Transcriptome analysis revealed glucose application affects plant hormone signal transduction pathway in “Red Globe” grape plantlets

Mao, Juan; Li, Wenfang; Mi, Baoqin; Ma, Zhiying; Dawuda, Mohammed Mujitaba; Zuo, Cunwu; Zhang, Yongmei; Jiang, Xuefeng; Chen, Baihong

doi:10.1007/s10725-017-0320-1

Cited by 23 publications

(15 citation statements)

References 42 publications

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“…Soluble sugars are known to be able to transcriptionally regulate a significant number of auxin-related genes. In both herbaceous and woody plants, a rise in sugar (glucose, sucrose) level caused an upregulation of Yucca , PIN and GH3 genes [ 99 , 100 , 101 , 102 , 103 , 104 , 105 ].…”

Section: Discussionmentioning

confidence: 99%

Expression Analysis of Key Auxin Biosynthesis, Transport, and Metabolism Genes of Betula pendula with Special Emphasis on Figured Wood Formation in Karelian Birch

Tarelkina

Novitskaya

Галибина

et al. 2020

Plants

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Auxin status in woody plants is believed to be a critical factor for the quantity and quality of the wood formed. It has been previously demonstrated that figured wood formation in Karelian birch (Betula pendula Roth var. carelica (Merckl.) Hämet-Ahti) is associated with a reduced auxin level and elevated sugar content in the differentiating xylem, but the molecular mechanisms of the abnormal xylogenesis remained largely unclear. We have identified genes involved in auxin biosynthesis (Yucca), polar auxin transport (PIN) and the conjugation of auxin with amino acids (GH3) and UDP-glucose (UGT84B1) in the B. pendula genome, and analysed their expression in trunk tissues of trees differing in wood structure. Almost all the investigated genes were overexpressed in Karelian birch trunks. Although Yucca genes were overexpressed, trunk tissues in areas developing figured grain had traits of an auxin-deficient phenotype. Overexpression of GH3s and UGT84B1 appears to have a greater effect on figured wood formation. Analysis of promoters of the differentially expressed genes revealed a large number of binding sites with various transcription factors associated with auxin and sugar signalling. These data agree with the hypothesis that anomalous figured wood formation in Karelian birch may be associated with the sugar induction of auxin conjugation.

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Section: Discussionmentioning

confidence: 99%

Expression Analysis of Key Auxin Biosynthesis, Transport, and Metabolism Genes of Betula pendula with Special Emphasis on Figured Wood Formation in Karelian Birch

Tarelkina

Novitskaya

Галибина

et al. 2020

Plants

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“…To select DNA fragments of preferentially 150–200 bp in length, library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, MA, USA). Other details for the process of library construction was described in Mao et al (2018), and the 10 libraries were sequenced on an Illumina HiSeq 2000 platform.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of light-inducible and light-independent anthocyanins regulated by specific genes in grape ‘Marselan’ (V. viniferaL.)

Mao

et al. 2019

PeerJ

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Anthocyanin is an important parameter for evaluating the quality of wine grapes. However, the effects of different light intensities on anthocyanin synthesis in grape berry skin and its regulation mechanisms are still unclear. In this experiment, clusters of wine grape cv. ‘Marselan’ were bagged using fruit bags with different light transmittance of 50%, 15%, 5%, and 0, designated as treatment A, B, C and D, respectively. Fruits that were not bagged were used as the control, designated as CK. The anthocyanin composition and concentration, as well as gene expression profiles in the berry skin were determined. The results showed that the degree of coloration of the berry skin reduced with the decrease of the light transmittance, and the veraison was postponed for 10 days in D when compared with the CK. Total anthocyanin concentration in the berry skin treated with D decreased by 51.50% compared with CK at the harvest stage. A total of 24 and 21 anthocyanins were detected in CK and D, respectively. Among them, Malvidin-3-O-coumaroylglucoside (trans), which showed a significant positive correlation with the total concentration of anthocyanins at the harvest stage (r = 0.775) and was not detected in D, was presumed to be light-induced anthocyanin. Other anthocyanins which were both synthesized in CK and D were considered to be light-independent anthocyanins. Among them, Malvidin-3-O-coumaroylglucoside (cis) and Malvidin-3-O-acetylglucoside were typical representatives. Remarkably, the synthesis of light-inducible anthocyanins and light-independent anthocyanins were regulated by different candidate structural genes involved in flavonoid biosynthesis pathway and members of MYB and bHLH transcription factors.

