Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

Habu, Toshiyuki; Tao, Ryutaro

doi:10.2503/jjshs1.ch-086

Cited by 16 publications

(15 citation statements)

References 56 publications

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“…Furthermore, we used two other transcriptomes from P . mume cultivar Nanko for unpollinated pistils and pollen (DRR013977, and DRR002283, respectively; [ 63 ]). We applied the same methodology as in the Malus expression analyses, using as query sequences for the S -locus genes S-RNase scaffold 241.33 and SFB scaffold 241.2 for cultivar landrace, and S1-RNase (BAF91149.1), S7-RNase (BAF91155.1), SFB1 (BAD08320.1) and SFB7 (BAD08321.1) for cultivar Nanko.…”

Section: Methodsmentioning

confidence: 99%

Convergent Evolution at the Gametophytic Self-Incompatibility System in Malus and Prunus

et al. 2015

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S-RNase-based gametophytic self-incompatibility (GSI) has evolved once before the split of the Asteridae and Rosidae. This conclusion is based on the phylogenetic history of the S-RNase that determines pistil specificity. In Rosaceae, molecular characterizations of Prunus species, and species from the tribe Pyreae (i.e., Malus, Pyrus, Sorbus) revealed different numbers of genes determining S-pollen specificity. In Prunus only one pistil and pollen gene determine GSI, while in Pyreae there is one pistil but multiple pollen genes, implying different specificity recognition mechanisms. It is thus conceivable that within Rosaceae the genes involved in GSI in the two lineages are not orthologous but possibly paralogous. To address this hypothesis we characterised the S-RNase lineage and S-pollen lineage genes present in the genomes of five Rosaceae species from three genera: M. × domestica (apple, self-incompatible (SI); tribe Pyreae), P. persica (peach, self-compatible (SC); Amygdaleae), P. mume (mei, SI; Amygdaleae), Fragaria vesca (strawberry, SC; Potentilleae), and F. nipponica (mori-ichigo, SI; Potentilleae). Phylogenetic analyses revealed that the Malus and Prunus S-RNase and S-pollen genes belong to distinct gene lineages, and that only Prunus S-RNase and SFB-lineage genes are present in Fragaria. Thus, S-RNase based GSI system of Malus evolved independently from the ancestral system of Rosaceae. Using expression patterns based on RNA-seq data, the ancestral S-RNase lineage gene is inferred to be expressed in pistils only, while the ancestral S-pollen lineage gene is inferred to be expressed in tissues other than pollen.

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Section: Methodsmentioning

confidence: 99%

Convergent Evolution at the Gametophytic Self-Incompatibility System in Malus and Prunus

et al. 2015

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“…The detection of a disrupted SLFL1 gene in a functional self-incompatible S haplotype indicates that SLFL1 is not the only factor necessary for cross compatibility. The most likely GI candidate is SLFL3 because of the SLFL genes, only SLFL3 exhibits upregulated expression during compatible pollination (Habu and Tao, 2013). The Prunus homolog of SBP1 is another candidate for GI.…”

Section: Working Model For Self/nonself Discrimination In Prunus Speciesmentioning

confidence: 99%

“…Cytoskeletal changes may be involved in the Prunus SI reaction because the artificially reduced S-RNases (in a reducing intracellular environment) interact with actin in vitro (Matsumoto and Tao, 2012b). Habu and Tao (2013) recently identified thousands of genes differentially regulated between self-and cross-pollinated pistils using next-generation sequencing technologies. Interestingly, no homologs of the solanaceous pistil factors, such as HT-B or120K, were detected, indicating there is a distinct Prunus SI reaction.…”

Section: Self-incompatibility Events That Arrest Self-pollen Tube Gromentioning

confidence: 99%

“…Interestingly, the modifier loci of sweet cherry and apricot cultivars were located at the distal end of linkage group 3 (M locus), which is the syntenic region of the Maleae S locus Wünsch, 2011, 2014;Zuriaga et al, 2012Zuriaga et al, , 2013. However, none of genes reported to be involved in SI are located in this region (Habu and Tao, 2013;Zuriaga et al, 2012). Identifying the pollen modifier gene will provide new insights into the Prunus-specific SI mechanism.…”

Section: Self-incompatibility Events That Arrest Self-pollen Tube Gromentioning

confidence: 99%

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Distinct Self-recognition in the Prunus S-RNase-based Gametophytic Self-incompatibility System

