Transcriptome analysis ofCampylobacter jejunipolyphosphate kinase (ppk1andppk2) mutants

Chandrashekhar, Kshipra; Kassem, Issmat I.; Nislow, Corey; Gangaiah, Dharanesh; Candelero-Rueda, Rosario A.; Rajashekara, Gireesh

doi:10.1080/21505594.2015.1104449

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…4 and Supplementary Table 6). PPKs have been related to motility, quorum sensing, biofilm formation, and virulence of P. aeruginosa and regulation of stress response in Campylobacter jejuni [59][60][61][62][63]. We hypothesize that pap might play a similar role in CHA0 insect pathogenesis.…”

Section: Bpmentioning

confidence: 92%

Transcriptome plasticity underlying plant root colonization and insect invasion by Pseudomonas protegens

et al. 2020

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Pseudomonas protegens shows a high degree of lifestyle plasticity since it can establish both plant-beneficial and insect-pathogenic interactions. While P. protegens protects plants against soilborne pathogens, it can also invade insects when orally ingested leading to the death of susceptible pest insects. The mechanism whereby pseudomonads effectively switch between lifestyles, plant-beneficial or insecticidal, and the specific factors enabling plant or insect colonization are poorly understood. We generated a large-scale transcriptomics dataset of the model P. protegens strain CHA0 which includes data from the colonization of wheat roots, the gut of Plutella xylostella after oral uptake and the Galleria mellonella hemolymph after injection. We identified extensive plasticity in transcriptomic profiles depending on the environment and specific factors associated to different hosts or different stages of insect infection. Specifically, motor-activity and Reb toxin-related genes were highly expressed on wheat roots but showed low expression within insects, while certain antimicrobial compounds (pyoluteorin), exoenzymes (a chitinase and a polyphosphate kinase), and a transposase exhibited insect-specific expression. We further identified two-partner secretion systems as novel factors contributing to pest insect invasion. Finally, we use genus-wide comparative genomics to retrace the evolutionary origins of cross-kingdom colonization.

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Section: Bpmentioning

confidence: 92%

Transcriptome plasticity underlying plant root colonization and insect invasion by Pseudomonas protegens

et al. 2020

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“…is not feasible in these pathogens; previous attempts at computational predictions have also failed (18). However, recent work in Campylobacter has demonstrated that RNAseq can be useful for analyzing transcriptomic differences between wild-type and mutant strains following inactivation of protein-coding genes (42,43). Indeed, over the past several years, RNAseq has become the method of choice to analyze the transcriptomes of bacteria under various conditions, as it allows evaluation of the entire transcriptome rather than only previously annotated regions as with older technologies such as microarrays (44)(45)(46).…”

Section: Figmentioning

confidence: 99%

Small Noncoding RNA CjNC110 Influences Motility, Autoagglutination, AI-2 Localization, Hydrogen Peroxide Sensitivity, and Chicken Colonization in Campylobacter jejuni

et al. 2020

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Small noncoding RNAs (ncRNAs) are involved in many important physiological functions in pathogenic microorganisms. Previous studies have identified the presence of noncoding RNAs in the major zoonotic pathogen Campylobacter jejuni; however, few have been functionally characterized to date. CjNC110 is a conserved ncRNA in C. jejuni, located downstream of the luxS gene, which is responsible for the production of the quorum sensing molecule autoinducer-2 (AI-2). In this study, we utilized strand specific high-throughput RNAseq to identify potential targets or interactive partners of CjNC110 in a sheep abortion clone of C. jejuni. These data were then utilized to focus further phenotypic evaluation of the role of CjNC110 in motility, autoagglutination, quorum sensing, hydrogen peroxide sensitivity, and chicken colonization in C. jejuni. Inactivation of the CjNC110 ncRNA led to a statistically significant decrease in autoagglutination ability as well as increased motility and hydrogen peroxide sensitivity compared to the wild-type. Extracellular AI-2 detection was decreased in ΔCjNC110; however, intracellular AI-2 accumulation was significantly increased, suggesting a key role of CjNC110 in modulating the transport of AI-2. Notably, ΔCjNC110 also showed a decreased ability to colonize chickens. Complementation of CjNC110 restored all phenotypic changes back to wild-type levels. The collective results of the phenotypic and transcriptomic changes observed in our data provide valuable insights into the pathobiology of C. jejuni sheep abortion clone and strongly suggest that CjNC110 plays an important role in the regulation of energy taxis, flagellar glycosylation, cellular communication via quorum sensing, oxidative stress tolerance, and chicken colonization in this important zoonotic pathogen.

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“…C. jejuni bacteria pellets were resuspended in RNAprotect Bacteria Reagent (Qiagen) to preserve them. For RNA preparation, bacteria pellets were resuspended in Qiagen RNeasy lysis buffer containing β-mercaptoethanol and shocked in a vortex for 30 s. The RNA was further purified using the Qiagen RNeasy Plus mini kit as described previously and in the manufacturer’s instructions ( Chandrashekhar et al, 2015 ). The absence of DNA was confirmed by PCR amplification using Campylobacter -specific 16S rRNA primers, and total RNA was quantified in a NanoDrop 1000 device (Thermo Scientific, Germany).…”

Section: Methodsmentioning

confidence: 99%

The Novel Protein Cj0371 Inhibits Chemotaxis of Campylobacter jejuni

Kong

Tang

et al. 2018

Front. Microbiol.

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cj0371 is a novel gene that is associated with Campylobacter jejuni virulence, and an isogenic mutant of cj0371 showed hyper chemotaxis and motility. Chemotactic motility is an important virulence factor and is involved in C. jejuni pathogenesis. Campylobacter sp. has specific variations of the common chemotaxis components, including histidine autokinase CheA, coupling scaffold protein CheV, chemotaxis response regulator protein CheY and several chemoreceptor proteins. In this study, we used immunoprecipitation combined with LC-MS/MS analyses to screen six chemotaxis pathway proteins that potentially interact with the putative protein Cj0371. qRT-PCR was used to quantitatively analyze the expression of these chemotaxis genes and basic flagella genes. The results showed that the expression of cheV, cj1110c, and cj0262c was significantly up-regulated, and four flagella genes also had up-regulated expression in the cj0371 mutant. GST pull-down analyses found that Cj0371 interacted with the receiver domain of the CheV protein. Enzyme-coupled spectrophotometric assays showed that the ATPase activity of CheA was higher when Cj0371 was not present in the chemotaxis reaction medium. Therefore, we concludes that cj0371 has a negative influence on C. jejuni chemotaxis, which may occur by adjusting the receiver domain of CheV to influence chemotaxis. This paper provides a new component in the chemotaxis pathway of C. jejuni for the first time and highlight the complexity of this remarkable pathway.

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Transcriptome analysis ofCampylobacter jejunipolyphosphate kinase (ppk1andppk2) mutants

Cited by 7 publications

References 37 publications

Transcriptome plasticity underlying plant root colonization and insect invasion by Pseudomonas protegens

Transcriptome plasticity underlying plant root colonization and insect invasion by Pseudomonas protegens

Small Noncoding RNA CjNC110 Influences Motility, Autoagglutination, AI-2 Localization, Hydrogen Peroxide Sensitivity, and Chicken Colonization in Campylobacter jejuni

The Novel Protein Cj0371 Inhibits Chemotaxis of Campylobacter jejuni

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