Transcriptome analysis of ovine granulosa cells reveals differences between small antral follicles collected during the follicular and luteal phases

Talebi, Reza; Ahmadi, Ahmad; Afraz, Fazlollah; Sarry, Julien; Plisson-Petit, Florence; Genêt, Carine; Fabre, Stéphane

doi:10.1016/j.theriogenology.2017.11.027

Cited by 17 publications

(14 citation statements)

References 65 publications

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“…So the equivalent of 1.25 oocyte was reverse-transcribed using SuperScript II reverse transcriptase (Invitrogen) and anchored oligo(dT)22 primer (1 µl at 10 µM). Primer design using Beacon designer 8.20 (Premier Biosoft), SYBR green real-time PCR cycling conditions using a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific) and amplification efficiency calculation [E = e (−1/slope) ] were as previously described in Talebi et al (2018a). Primer sequences, amplicon length, and amplification efficiency are listed in Supplementary Table S2.…”

Section: Rna Extraction Reverse Transcription and Quantitative Pcrmentioning

confidence: 99%

Genome-Wide Identification of a Regulatory Mutation in BMP15 Controlling Prolificacy in Sheep

et al. 2020

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The search for the genetic determinism of prolificacy variability in sheep has evidenced several major mutations in genes playing a crucial role in the control of ovulation rate. In the Noire du Velay (NV) sheep population, a recent genetic study has evidenced the segregation of such a mutation named FecL L . However, based on litter size (LS) records of FecL L non-carrier ewes, the segregation of a second prolificacy major mutation was suspected in this population. In order to identify this mutation, we have combined a case/control genome-wide association study with ovine 50k SNP chip genotyping, whole genome sequencing, and functional analyses. A new single nucleotide polymorphism (OARX:50977717T > A, NC_019484) located on the X chromosome upstream of the BMP15 gene was evidenced to be highly associated with the prolificacy variability (P = 1.93E-11). The variant allele was called FecX N and shown to segregate also in the Blanche du Massif Central (BMC) sheep population. In both NV and BMC, the FecX N allele frequency was estimated close to 0.10, and its effect on LS was estimated at +0.20 lamb per lambing at the heterozygous state. Homozygous FecX N carrier ewes were fertile with increased prolificacy in contrast to numerous mutations affecting BMP15. At the molecular level, FecX N was shown to decrease BMP15 promoter activity and supposed to impact BMP15 expression in the oocyte. This regulatory action was proposed as the causal mechanism for the FecX N mutation to control ovulation rate and prolificacy in sheep.

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Section: Rna Extraction Reverse Transcription and Quantitative Pcrmentioning

confidence: 99%

Genome-Wide Identification of a Regulatory Mutation in BMP15 Controlling Prolificacy in Sheep

et al. 2020

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“…Interestingly, such hubs all were belonged to module 12 whose been enriched in positive regulation of DNA, cell cycle and progesterone-mediated oocyte (Table 2 ). Therefore, the hubs including CDK1 , INSRR and TOP2A , represented the proliferation of ovine granulosa cells from small antral follicles during the luteal phase [ 20 ], whose probably are crucial in cyclic recruitment of small antral follicles. This is closer to the second theory in recruitment of antral follicles during menstrual cycle by Baerwald et al [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…The main dataset of the present study was belonged to 663 DEGs in ovine granulosa cells between small antral follicles (1–3 mm in diameter) collected during the follicular and luteal phases [ 20 ]. These DEGs afterwards were annotated using a standard Ensembl gene annotation system.…”

Section: Methodsmentioning

confidence: 99%

“…In previous study, we surprisingly have shown differences in transcriptome profiles of ovine (sheep) granulosa cells between small antral follicles (1–3 mm) collected during the follicular and luteal phases [ 20 ]. Therefore, the specific purpose of the present study was to survey the differentially expressed genes (DEGs) from the previous study using the analysis of PPI networks in order to identification of candidate genes that probably those are impressive in cyclic recruitment of ovarian follicles.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of protein-protein interaction network based on transcriptome profiling of ovine granulosa cells identifies candidate genes in cyclic recruitment of ovarian follicles