Matsumoto

Tao

2016

The Hortic J

Self Cite

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Prunus fruit tree species exhibit S-ribonuclease (S-RNase)-based gametophytic self-incompatibility (GSI). This system is also present in the families Plantaginaceae and Solanaceae and the tribe Maleae of the family Rosaceae. In S-RNase-based GSI, self/nonself-recognition between the pistil and pollen is controlled by the pistil S determinant S-ribonuclease gene (S-RNase) and the pollen S determinant F-box gene(s). Accumulated evidence indicates the Prunus pollen S locus contains a single F-box gene, while that of other plants consists of multiple F-box genes. The pollen S F-box genes are called S haplotype-specific F-box (SFB), S-locus F-box brothers (SFBB), and S-locus F-box (SLF) in Prunus, Maleae, and Solanaceae species, respectively. The consequences of pollen S gene mutations and heterodiallelic pollen production differ between Prunus species and other plants, suggesting there are different pollen S functions during self/nonself-recognition. The GSI systems of Prunus and other plants are believed to include the ubiquitin proteasome system for protein degradation. However, Prunus SFB is assumed to facilitate the S-RNase cytotoxic effects during selfrecognition, while SLFs and SFBBs are thought to function collaboratively during nonself-recognition to avoid S-RNase cytotoxicity. This review summarizes the distinct features of the S-RNase-based GSI mechanism in Prunus species, with special references to the recent advances in our understanding of S-RNase-based GSI.

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“…Special attention must be given to the selection of varieties and plant spacing to avoid fruitlessness and low fruitfulness, which will otherwise impair the ornamental and application values of Rosa rugosa. It is important to overcome the self-incompatibility of Rosa rugosa and breed new varieties of ornamental fruit rugosa with self-compatibility [4] [5]. But so far the mechanism of self-incompatibility of Rosa rugosa has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Bioinformatics Analysis of Rosa rugose S Locus F-Box Gene (RrSLF)

Wei¹,

Li²,

Xing³

et al. 2017

AJPS

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In order to reveal the phenomenon of R. rugosa pollination incompatibility, the full-length cDNA sequence of S Locus F-box Gene was cloned for the first time from the pollen of Rosa rugosa "Zilong wochi" with RT-PCR and RACE methods and named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino acids. The derived protein has a molecular weight of 43.7 kD, a calculated pI of 6.24, an F-box conserved domain at position 343 -741, and belongs to F-box family. The derived protein is a Hydrophobicity protein secreted into the cytoplasm. There is no transmembrane domain and no signal peptide cleavage site, twenty-one Ser phosphorylation sites, seven Thr phosphorylation sites, seven Tyr phosphorylation sites, two N-glycosylation sites, and no O-glycosylation sites. There are 22.25% α-helixes, 31.37% random coil, 32.17% extended peptide chain, and 14.21% β-corner structure. This protein and the SFB/SLF protein from Rosaceae Prunus fruit, including Prunus speciosa, share a sequence homology of 59% -61%; all of the proteins contain an F-box conserved domain, two hypervariable regions HVa, HVb, and two variable regions V1, V2. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results were meaningful to reveal the molecular mechanism of Rosa rugosa pollination incompatibility and improve the theory and techniques of breeding ornamental Rosa rugosa.

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Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

Cited by 16 publications

References 56 publications

Convergent Evolution at the Gametophytic Self-Incompatibility System in Malus and Prunus

Convergent Evolution at the Gametophytic Self-Incompatibility System in Malus and Prunus

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Cloning and Bioinformatics Analysis of <i>Rosa rugose S</i> Locus F-Box Gene (<i>RrSLF</i>)

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Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

Cited by 16 publications

References 56 publications

Convergent Evolution at the Gametophytic Self-Incompatibility System in Malus and Prunus

Convergent Evolution at the Gametophytic Self-Incompatibility System in Malus and Prunus

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Cloning and Bioinformatics Analysis of &lt;i&gt;Rosa rugose S&lt;/i&gt; Locus F-Box Gene (&lt;i&gt;RrSLF&lt;/i&gt;)

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Cloning and Bioinformatics Analysis of <i>Rosa rugose S</i> Locus F-Box Gene (<i>RrSLF</i>)