Transcriptome Analysis of Purple Pericarps in Common Wheat (Triticum aestivum L.)

Liu, Di; Li, Shiming; Chen, Wenjie; Zhang, Bo; Liu, Dengcai; Liu, Baolong; Zhang, Huaigang

doi:10.1371/journal.pone.0155428

Cited by 28 publications

(35 citation statements)

References 30 publications

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“…The transient expression of TaMYB3 alone did not induce anthocyanin biosynthesis in the white pericarp of Opata, whereas the transient expression of ZmR did. As mentioned in the introduction, the bHLH transcriptor TaMYC1 was thought to be encoded by the candidate gene for Pp3 (Shoeva et al 2014;Liu et al 2016). The transient expression of ZmR compensated for TaMYC1 in the white pericarp of Opata.…”

Section: Discussionmentioning

confidence: 99%

“…Among the genes associated with anthocyanin biosynthesis in Triticum aestivum, the red grain (R) and red coleoptile (Rc) genes have been shown to encode R2R3-MYB transcription factors that regulate proanthocyanidin and anthocyanin biosynthesis in grains and coleoptiles, respectively (Himi et al 2011;Himi and Taketa 2015;Wang et al 2016). The candidate gene for the purple pericarp trait in common wheat, Pp3, is the basic helix-loop-helix (bHLH) transcriptor TaMYC1 (Shoeva et al 2014;Liu et al 2016), and the Pp1 genes are thought to be orthologues of maize C1 and rice OsC1, which encode MYB-like transcription factors responsible for the activation of the structural genes encoding various enzymes that participate in anthocyanin synthesis (Saitoh et al 2004;Khlestkina 2013). However, the function of the MYB transcriptor in the purple grain trait of common wheat has not been analyzed.…”

Section: Introductionmentioning

confidence: 99%

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TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Zong

Liu

et al. 2017

Cereal Research Communications

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Purple pericarp is an interesting and useful trait in Triticum aestivum, but the molecular mechanism behind this phenotype remains unclear. The allelic variation in the MYB transcriptors is associated with the phenotype of pigmented organs in many plants. In this study, a MYB transcription factor gene, TaMYB3, was isolated using homology-based cloning and a differentially expressed gene mining approach, to verify the function of the MYB transcriptor in the purple pericarp. The coding sequence of TaMYB3 in cultivar Gy115 was the same as that in cultivar Opata. TaMYB3 was localized to FL0.62-0.95 on chromosome 4BL. The TaMYB3 protein contains DNA-binding and transcription-activation domains, and clustered on a phylogenetic tree with the MYB proteins that regulates anthocyanin and proanthocyanin biosynthesis. TaMYB3 localized in the nuclei of Arabidopsis thaliana and wheat protoplasts after it was transiently expressed with PEG transformation. TaMYB3 induced anthocyanin synthesis in the pericarp cells of Opata in the dark in collaboration with the basic helix-loop-helix protein ZmR, which is also the function of ZmC1. However, TaMYB3 alone did not induce anthocyanin biosynthesis in the pericarp cells of the white grain wheat cultivar Opata in the light after bombardment, whereas the single protein ZmR did. Light increased the expression of TaMYB3 in the pericarp of Gy115 and Opata, but only induced anthocyanin biosynthesis in the grains of Gy115. Our results extend our understanding of the molecular mechanism of the purple pericarp trait in T. aestivum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Zong

Liu

et al. 2017

Cereal Research Communications

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“…In particular, sterically stabilized 1,2‐dioxetanes, such as Schaap's dioxetane enable reaction‐based triggering of chemiluminescence emission using a chemically initiated electron exchange luminescence (CIEEL) mechanism . This strategy has been used to image numerous analytes in vitro, in living cells, and in whole animals . Recent advances in molecular design have yielded structures with high emission under aqueous conditions without the need for polymeric “Enhancer” solutions .…”

