Transcriptome analysis of megalurothrips usitatus (Bagnall) identifies olfactory genes with ligands binding characteristics of MusiOBP1 and MusiCSP1

Li, Zhaoyang; Chen, Weiyi; Wang, Xiaoshuang; Sang, Wen; Pan, Huipeng; Ali, Shaukat; Tang, Liangde; Wu, Jing

doi:10.3389/fphys.2022.978534

Cited by 4 publications

(5 citation statements)

References 59 publications

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“…For the newly identified OBPs, both qRT-PCR results and FPKM values supported Esig-GOBP14, EsigGOBP13, EsigOBP14, and EsigOBP17 as the fourth highest expression OBPs in adult antennae, suggesting their potential roles as key proteins in binding plant volatiles. This pattern is similar to MusiOBP1, which was the highest expressed in primordial females in Megalurothrips usitatus [34]. In addition, OBPs often exhibited a male antennae-biased expression pattern, as observed in examples like OlotOBP1, OlotOBP4, and OlotOBP6 [31].…”

Section: Discussionsupporting

confidence: 60%

“…The FPKM values indicate that EsigCSP14 is 50-to 10,000-fold more highly expressed than other EsigCSPs, suggesting that EsigCSP14 is the main and key CSP in adults and is not expressed in larvae. It is worth noting that the expression of CSPs can vary across different developmental stages; for example, GcanCSP4 showed the highest expression in G. cantor female antennae at 12 days [27], and MusiCSP1 was most highly expressed in M. usitatus primordial pupae [34]. Additionally, CSPs can be expressed in non-olfactory tissues and show division of labor biased in society insects.…”

Section: Discussionmentioning

confidence: 99%

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A Study of Adult Olfactory Proteins of Primitive Ghost Moth, Endoclita signifer (Lepidoptera, Hepialidae)

Xiao,

Lu,

Yang

et al. 2023

Life

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Endoclita signifer is a prominent wood-boring insect species in eucalyptus plantations in Guangxi, China, causing significant ecological and economic damage. A novel approach to controlling the challenging wood-boring pest involves disrupting the olfactory communication between insects and the volatile compounds emitted by plants. To identify the olfactory proteins contributing to host selection based on 11 GC-EAD-active volatiles from eucalyptus leaves and to discover the highly expressed olfactory proteins, we conducted a study on the antennal transcriptomes of adult E. signifer and screened key olfactory proteins in the antennae. We identified a total of 69 olfactory proteins. When compared to the larval transcriptomes, the antennal transcriptome of adult E. signifer revealed the presence of 17 new odorant-binding proteins (OBPs), including 2 pheromone-binding proteins (PBPs), 7 previously unreported chemosensory proteins (CSPs), 17 new odorant receptors (ORs), 4 new gustatory receptors (GRs), 11 novel ionotropic receptors (IRs), and 2 sensory neuron membrane proteins (SNMPs). Through the phylogenetic tree of OBPs and ORs, we identified EsigPBP2 and EsigPBP3 as two of the three PBPs, designated EsigOR13 as EsigOrco, and recognized EsigOR10 and EsigOR22 as the newly discovered EsigPRs in E. signifer. In the adult antennae, the expression levels of EsigGOBP14, EsigGOBP13, EsigOBP14, EsigOBP17, EsigCSP14, and EsigOR16 were notably high, indicating that these proteins could be pivotal in binding to plant volatiles.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

A Study of Adult Olfactory Proteins of Primitive Ghost Moth, Endoclita signifer (Lepidoptera, Hepialidae)

Xiao,

Lu,

Yang

et al. 2023

Life

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“…Megalurothrips usitatus (Bagnall, commonly known as bean flower thrips) is a smallsized (~2 mm) destructive pest of vegetable crops, particularly legumes such as cowpea, in Asia [1]. The entire life cycle of the host plant, especially the flowering stage, is affected by M. usitatus feeding on flowers, leaves and fruits, resulting in the necrosis and premature abortion of buds and flowers [2].…”

Section: Introductionmentioning

confidence: 99%

“…The first species is F. occidentalis, which was assembled to a contig-level genome using Illumina short reads [10], while T. palmi is the first and only species with a chromosome-level genome assembled using PacBio long reads combined with a high-throughput chromosome conformation capture (Hi-C) strategy [11]. Although there have been some genetic analyses of M. usitatus, such as transcriptome and mitochondrial genome analyses [1,12], a highquality genome assembly of this species is needed to facilitate future studies on the genetics, diversity, adaptation and evolution of thrips.…”

