Transcriptome analysis revealed bisphenol A and nonylphenol affect reproduction

Tanaka, Tomoaki; Ono, Yuriko; Hikihara, Naoki; Yoshida, Ayana; Yamada, Hasumi; Higaki, Shogo; Nishie, Tomomi; Tooyama, Ikuo; Iida, Kei; Hirasawa, Akira; Takada, Tatsuyuki

doi:10.1016/j.reprotox.2019.06.006

Cited by 11 publications

(8 citation statements)

References 35 publications

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“…Conversely, 58.7% of the genes up-regulated in Usp9X KD are expressed at lower levels in differentiated cells compared to ESC grown in LIF + serum ( Figure 3B). Similar results were obtained using two other gene expression datasets comparing ESC to differentiated cells (NCBI GEO: GSE96809 and GSE124241) (Supplementary Figure S5A) [59,60]. These data indicate that Usp9X KD ESC exhibit stronger repression of genes upregulated during differentiation and higher expression of genes downregulated during differentiation, as observed in the naive or "2i" condition.…”

Section: Usp9x Regulates Pluripotency Transition and Its Depletion Cosupporting

confidence: 82%

“…Expression data from wild-type murine ESC at day 0 and day 8 of differentiation into embryonic bodies were obtained from GSE96809 [59]. Expression data from wild-type D3 ESC at day 0 and day 8 of differentiation into embryonic bodies were obtained from GSE124241 [60]. Expression data from E14 WT ESC derived and maintained in serum or 2i were downloaded from SRR064970 and SRR064972 respectively [61].…”

Section: Expression Profile Of Esc In "2i" Lif+serum In Differentiamentioning

confidence: 99%

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USP9X deubiquitinase couples the pluripotency network and cell metabolism to regulate ESC differentiation potential

Dieuleveult

Salataj

Ransy

et al. 2020

Preprint

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Embryonic stem cells (ESC) have the unique ability to differentiate into all three germ cell layers. ESC transition through different states of pluripotency in response to growth factor signals and environmental cues before becoming terminally differentiated. Here, we demonstrated, by a multi-omic strategy, that the deubiquitinase USP9X regulates the developmental potential of ESC, and their transition from a naive to a more developmentally advance, or primed, state of pluripotency. We show that USP9X facilitates developmental gene expression and induces modifications of the mitochondrial bioenergetics, including decreased routing of pyruvate towards its oxidation and reduced respiration. In addition, USP9X binds to the pluripotency factor ESRRB, regulates its abundance and the transcriptional levels of a subset of its target genes. Finally, under permissive culture conditions, depletion of Usp9X accelerates cell differentiation in all cell lineages. We thus identified a new regulator of naive pluripotency and show that USP9X couples ESRRB pluripotency transcriptional network and cellular metabolism, both of which are important for ESC fate and pluripotency. Words: 164

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Section: Usp9x Regulates Pluripotency Transition and Its Depletion Cosupporting

confidence: 82%

Section: Expression Profile Of Esc In "2i" Lif+serum In Differentiamentioning

confidence: 99%

USP9X deubiquitinase couples the pluripotency network and cell metabolism to regulate ESC differentiation potential

Dieuleveult

Salataj

Ransy

et al. 2020

Preprint

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“…Based on the Loewe additivity (LA) theory and the Bliss independence (BI) theory, nonylphenol (NP) and BPA showed significant synergism in human prostate epithelial cell line RWPE-1 (Gan et al, 2015), indicating combined effects were greater than the mere sum of the individual effects of BPA or NP exposure. Mechanistically, BPA and NP could disrupt the expression of early-meiotic germ cell marker genes and were considered to have a feminizing effect that might be implicated in suppressing male gonadal development (Tanaka et al, 2019). Furthermore, the common exposure to BPA and other EEDs, such as di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP), synergistically disturbed the hormone metabolism to induce testicular toxicity and the occurrence of reproductive dysfunction (Balcı et al, 2020; Baralić et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