Section: Methodsmentioning

confidence: 99%

A Chemiluminescent Probe for HNO Quantification and Real‐Time Monitoring in Living Cells

et al. 2018

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Azanone (HNO) is a reactive nitrogen species with pronounced biological activity and high therapeutic potential for cardiovascular dysfunction. A critical barrier to understanding the biology of HNO and furthering clinical development is the quantification and real‐time monitoring of its delivery in living systems. Herein, we describe the design and synthesis of the first chemiluminescent probe for HNO, HNOCL‐1, which can detect HNO generated from concentrations of Angeli's salt as low as 138 nm with high selectivity based on the reaction with a phosphine group to form a self‐cleavable azaylide intermediate. We have capitalized on this high sensitivity to develop a generalizable kinetics‐based approach, which provides real‐time quantitative measurements of HNO concentration at the picomolar level. HNOCL‐1 can monitor dynamics of HNO delivery in living cells and tissues, demonstrating the versatility of this method for tracking HNO in living systems.

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“…In 2016, Liu et al [168] compared the transcriptomes of purple and white pericarps in common wheat and reported significant differential expression of a total of 23,642 unigenes (9945 up-regulated and 13,697 downregulated). The analysis predicted three unigenes of MYB gene on the long arm of the chromosome 7B and three unigenes of MYC on the long arm of the chromosome 2A as candidate genes for the purple grain trait.…”

Section: Genetic Basis Of Purple Coloured Wheat Grainsmentioning

confidence: 99%

QTLs identified for Biofortification Traits in Wheat: A Review

Devi¹,

Kaushik²,

Saini³

2019

Preprint

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Wheat is the essential constituent of cereal-based diets and one of the most significant sources of calories. However, there is an inherently low bioavailability of proteins, mineral, and vitamins in modern wheat grains. Biofortification has earned recognition as an outstanding approach, at the same time as a cure for world hunger. The developments in the identifications of quantitative trait loci (QTL) analysis and understanding of the physiological and molecular basis of QTLs controlling the biofortification traits in wheat has revealed new horizons for the improvement of modern wheat varieties. Within this review, we have compiled the information from the studies carried out in wheat using QTL mapping methodologies that is among the best methods for biofortification traits. We hope this review will serve as an essential reference for the QTLs identified for the several important biofortification traits in wheat.that could contribute to underexploited wild relatives [25,26]. The different steps involved in the development of biofortification wheat with the aid of marker-assisted breeding are presented in Figure1. Figure 1.Representation of different strategies for biofortification of the wheat, especially explaining different steps of marker-assisted breeding.Conventional breeding methods are easy to operate with qualitative traits. These traits depend on a single gene whereas traits like yield are quantitative and therefore are impacted by several genes [27,28,29]. There are several techniques for mapping quantitative trait loci (QTL) in an experimental cross [30]. The molecular basis of QTLs is challenging to dissect, even for model plants like Arabidopsis and rice, because of the problems in precisely narrowing intervals to single genes [31]. Experimental design, type of plant population analysed and the level of polymorphisms between parental genomes also affect the predictions of QTLs. Statistical methods to determine quantitative trait loci (QTL) require numerous molecular markers with high-resolution genetic maps [32,33]. This approach is also related to genomics methods which are geared toward the dissection of complex phenotypes [34].Genomic resources of wheat have provided important support for functional genomics and conservation biology (by conserving the important landraces) [35,36]. The wheat genome is complex to interpret simply because of broadly dispersed repetitive sequences, heterozygosity, and polyploidy [37,38]. Nevertheless, the developments in sequencing methodologies, decreased sequencing price, together with the advancements in the computational resources have permitted the spread of these resources [39,40]. Besides, the comparative genomics among plant species is demonstrating to be an efficient method for the identification of novel genes regarding the biofortification of modern wheat [41]. Therefore, in this review, we have compiled the QTLs Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Transcriptome Analysis of Purple Pericarps in Common Wheat (Triticum aestivum L.)

Cited by 28 publications

References 30 publications

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

A Chemiluminescent Probe for HNO Quantification and Real‐Time Monitoring in Living Cells

QTLs identified for Biofortification Traits in Wheat: A Review

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