Transcriptome Analysis of Sophora davidii Leaves in Response to Low-Phosphorus Stress

Xin, Zhao; Huang, Lijuan; Zhao, Lili; Wang, Pu-Chang; Sun, Xiao-Fu

doi:10.1007/s00344-021-10382-7

Cited by 8 publications

(3 citation statements)

References 65 publications

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“…Sophora davidii seeds were obtained from the Grassland Science Laboratory of the College of Animal Science of Guizhou University of China and planted in plastic pots (43 cm in length × 21 cm in width × 14 cm in height; approximately 30 plants per pot) at Guizhou University (26°27'N, 106°39'E), Huaxi district, Guiyang, Guizhou Province, China. Sophora davidii is widely grown in Southwest China ( Zhao et al, 2021a ). Nutrient-rich soil that included clay was mixed at a 1:1 ratio.…”

Section: Methodsmentioning

confidence: 99%

“…Skeels, a leguminous shrub of the genus Sophora , is an important dominant species. Because of its advantages, such as drought tolerance, tolerance to infertility, strong adaptability and rich nutritional and medicinal value, S. davidii is cultivated as an important plant resource for karst ecological environment management, forage farm establishment and soil improvement ( Yang et al, 2019 ; Gao et al, 2020 ; Ma et al, 2020 ; Zhao et al, 2021a ). At present, research on S. davidii drought stress has focused mainly on the morphological structure and physiological and biochemical responses of this species ( Zhao et al, 2017 ; Zhang et al, 2020 ), but the underlying molecular mechanisms are still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

et al. 2022

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Soil aridification and desertification are particularly prominent in China’s karst areas, severely limiting crop yields and vegetation restoration. Therefore, it is very important to identify naturally drought-tolerant plant species. Sophora davidii (Franch.) Skeels is resistant to drought and soil infertility, is deeply rooted and is an excellent plant material for soil and water conservation. We studied the transcriptomic and metabolomic changes in S. davidii in response to drought stress (CK, control; LD, mild drought stress; MD, moderate drought stress; and SD, severe drought stress). Sophora davidii grew normally under LD and MD stress but was inhibited under SD stress; the malondialdehyde (MDA), hydrogen peroxide (H2O2), soluble sugar, proline, chlorophyll a, chlorophyll b and carotenoid contents and ascorbate peroxidase (APX) activity significantly increased, while the superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and soluble protein content significantly decreased. In the LD/CK, MD/CK and SD/CK comparison groups, there were 318, 734 and 1779 DEGs, respectively, and 100, 168 and 281 differentially accumulated metabolites, respectively. Combined analysis of the transcriptomic and metabolomic data revealed the metabolic regulation of S. davidii in response to drought stress. First, key candidate genes such as PRR7, PRR5, GI, ELF3, PsbQ, PsaK, INV, AMY, E2.4.1.13, E3.2.1.2, NCED, PP2C, PYL, ABF, WRKY33, P5CS, PRODH, AOC3, HPD, GPX, GST, CAT and SOD1 may govern the drought resistance of S. davidii. Second, three metabolites (oxidised glutathione, abscisic acid and phenylalanine) were found to be related to drought tolerance. Third, several key candidate genes and metabolites involved in 10 metabolic pathways were identified, indicating that these metabolic pathways play an important role in the response to drought in S. davidii and possibly other plant species.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

et al. 2022

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metabolism [1,2]. The availability of active P in soil and the efficiency of its uptake by plants significantly influence crop yield and quality.

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confidence: 99%

Transcriptome analysis of Pennisetum americanum × Pennisetum purpureum and Pennisetum americanum leaves in response to high-phosphorus stress

Zhao,

Huang

et al. 2024

BMC Plant Biol

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Excessive phosphorus (P) levels can disrupt nutrient balance in plants, adversely affecting growth. The molecular responses of Pennisetum species to high phosphorus stress remain poorly understood. This study examined two Pennisetum species, Pennisetum americanum × Pennisetum purpureum and Pennisetum americanum, under varying P concentrations (200, 600 and 1000 µmol·L− 1 KH2PO4) to elucidate transcriptomic alterations under high-P conditions. Our findings revealed that P. americanum exhibited stronger adaption to high-P stress compared to P. americanum× P. purpureum. Both species showed an increase in plant height and leaf P content under elevated P levels, with P. americanum demonstrating greater height and higher P content than P. americanum× P. purpureum. Transcriptomic analysis identified significant up- and down-regulation of key genes (e.g. SAUR, GH3, AHP, PIF4, PYL, GST, GPX, GSR, CAT, SOD1, CHS, ANR, P5CS and PsbO) involved in plant hormone signal transduction, glutathione metabolism, peroxisomes, flavonoid biosynthesis, amino acid biosynthesis and photosynthesis pathways. Compared with P. americanum× P. purpureum, P. americanum has more key genes in the KEGG pathway, and some genes have higher expression levels. These results contribute valuable insights into the molecular mechanisms governing high-P stress in Pennisetum species and offer implications for broader plant stress research.

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Effects of Arbuscular Mycorrhizal Fungi on the Growth and Physiological Performance of Sophora davidii Seedling Under Low-Phosphorus Stress

Zhao,

Wang,

Chen

et al. 2024

J Plant Growth Regul

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Transcriptome Analysis of Sophora davidii Leaves in Response to Low-Phosphorus Stress

Cited by 8 publications

References 65 publications

Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

Transcriptome analysis of Pennisetum americanum × Pennisetum purpureum and Pennisetum americanum leaves in response to high-phosphorus stress

Effects of Arbuscular Mycorrhizal Fungi on the Growth and Physiological Performance of Sophora davidii Seedling Under Low-Phosphorus Stress

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