: Biological Activitymentioning

confidence: 99%

Dimeric Bis-Benzimidazole-Pyrroles DB2Py(n) – AT-Site-Specific Ligands: Synthesis, Physicochemical Analysis, and Biological Activity

Susova,

Каrshieva,

Кostyukov

et al. 2024

Acta Naturae

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Sensitivity of Primary Human Glioblastoma Cell Lines to the Mumps Virus Vaccine Strain

Nikolaeva,

Zhelayeva,

Susova

et al. 2024

Mol Biol

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[Short Communication] Measles: 1963-2023, Immunology of a Morbillivirus

Brown

2023

Preprint

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Measles is a virus, abbreviated to MeV, that has long been known to be causal in infant disease and affect infant mortality, remaining a public health issue of priority. The causal virion is defined biologically within the Family _Paraxmyxoviridae_, Genus _Morbillivirus_ and Species _MeaslesMorbillivirus. _Similar to other viral infections, MeV is an airborne infection with the virion particle composed of a negative (-ve) sense single-stranded (ss) ribonucleic acid (RNA) genome code, around 15-16kb in size, encoding for eight predominant proteins. The first isolation of MeV occurred in 1954, known as the “Edmonston strain”. A team at Boston Children’s Hospital comprised of John Franklin Enders and others who isolated MeV from a 13-year-old serum sample. Alongside Samuel Katz and notably Maurice Hilleman, this led to the development of the first live attenuated vaccine, when in 1971, the first trivalent mumps, measles and rubella (MMR) vaccine was licensed for use in immunisation programmes in the United States of America (USA). Shortly after, in 1980, the eradication of Smallpox was confirmed by the World Health Organisation (WHO), which had been the predominant debilitating pathogen of the 20th century. Measles was then considered to be the cause of 2.6 million deaths each year. Around 1986, the MeV haemagglutinin (H) protein was crystallised _in vitro_. The introduction of MMR immunisation previously and after reduced mortality to around 110,000 annually. The rates of MeV disease since 2017 have been rising of a pathogen that is largely preventable through immunisation programs that evoke immune system responses. Smallpox (VARV) and the Rinderpest virus (RPV), a member of the same Morbillivirus genus as MeV, remain the only other animal pathogens eradicated. The lack of antigenic variation of the MeV is suggestive that MeV remains the third pathogen to potentially be eradicated. Here is a discussion of contextual Measles immunological characteristics to elucidate this further.

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Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus

Cited by 9 publications

References 48 publications

Dimeric Bis-Benzimidazole-Pyrroles DB2Py(n) – AT-Site-Specific Ligands: Synthesis, Physicochemical Analysis, and Biological Activity

Dimeric Bis-Benzimidazole-Pyrroles DB2Py(n) – AT-Site-Specific Ligands: Synthesis, Physicochemical Analysis, and Biological Activity

Sensitivity of Primary Human Glioblastoma Cell Lines to the Mumps Virus Vaccine Strain

[Short Communication] Measles: 1963-2023, Immunology of a Morbillivirus

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