2018

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After pubertal, cohort of small antral follicles enters to gonadotrophin-sensitive development, called recruited follicles. This study was aimed to identify candidate genes in follicular cyclic recruitment via analysis of protein-protein interaction (PPI) network. Differentially expressed genes (DEGs) in ovine granulosa cells of small antral follicles between follicular and luteal phases were accumulated among gene/protein symbols of the Ensembl annotation. Following directed graphs, PTPN6 and FYN have the highest indegree and outdegree, respectively. Since, these hubs being up-regulated in ovine granulosa cells of small antral follicles during the follicular phase, it represents an accumulation of blood immune cells in follicular phase in comparison with luteal phase. By contrast, the up-regulated hubs in the luteal phase including CDK1, INSRR and TOP2A which stimulated DNA replication and proliferation of granulosa cells, they known as candidate genes of the cyclic recruitment.Electronic supplementary materialThe online version of this article (10.1186/s40781-018-0171-y) contains supplementary material, which is available to authorized users.

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“…In addition, Liu et al proposed that ovulation involved the expression and function of molecules that exert potent roles in innate immune responses, and granulosa cells appeared to play immuno-protective-like functions for the ovulated oocytes [26]. Another report found that the possible initiation of early follicular atresia in small antral follicles during the follicular phase had an interaction with the presence of immune cells [27]. Our results suggest that up-regulation of CD83 in lamb GCs may have an adverse effect on the immune system and development of oocytes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs

Tian

Ren

Liu

et al. 2021

Preprint

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Background The oocyte development ability of prepubertal animals is significantly lower than that of adult animals. Granulosa cells (GCs) have an important function on regulation of follicular and oocyte development. Therefore, analysis of GCs characteristics can be used to explore the developmental mechanism of follicles and oocytes. Results In order to understand the possible reasons for the differences in follicles and oocytes development between lambs and adult sheep, we utilized high-throughput sequencing technique to analyze the transcriptome of GCs from follicle-stimulating hormone (FSH) superstimulated adult ewes and prepubertal lambs. Adult ewes were stimulated with FSH for 3 days (group A) and lambs were FSH-stimulated for 2 days (group B) or 3 days (group C). Transcriptome analysis of GCs showed that there were 405 and 159 differentially expressed genes from A vs. B and A vs. C, respectively. The results indicated that prolonging the FSH-stimulation of lambs made the GC state of lambs more similar to the adult sheep, but there were still a large number of differentially expressed genes between adult sheep and lambs. Further analysis showed that many differently expressed genes were implicated in cell proliferation and apoptosis, oocyte development and follicular ovulation. Cellular examination demonstrated that fatty acid binding protein 4 (FABP4), which was highly expressed in lamb GCs, had a potential of promoting cell apoptosis. Cytoplasmic phospholipase A2 (PLA2G4A), which was expressed lowly in lamb GCs, may be responsible for reduced synthesis of prostaglandins in cells and impaired follicle/oocyte development. In contrast, glutathione S-transferase β-1 (GSTT2B) and forkhead boxO6 (FOXO6) had no apparent effect on the proliferation and apoptosis of GCs.

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Transcriptome analysis of ovine granulosa cells reveals differences between small antral follicles collected during the follicular and luteal phases

Cited by 17 publications

References 65 publications

Genome-Wide Identification of a Regulatory Mutation in BMP15 Controlling Prolificacy in Sheep

Genome-Wide Identification of a Regulatory Mutation in BMP15 Controlling Prolificacy in Sheep

Analysis of protein-protein interaction network based on transcriptome profiling of ovine granulosa cells identifies candidate genes in cyclic recruitment of ovarian follicles

Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs

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