Transcriptome Analysis of Morus alba L. Flower Reveals Important Genes of Floral Sex Differentiation

Abstract

Mulberry (Morus alba L.) is a perennial woody plant with significant economic benefits and ecological value. The floral character of mulberry has an important impact on the yield and quality to its fruits and leaves. However, little is known about the molecular mechanism of mulberry floral differentiation still now. The transcriptome data were obtained via Illumina HiSeq high-throughput sequencing from male and female inflorescences of the monoecious mulberry. A total of 26.21 Gb clean data were obtained, and as many as 100,177 unigenes with an average length of 821.66 bp were successfully assembled. In comparative-omics analysis, 1717 differentially expressed genes (DEGs) were identified between male and female flowers and only a quarter of the DEGs were highly expressed in female flowers. The KEGG pathway enrichment analysis revealed that DEGs were involved in glucose and lipid metabolism, hormone signal transduction, and the regulation of related transcription factors. In addition, many DEGs related to flower development and plant sex differentiation have also been detected, such as PMADS1/2, AGAMOUS, FLOWERING LOCUS T (FT), APETALA 2 (AP2), TASSELSEED2 (TS2), and ARABIDOPSIS RESPONSE REGULATOR 17 (ARR17). Finally, the expression patterns of selected 20 DEGs were validated by q-PCR and the results showed that the transcriptome data were highly reliable. This study shows that the differentiation of male and female flowers of mulberry is affected and regulated by multiple factors, with transcription factors and hormone signals playing a key role. Briefly, the current data provide comprehensive insights into the mulberry tree’s floral differentiation as well as a bioinformatics framework for the development of molecular breeding of mulberry.

Genomic Resequencing Unravels the Genetic Basis of Domestication, Expansion, and Trait Improvement in Morus Atropurpurea

“…[ 62 ] This indicates that hormones and external environmental stimulation influence plant sex differentiation by affecting the expression levels of SD genes in mulberry. [ 62 , 63 , 64 ] Ethylene and auxin promote female flower formation, whereas GAs promote male flower formation. [ 65 , 66 ] In this study, the GDSL esterase/lipase gene ( EVM0018167.1 ) was highly expressed (FPKM = 190) in male mulberry, and may be a putative key gene that regulates gas production and is responsible for male flower differentiation.…”