Section: Introductionmentioning

confidence: 99%

The Chromosome-Level Genome Assembly of Bean Blossom Thrips (Megalurothrips usitatus) Reveals an Expansion of Protein Digestion-Related Genes in Adaption to High-Protein Host Plants

Zhang

Bao

Chen

et al. 2023

IJMS

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Megalurothrips usitatus (Bagnall) is a destructive pest of legumes, such as cowpea. The biology, population dynamics and control strategies of this pest have been well studied. However, the lack of a high-quality reference genome for M. usitatus has hindered the understanding of key biological questions, such as the mechanism of adaptation to feed preferentially on high-protein host plants and the resistance to proteinase inhibitors (PIs). In this study, we generated a high-resolution chromosome-level reference genome assembly (247.82 Mb, 16 chromosomes) of M. usitatus by combining Oxford Nanopore Technologies (ONT) and Hi-C sequencing. The genome assembly showed higher proportions of GC and repeat content compared to other Thripinae species. Genome annotation revealed 18,624 protein-coding genes, including 4613 paralogs that were preferentially located in TE-rich regions. GO and KEGG enrichment analyses of the paralogs revealed significant enrichment in digestion-related genes. Genome-wide identification uncovered 506 putative digestion-related enzymes; of those, proteases, especially their subgroup serine proteases (SPs), are significantly enriched in paralogs. We hypothesized that the diversity and expansion of the digestion-related genes, especially SPs, could be driven by mobile elements (TEs), which promote the adaptive evolution of M. usitatus to high-protein host plants with high serine protease inhibitors (SPIs). The current study provides a valuable genomic resource for understanding the genetic variation among different pest species adapting to different plant hosts.

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“…The expression of OBP and CSP genes varies with the developmental stages (egg, nymph, pupa, adult) of different insect species 12 , 13 . Understanding these variations is important because it can provide insights into how insects perceive and respond to their environment at different life stages.…”

Section: Introductionmentioning

confidence: 99%

Variations in the expression of odorant binding and chemosensory proteins in the developmental stages of whitefly Bemisia tabaci Asia II-1

Gouda,

Subramanian

2024

Sci Rep

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The cotton whitefly, Bemisia tabaci, is considered as a species complex with 46 cryptic species, with Asia II-1 being predominant in Asia. This study addresses a significant knowledge gap in the characterization of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in Asia II-1. We explored the expression patterns of OBPs and CSPs throughout their developmental stages and compared the motif patterns of these proteins. Significant differences in expression patterns were observed for the 14 OBPs and 14 CSPs of B. tabaci Asia II-1, with OBP8 and CSP4 showing higher expression across the developmental stages. Phylogenetic analysis reveals that OBP8 and CSP4 form distinct clades, with OBP8 appearing to be an ancestral gene, giving rise to the evolution of other odorant-binding proteins in B. tabaci. The genomic distribution of OBPs and CSPs highlights gene clustering on the chromosomes, suggesting functional conservation and evolutionary events following the birth-and-death model. Molecular docking studies indicate strong binding affinities of OBP8 and CSP4 with various odour compounds like β-caryophyllene, α-pinene, β-pinene and limonene, reinforcing their roles in host recognition and reproductive functions. This study elaborates on our understanding of the putative roles of different OBPs and CSPs in B. tabaci Asia II-1, hitherto unexplored. The dynamics of the expression of OBPs and CSPs and their interactions with odour compounds offer scope for developing innovative methods for controlling this global invasive pest.

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Transcriptome analysis of megalurothrips usitatus (Bagnall) identifies olfactory genes with ligands binding characteristics of MusiOBP1 and MusiCSP1

Cited by 4 publications

References 59 publications

A Study of Adult Olfactory Proteins of Primitive Ghost Moth, Endoclita signifer (Lepidoptera, Hepialidae)

A Study of Adult Olfactory Proteins of Primitive Ghost Moth, Endoclita signifer (Lepidoptera, Hepialidae)

The Chromosome-Level Genome Assembly of Bean Blossom Thrips (Megalurothrips usitatus) Reveals an Expansion of Protein Digestion-Related Genes in Adaption to High-Protein Host Plants

Variations in the expression of odorant binding and chemosensory proteins in the developmental stages of whitefly Bemisia tabaci Asia II-1

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