BPA-induced prostatic hyperplasia in vitro is correlated with the unbalanced gene expression of AR and ER in the epithelium and stroma

et al. 2021

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As a typical environmental endocrine disruptor (EED), bisphenol A (BPA) can induce pathological hyperplasia of the prostatic epithelium and stroma. This study concentrates mainly on the effect and underlying mechanisms of BPA on prostatic hyperplasia, which is based on the culture of primary human prostate epithelial cells (HPEpiC) and human prostate fibroblasts (HPrF). In an effect to screen the optimal pro-survival BPA levels, HPEpiC and HPrF were, respectively, exposed to concentration gradients of BPA (10−12 M–10−4 M) solution diluted with two corresponding medium and incubated for 72 h at 37°C. CCK-8 assay showed that 10−9 M–10−5 M BPA could facilitate the proliferation of HPEpiC, while similar proliferative effect of HPrF only needed 10−11 M–10−7 M BPA. HPrF were more sensitive to BPA than HPEpiC. The qualification of PCNA gene expression measured using quantitative real-time polymerase chain reaction (qRT-PCR) also mirrored the BPA-induced cell proliferation. Additionally, our results considered that androgen receptor (AR), estrogen receptor (ERα, ERβ), and NFKB1 gene expressions exhibited up-regulation in HPEpiC treated with 10−9 M BPA for 72 h. However, in HPrF, the identical BPA treatment could activate ERα, ERβ, and NFKB1 gene expressions and down-regulated the expression of AR levels. It is further confirmed that low-dose BPA can indeed promote the proliferation of human prostate cells in vitro, and the mechanisms of BPA for prostatic epithelial and stromal hyperplasia may not be consistent.

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“…RNA integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies, Tokyo, Japan). Microarray analysis was performed as described previously . Briefly, genome-wide mRNA expression profiles were obtained by microarray analysis with the Affymetrix GeneChip Human Genome U133 2.0 Array (Affymetrix Santa Clara, CA), in accordance with the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Only probe sets with normalized signals >20 were defined as expressed and selected for analysis. Investigation of the ontology of differentially expressed genes was achieved as previously reported . The significance on the gene expression of individual genes was defined by a value of P < 0.05 in Welch’s ANOVA, comparing the average normalized signal intensities using the Bioconductor package Genefilter.…”

Section: Methodsmentioning

confidence: 99%

Molecular Mechanism of Apoptosis by Amyloid β-Protein Fibrils Formed on Neuronal Cells

Takada

Okubo

Yano

et al. 2020

ACS Chem. Neurosci.

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Aggregational states of amyloid β-protein (Aβ) are critical for its neurotoxicity, although they are not wellcharacterized, particularly after binding to the cell membranes. This is one reason why the mechanisms of Aβ neurotoxicity are controversial and elusive. In this study, the effects of toxic Aβ-(1− 42) fibrils formed in the membrane on cellular processes were investigated using human neuroblastoma SH-SY5Y cells. Consistent with previous observations, fibrillar Aβs formed on the membranes induced activation of caspase-3, the effector caspase for apoptosis. Knockdown analyses of the initiator caspases, caspase-8 and caspase-9, indicated that the apoptosis was induced via activation of caspase-8, followed by activation of caspase-9 and caspase-3. We also found that inflammation signaling pathways including Toll-like receptors and inflammasomes NOD-, LRR-, and pyrin domain-containing protein 3 are involved in the initiation of apoptosis by the Aβ fibrils. These inflammation-related molecules are promising targets for the prevention of apoptotic cell death induced by Aβ.

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Transcriptome analysis revealed bisphenol A and nonylphenol affect reproduction

Cited by 11 publications

References 35 publications

USP9X deubiquitinase couples the pluripotency network and cell metabolism to regulate ESC differentiation potential

USP9X deubiquitinase couples the pluripotency network and cell metabolism to regulate ESC differentiation potential

BPA-induced prostatic hyperplasia in vitro is correlated with the unbalanced gene expression of AR and ER in the epithelium and stroma

Molecular Mechanism of Apoptosis by Amyloid β-Protein Fibrils Formed on Neuronal Cells

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