Transcriptome analysis provides insights into the function of the cholinergic system in pearl oyster transplantation immunity

Cao, Yanyan; Jiao, Yu; Zheng, Cong; Zhan, Shuzhi; Gu, Zefeng; Xiong, Xinwei; Wang, Qingheng; Du, Xiaodong

doi:10.1016/j.aquaculture.2021.737205

Cited by 9 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cholinergic system is implicated in innate immunity and inflammation in bivalve mollusks (44)(45)(46)(47). Additionally, it has been described that the cholinergic system regulates the ability of DNA damage repair, and apoptosis in transplanted pearl oyster by affecting Ca 2+ , NF-kB, JAK/STAT, and MAPK signaling pathways (48). In the cholinergic system, acetylcholine is a key neurotransmitter in numerous physiological functions (49).…”

Section: Discussionmentioning

confidence: 99%

Lipidomic insights into the immune response and pearl formation in transplanted pearl oyster Pinctada fucata martensii

Yang²,

Hao³

et al. 2022

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

During pearl culture, the excess immune responses may induce nucleus rejection and death of pearl oysters after transplantation. To better understand the immune response and pearl formation, lipidomic analysis was applied to investigate changes in the serum lipid profile of pearl oyster Pinctada fucata martensii following transplantation. In total, 296 lipid species were identified by absolute quantitation. During wound healing, the content of TG and DG initially increased and then decreased after 3 days of transplantation with no significant differences, while the level of C22:6 decreased significantly on days 1 and 3. In the early stages of transplantation, sphingosine was upregulated, whereas PC and PUFAs were downregulated in transplanted pearl oyster. PI was upregulated during pearl sac development stages. GP and LC-PUFA levels were upregulated during pearl formation stage. In order to identify enriched metabolic pathways, pathway enrichment analysis was conducted. Five metabolic pathways were found significantly enriched, namely glycosylphosphatidylinositol-anchor biosynthesis, glycerophospholipid metabolism, alpha-linolenic acid metabolism, linoleic acid metabolism and arachidonic acid metabolism. Herein, results suggested that the lipids involved in immune response, pearl sac maturation, and pearl formation in the host pearl oyster after transplantation, which might lead to an improvement in the survival rate and pearl quality of transplanted pearl oyster.

show abstract

Section: Discussionmentioning

confidence: 99%

Lipidomic insights into the immune response and pearl formation in transplanted pearl oyster Pinctada fucata martensii

Yang²,

Hao³

et al. 2022

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Transcriptomes analysis revealed many genes involved in the immune regulation of pearl oysters. Our previous research found that nAChR could regulate the inflammatory response induced by transplantation by modulating the apoptosis and proliferation of hemocytes and repairing damaged DNA in pearl oysters ( 24 ). nAChRs belong to a superfamily of pentameric ligand-gated ion channel proteins ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome bisulfite sequencing reveals the function of DNA methylation in the allotransplantation immunity of pearl oysters

Gu,

Yang,

et al. 2023

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

IntroductionIn the pearl culture industry, a major challenge is the overactive immunological response in pearl oysters resulting from allotransplantation, leading to shell-bead rejection and death. To better understand the molecular mechanisms of postoperative recovery and the regulatory role of DNA methylation in gene expression, we analyzed the changes in DNA methylation levels after allotransplantation in pearl oyster Pinctada fucata martensii, and elucidated the regulatory function of DNA methylation in promoter activity of nicotinic acetylcholine receptor (nAChR) gene.MethodsWe constructed nine DNA methylomes at different time points after allotransplantation and used bisulfite genomic sequencing PCR technology (BSP) to verify the methylation status in the promoter of nAChR. We performed Dual luciferase assays to determine the effect of the dense methylation region in the promoter on transcriptional activity and used DNA pull-down and mass spectrometry analysis to assess the capability of transcription factor binding with the dense methylation region.ResultThe DNA methylomes reveal that CG-type methylation is predominant, with a trend opposite to non-CG-type methylation. Promoters, particularly CpG island-rich regions, were less frequently methylated than gene function elements. We identified 5,679 to 7,945 differentially methylated genes (DMGs) in the gene body, and 2,146 to 3,385 DMGs in the promoter at each time point compared to the pre-grafting group. Gene ontology and pathway enrichment analyses showed that these DMGs were mainly associated with “cellular process”, “Membrane”, “Epstein-Barr virus infection”, “Notch signaling pathway”, “Fanconi anemia pathway”, and “Nucleotide excision repair”. Our study also found that the DNA methylation patterns of the promoter region of nAChR gene were consistent with the DNA methylomics data. We further demonstrated that the dense methylation region in the promoter of nAChR affects transcriptional activity, and that the methylation status in the promoter modulates the binding of different transcription factors, particularly transcriptional repressors.ConclusionThese findings enhance our understanding of the immune response and regulation mechanism induced by DNA methylation in pearl oysters after allotransplantation.

show abstract

“…At the first glance, similar to that of other invertebrates, the structure of the existing immune system of bivalves is relatively simple and contains only the innate immune system, lacking an adaptive immune system (Pipe and Coles, 1995). However, accumulating evidence shows that many molecules in bivalves, including acetylcholine (Liu Z. et al, 2016;Cao et al, 2021), biogenic amines (e.g., 5-HT, epinephrine/norepinephrine, and dopamine) (Tiscar and Mosca, 2004;Dong et al, 2017), and amino acid substances (e.g., glutamic acid and γ-aminobutyric acid) (Tiscar and Mosca, 2004;Li et al, 2016), function in regulating their immune response to pathogenic bacteria, viruses, or mechanical damage.…”

Section: Introductionmentioning

confidence: 99%

Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii

et al. 2021

Self Cite

View full text Add to dashboard Cite

The polyamine putrescine (Put) is a ubiquitous small cationic amine. It plays an essential role in controlling the innate immune response. However, little is known about its function in mollusks. In this study, the Put content was observed to increase in the serum of pearl oyster Pinctada fucata martensii after 6 and 24 h of lipopolysaccharide (LPS) stimulation. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) increased, and nitric oxide synthase was downregulated in the Put group (i.e., combined treatment with Put and LPS) compared with that in the LPS group (i.e., combined treatment with phosphate-buffered saline and LPS). Furthermore, activities of alkaline phosphatase and acid phosphatase were inhibited after 6 h of LPS stimulation. The expression levels of the nuclear factor kappa B, IκB kinase, Janus kinase, and signal transducer and activator of transcription proteins genes were all significantly suppressed at 12 and 24 h in the Put group. Pseudomonas aeruginosa and Bacillus subtilis grew better after being incubated with the serum from the Put group than that from the LPS group. Additionally, the Put treatment remarkably inhibited the autophagy of hemocytes mediated by the AMP-activated protein kinase-mammalian target of rapamycin-Beclin-1 pathway. This study demonstrated that Put can effectively inhibit the inflammatory response induced by LPS in pearl oysters. These results provide useful information for further exploration of the immunoregulatory functions of polyamines in bivalves and contribute to the development of immunosuppressive agents.

show abstract

Transcriptome analysis provides insights into the function of the cholinergic system in pearl oyster transplantation immunity

Cited by 9 publications

References 39 publications

Lipidomic insights into the immune response and pearl formation in transplanted pearl oyster Pinctada fucata martensii

Lipidomic insights into the immune response and pearl formation in transplanted pearl oyster Pinctada fucata martensii

Whole-genome bisulfite sequencing reveals the function of DNA methylation in the allotransplantation immunity of pearl oysters

Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii

Contact Info

Product

Resources

About