Transcriptome Analysis of Monocytic Leukemia Cell Differentiation

Piquemal, David; Commes, Thérèse; Manchon, Laurent; Lejeune, Mireille; Ferraz, Conchita; Pugnère, Denis; Demaille, Jacques; Elalouf, Jean‐Marc; Marti, Jacques

doi:10.1006/geno.2002.6836

Cited by 53 publications

(64 citation statements)

References 37 publications

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“…CREG is a secreted glycoprotein and strikingly, addition of CREG-enriched media to NTERA-2 cells promoted the appearance of neuronal differentiation markers even in the absence of an inducer such as RA (Veal et al, 2000). A number of studies, including our own, have reported the induction of CREG mRNA during differentiation of diverse cell types such as pluripotent mouse ES cells (Veal et al, 2000), monocytes (Piquemal et al, 2002), and myeloid cells (Ma et al, 2002). In addition, in a study of variations in gene expression during mouse CNS development, CREG was found in a cluster of genes whose expression is inversely correlated with cyclin D1 expression (Miki et al, 2001).…”

Section: Introductionmentioning

confidence: 75%

The secreted glycoprotein CREG inhibits cell growth dependent on the mannose-6-phosphate/insulin-like growth factor II receptor

Bacco

Gill

2003

Oncogene

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Secreted proteins and their cognate receptors are implicated in a myriad of activities that regulate cell proliferation, differentiation, and development. CREG, a cellular repressor of E1A-stimulated genes, is a secreted glycoprotein that antagonizes cellular transformation by E1A and ras. We have previously shown that CREG expression is induced very early during differentiation of pluripotent cells and, even in the absence of other inducers, CREG promotes neuronal differentiation of human teratocarcinoma NTERA-2 cells. Here we show that ectopic expression of CREG in NTERA-2 cells results in a delay of the G1/S phase transition of the cell cycle and growth inhibition. We show that CREG binds directly to the mannose-6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) dependent on CREG glycosylation. The M6P/IGF2R is a tumor suppressor that functions to control cell growth through interactions with multiple ligands. By analysing CREG activity in cells lacking M6P/IGF2R expression, we show that this receptor is required for CREG-induced growth inhibition. These studies reveal that CREG inhibits cell growth dependent on the M6P/IGF2R and suggest that interactions between CREG and a well-characterized tumor suppressor may contribute to regulation of proliferation and differentiation in multiple lineages.

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Section: Introductionmentioning

confidence: 75%

The secreted glycoprotein CREG inhibits cell growth dependent on the mannose-6-phosphate/insulin-like growth factor II receptor

Bacco

Gill

2003

Oncogene

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“…Bioinformatic comparisons [30] in several genomic databases (Unigen, Tigr) allowed firstly to identify the different activated and inactivated tags (known genes, EST or unknown genes) and secondly to compare their respective frequencies in both D0L and MPL libraries.…”

Section: The Sage Methodsmentioning

confidence: 99%

Serial analysis of gene expression (SAGE) in bovine trypanotolerance: preliminary results

Berthier

Quéré²,

Thévenon

et al. 2003

Genet. Sel. Evol.

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-In Africa, trypanosomosis is a tsetse-transmitted disease which represents the most important constraint to livestock production. Several indigenous West African taurine (Bos taurus) breeds, such as the Longhorn (N'Dama) cattle are well known to control trypanosome infections. This genetic ability named "trypanotolerance" results from various biological mechanisms under multigenic control. The methodologies used so far have not succeeded in identifying the complete pool of genes involved in trypanotolerance. New post genomic biotechnologies such as transcriptome analyses are efficient in characterising the pool of genes involved in the expression of specific biological functions. We used the serial analysis of gene expression (SAGE) technique to construct, from Peripheral Blood Mononuclear Cells of an N'Dama cow, 2 total mRNA transcript libraries, at day 0 of a Trypanosoma congolense experimental infection and at day 10 post-infection, corresponding to the peak of parasitaemia. Bioinformatic comparisons in the bovine genomic databases allowed the identification of 187 up-and downregulated genes, EST and unknown functional genes. Identification of the genes involved in trypanotolerance will allow to set up specific microarray sets for further metabolic and pharmacological studies and to design field marker-assisted selection by introgression programmes. SAGE / trypanotolerance / N'Dama / Trypanosoma congolense / transcriptomics

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“…For comparison between libraries, tags with a sum of occurrences below 10 in the two compared sets or with a P>0.005 were discarded. P-value determination was performed as previously described (Piquemal et al, 2002). Tags were mapped using the short sequence mapping tools SeqMap (Jiang and Wong, 2008) and MPscan (Rivals et al, 2009) against the Smed454_90edb transcriptome dataset (Abril et al, 2010).…”

Section: Construction Sequencing and Analysis Of The Dge Librariesmentioning

confidence: 99%

egr-4, a target of EGFR signaling, is required for the formation of the brain primordia and head regeneration in planarians

et al. 2014

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During the regeneration of freshwater planarians, polarity and patterning programs play essential roles in determining whether a head or a tail regenerates at anterior or posterior-facing wounds. This decision is made very soon after amputation. The pivotal role of the Wnt/ β-catenin and Hh signaling pathways in re-establishing anteriorposterior (AP) polarity has been well documented. However, the mechanisms that control the growth and differentiation of the blastema in accordance with its AP identity are less well understood. Previous studies have described a role of Smed-egfr-3, a planarian epidermal growth factor receptor, in blastema growth and differentiation. Here, we identify Smed-egr-4, a zinc-finger transcription factor belonging to the early growth response gene family, as a putative downstream target of Smed-egfr-3. Smed-egr-4 is mainly expressed in the central nervous system and its silencing inhibits anterior regeneration without affecting the regeneration of posterior regions. Single and combinatorial RNA interference to target different elements of the Wnt/β-catenin pathway, together with expression analysis of brain-and anterior-specific markers, revealed that Smed-egr-4: (1) is expressed in two phasesan early Smed-egfr-3-independent phase and a late Smed-egfr-3-dependent phase; (2) is necessary for the differentiation of the brain primordia in the early stages of regeneration; and (3) that it appears to antagonize the activity of the Wnt/β-catenin pathway to allow head regeneration. These results suggest that a conserved EGFR/egr pathway plays an important role in cell differentiation during planarian regeneration and indicate an association between early brain differentiation and the proper progression of head regeneration.

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Transcriptome Analysis of Monocytic Leukemia Cell Differentiation

Cited by 53 publications

References 37 publications

The secreted glycoprotein CREG inhibits cell growth dependent on the mannose-6-phosphate/insulin-like growth factor II receptor

The secreted glycoprotein CREG inhibits cell growth dependent on the mannose-6-phosphate/insulin-like growth factor II receptor

Serial analysis of gene expression (SAGE) in bovine trypanotolerance: preliminary results

egr-4, a target of EGFR signaling, is required for the formation of the brain primordia and head regeneration in planarians

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