Transcriptome Analysis of Long-lived Drosophila melanogaster E(z) Mutants Sheds Light on the Molecular Mechanisms of Longevity

Moskalev, Alexey; Shaposhnikov, Mikhail; Zemskaya, Nadezhda; Koval, Liubov; Schegoleva, Eugenia V.; Guvatova, Zulfiya G.; Krasnov, George S.; Solovev, Ilya; Sheptyakov, Maksim; Zhavoronkov, Alex; Kudryavtseva, Anna V.

doi:10.1038/s41598-019-45714-x

Cited by 37 publications

(27 citation statements)

References 74 publications

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“…134 Intriguingly, knocking down LMNB in young adult or larval fat body resulted in reduced heterochromatin, and an increase in retrotransposon expression and DNA damage. 132,133 Collectively, these studies provide evidence of the importance of GWAS on age at menarche [302] of specific genomic areas with aging-associated augmentation of H3K27me3, [149][150][151] and this increase is particularly high in the head. 149 Loss-of-function mutations in the genes encoding specific PRC1 and PRC2 components suppressed the elevation of H3K27me3 during aging and improved lifespan and healthspan.…”

Section: Drosophilamentioning

confidence: 81%

Aging mechanisms—A perspective mostly from Drosophila

Tsurumi

2020

Advanced Genetics

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A mechanistic understanding of the natural aging process, which is distinct from aging-related disease mechanisms, is essential for developing interventions to extend lifespan or healthspan. Here, we discuss current trends in aging research and address conceptual and experimental challenges in the field. We examine various molecular markers implicated in aging with an emphasis on the role of heterochromatin and epigenetic changes. Studies in model organisms have been advantageous in elucidating conserved genetic and epigenetic mechanisms and assessing interventions that affect aging. We highlight the use of Drosophila, which allows controlled studies for evaluating genetic and environmental contributors to aging conveniently. Finally, we propose the use of novel methodologies and future strategies using Drosophila in aging research.

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Section: Drosophilamentioning

confidence: 81%

Aging mechanisms—A perspective mostly from Drosophila

Tsurumi

2020

Advanced Genetics

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“…Studies on model organisms have highlighted that ageing is characterized by many alterations at molecular, cellular and tissue level [3]. Studies of the ageing transcriptome have been performed in species including C. elegans [4], ies [5], rodents [6] and humans [7]. This approach has identi ed various signatures found to occur repeatedly across different tissues and organisms.…”

Section: Introductionmentioning

confidence: 99%

Age Related Changes in the Circulating Leukocyte Transcriptome in Dairy Cows

Buggiotti

Cheng

Salavati

et al. 2020

Preprint

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Background: Previous studies of ageing have identified many pathways which are consistently altered in humans and model organisms. Dairy cows are often culled at quite young ages due to an inability to cope adequately with metabolic and infectious diseases, resulting in reduced milk production and infertility. Improved longevity is therefore a desirable trait which would benefit both farmers and their cows. This study analysed the transcriptome derived from RNA-seq data of leukocytes obtained from Holstein cows in early lactation with respect to lactation number. Results: Samples were divided into three age groups for analysis: i) primiparous (PP, n = 53), ii) multiparous in lactations 2-3 (MP 2-3, n = 121), and iii) MP in lactations 4-7 (MP>3, n = 55). Leukocyte expression was compared between PP vs MP>3 cows with MP 2-3 as background using DESeq2 followed by weighted gene co-expression network analysis (WGCNA). Seven modules were significantly correlated (r ≥ 0.25) to the trait age. Genes from the modules which were more highly expressed in either the PP or MP>3 cows were pooled, and the gene lists subjected to David functional annotation cluster analysis. The top three clusters from modules more highly expressed in the PP cows all involved regulation of gene transcription, particularly zinc fingers. Another cluster included genes encoding enzymes in the mitochondrial beta-oxidation pathway. Top clusters up-regulated in MP>3 cows included the terms Glycolysis/Gluconeogenesis, C-type lectin, and Immunity. Differentially expressed candidate genes for ageing previously identified in the human blood transcriptome up-regulated in PP cows were mainly associated with T-cell function (CCR7, CD27, IL7R, CAMK4, CD28), mitochondrial ribosomal proteins (MRPS27, MRPS9, MRPS31), and DNA replication and repair (WRN). Those up-regulated in MP>3 cows encoded immune defence proteins (LYZ, CTSZ, SREBF1, GRN, ANXA5, ADARB1). Conclusions: Genes and pathways associated with age in cows were identified for the first time to date, and we found that many were comparable to those known to be associated with ageing in humans and model organisms. We also detected changes in energy utilization and immune responses in leukocytes with age.

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“…Previous studies founded a positive association between stress resistance and extended lifespan or aging in D. melanogaster [34,43,44]. In this species, the ROS defences are mediated by both immune and antioxidant response pathways.…”

Section: Suzukii Genotypes Vary In Lifespan and Response To Oxidatmentioning

confidence: 97%

Phenotypic and transcriptomic responses to stress differ according to population geography in an invasive species

St.Pierre

Jaquet

Picarle

et al. 2020

Preprint

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BackgroundAdaptation to rapid environmental changes must occur within a short time scale. In this context, studies of invasive species may provide insights into the underlying mechanisms of rapid adaptation as these species have repeatedly encountered and successfully adapted to novel environmental conditions. Here we investigated how invasive and non-invasive populations of D. suzukii deal with an oxidative stress at both the phenotypic and molecular level. We also investigated the impact of transposable element insertions on the differential gene expression between genotypes in response to oxidative stress.ResultsInvasive populations lived longer in the untreated condition than non-invasive Japanese populations. As expected, lifespan was greatly reduced following exposure to paraquat, but this reduction varied among genotypes (a genotype by environment interaction, GEI) with invasive genotypes appearing more affected by exposure than non-invasive genotypes. We also performed transcriptomic sequencing of selected genotypes upon and without paraquat and detected a large number of genes differentially expressed, distinguishing the genotypes in the untreated environment. While a small core set of genes were differentially expressed by all genotypes following paraquat exposure, much of the response of each population was unique. Interestingly, we identified a set of genes presenting genotype by environment interaction (GEI). Many of these differences may reflect signatures of history of past adaptation. Transposable elements (TEs) were not activated after oxidative stress and differentially expressed (DE) genes were significantly depleted of TEs.ConclusionIn the decade since the invasion from the south of Asia, invasive populations of D. suzukii have diverged from populations in the native area regarding their genetic response to oxidative stress. This suggests that such transcriptomic changes could be involved in the rapid adaptation to local environments.

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Transcriptome Analysis of Long-lived Drosophila melanogaster E(z) Mutants Sheds Light on the Molecular Mechanisms of Longevity

Cited by 37 publications

References 74 publications

Aging mechanisms—A perspective mostly from Drosophila

Aging mechanisms—A perspective mostly from Drosophila

Age Related Changes in the Circulating Leukocyte Transcriptome in Dairy Cows

Phenotypic and transcriptomic responses to stress differ according to population geography in an invasive species

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