Transcriptome analysis of peach fruit under 1-MCP treatment provides insights into regulation network in melting peach softening

Qian, Jianpu; Zhao, Yingjie; Shi, Yanna; Chen, Kunsong

doi:10.1093/fqsafe/fyac048

Cited by 7 publications

(2 citation statements)

References 60 publications

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“…Fruit at the B3 stage was used for ethylene measurement following a previously described method with minor revisions ( Qian et al 2022 ). Fruit was harvested and left undisturbed on a bench for 4 h and then sealed in a 300-ml buckle box at room temperature for 2 h before being tested with a 1-ml syringe absorbing headspace air for ethylene.…”

Section: Methodsmentioning

confidence: 99%

Expansin SlExp1 and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato

Su,

Lin,

Wang

et al. 2023

The Plant Cell

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Fruit softening, an irreversible process that occurs during fruit ripening, can lead to losses and waste during postharvest transportation and storage. Cell wall disassembly is the main factor leading to loss of fruit firmness, and several ripening-associated cell wall genes have been targeted for genetic modification, particularly pectin modifiers. However, individual knockdown of most cell wall–related genes has had minimal influence on cell wall integrity and fruit firmness, with the notable exception of pectate lyase. Compared to pectin disassembly, studies of the cell wall matrix, the xyloglucan–cellulose framework, and underlying mechanisms during fruit softening are limited. Here, a tomato (Solanum lycopersicum) fruit ripening–associated α-expansin (SlExpansin1/SlExp1) and an endoglucanase (SlCellulase2/SlCel2), which function in the cell wall matrix, were knocked out individually and together using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9-mediated genome editing. Simultaneous knockout of SlExp1 and SlCel2 enhanced fruit firmness, reduced depolymerization of homogalacturonan-type pectin and xyloglucan, and increased cell adhesion. In contrast, single knockouts of either SlExp1 or SlCel2 did not substantially change fruit firmness, while simultaneous overexpression of SlExp1 and SlCel2 promoted early fruit softening. Collectively, our results demonstrate that SlExp1 and SlCel2 synergistically regulate cell wall disassembly and fruit softening in tomato.

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Section: Methodsmentioning

confidence: 99%

Expansin SlExp1 and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato

Su,

Lin,

Wang

et al. 2023

The Plant Cell

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“…The significant RDR-associated SNP on Ra_g5252 was positioned only 1,355 bp from the closest fruit firmness SNP. Prupe.8G098000, a homolog Ra_g5252 found in peach, is a β-glucosidase that is upregulated by treatment with 1-MCP ( Qian et al., 2022 ), and the authors have speculated that it may be important in strengthening cell walls. Although the role of β-glucosidases in fruit texture has been well documented in several species ( Gerardi et al., 2001 ; Konozy et al., 2012 ; Ortiz Araque et al., 2019 ), few studies have demonstrated genetic variation β-glucosidase and its potential connection to phenotypic variation for fruit texture.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association identifies key loci controlling blackberry postharvest quality

et al. 2023

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IntroductionBlackberry (Rubus subgenus Rubus) is a soft-fruited specialty crop that often suffers economic losses due to degradation in the shipping process. During transportation, fresh-market blackberries commonly leak, decay, deform, or become discolored through a disorder known as red drupelet reversion (RDR). Over the past 50 years, breeding programs have achieved better fruit firmness and postharvest quality through traditional selection methods, but the underlying genetic variation is poorly understood.MethodsWe conducted a genome-wide association of fruit firmness and RDR measured in 300 tetraploid fresh-market blackberry genotypes from 2019-2021 with 65,995 SNPs concentrated in genic regions of the R. argutus reference genome.ResultsFruit firmness and RDR had entry-mean broad sense heritabilities of 68% and 34%, respectively. Three variants on homologs of polygalacturonase (PG), pectin methylesterase (PME), and glucan endo-1,3-β-glucosidase explained 27% of variance in fruit firmness and were located on chromosomes Ra06, Ra01, and Ra02, respectively. Another PG homolog variant on chromosome Ra02 explained 8% of variance in RDR, but it was in strong linkage disequilibrium with 212 other RDR-associated SNPs across a 23 Mb region. A large cluster of six PME and PME inhibitor homologs was located near the fruit firmness quantitative trait locus (QTL) identified on Ra01. RDR and fruit firmness shared a significant negative correlation (r = -0.28) and overlapping QTL regions on Ra02 in this study.DiscussionOur work demonstrates the complex nature of postharvest quality traits in blackberry, which are likely controlled by many small-effect QTLs. This study is the first large-scale effort to map the genetic control of quantitative traits in blackberry and provides a strong framework for future GWAS. Phenotypic and genotypic datasets may be used to train genomic selection models that target the improvement of postharvest quality.

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Comparative analysis of fruit firmness and genes associated with cell wall metabolisms in three cultivated strawberries during ripening and postharvest

Ren

Jia

et al. 2023

Food Quality and Safety

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Cultivated strawberry (Fragaria × ananassa), a world-famous fruit, is subjected to rapid softening during ripening, resulting in a shorter shelf life and severe economic losses during storage and transportation. However, there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries. Here, we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement, transcriptome analysis, quantitative real-time PCR, and correlation analysis, and revealed that FaEXP7, FaPG2, FaPLA, and Faβ-Gal4, as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life, and the extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation. Additionally, both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest, suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest. These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.

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Transcriptome analysis of peach fruit under 1-MCP treatment provides insights into regulation network in melting peach softening

Cited by 7 publications

References 60 publications

Expansin SlExp1 and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato

Expansin SlExp1 and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato

Genome-wide association identifies key loci controlling blackberry postharvest quality

Comparative analysis of fruit firmness and genes associated with cell wall metabolisms in three cultivated strawberries during ripening and postharvest

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