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“…The apparatus used for high-performance liquid chromatography (HPLC) was the LC-20AD system (Shimadzu, Kyoto, Japan) equipped with a Zorbax Eclipse Plus C18 column (4.6 mm × 250 mm × 5.0 μm, Agilent, Palo Alto, CA, USA) and an SPD-20A UV detector. The determination method was performed with different concentrations of IAA, GA, and ABA, standard samples, which were used to construct a standard curve [27].…”

Section: Methodsmentioning

confidence: 99%

“…The 5 triplicate samples (5 varieties at S2) yielded 15 nondirectional cDNA libraries with a total of 68.18 million reads (Table 1), which were prepared from 3.0 μg of total RNA using the NEBNext, Ultra TM RNA Library Prep Kit (NEB, USA). The process of library construction was described in detail by Mao et al (2017) [26, 27], and the 15 libraries were sequenced on an Illumina HiSeq 2000 platform.…”

Section: Methodsmentioning

confidence: 99%

“…Three biological and three technical replicates for each reaction were analysed on a LightCycler® 96 SW 1.1 instrument (Roche). The amplification program was described in detail by Mao et al (2017) [27]. All relative expression was normalized by comparing with G0 expression and analysed using the comparative 2 -ΔΔC T method [30] against the reference gene MdGADPH .…”

Section: Methodsmentioning

confidence: 99%

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Anthocyanin accumulation correlates with hormones in the fruit skin of ‘Red Delicious’ and its four generation bud sport mutants

Mao

Yang

et al. 2018

BMC Plant Biol

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BackgroundBud sport mutants of apple (Malus domestica Borkh.) trees with a highly blushed colouring pattern are mainly caused by the accumulation of anthocyanins in the fruit skin. Hormones are important factors modulating anthocyanin accumulation. However, a good understanding of the interplay between hormones and anthocyanin synthesis in apples, especially in mutants at the molecular level, remains elusive. Here, physiological and comparative transcriptome approaches were used to reveal the molecular basis of color pigmentation in the skin of ‘Red Delicious’ (G0) and its mutants, including ‘Starking Red’ (G1), ‘Starkrimson’ (G2), ‘Campbell Redchief’ (G3) and ‘Vallee spur’ (G4).ResultsPigmentation in the skin gradually proliferated from G0 to G4. The anthocyanin content was higher in the mutants than in ‘Red Delicious’. The activation of early phenylpropanoid biosynthesis genes, including ASP3, PAL, 4CL, PER, CHS, CYP98A and F3’H, was more responsible for anthocyanin accumulation in mutants at the color break stage. In addition, IAA and ABA had a positive regulatory effect on the synthesis of anthocyanins, while GA had the reverse effect. The down-regulation of AACT1, HMGS, HMGR, MVK, MVD2, IDI1 and FPPS2 involved in terpenoid biosynthesis influences anthocyanin accumulation by positively regulating transcripts of AUX1 and SAUR that contribute to the synthesis of IAA, GID2 to GA, PP2C and SnRK2 to ABA. Furthermore, MYB and bHLH members, which are highly correlated (r=0.882–0.980) with anthocyanin content, modulated anthocyanin accumulation by regulating the transcription of structural genes, including CHS and F3’H, involved in the flavonoid biosynthesis pathway.ConclusionsThe present comprehensive transcriptome analyses contribute to the understanding of the the relationship between hormones and anthocyanin synthesis as well as the molecular mechanism involved in apple skin pigmentation.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1595-8) contains supplementary material, which is available to authorized users.

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Transcriptome analysis revealed glucose application affects plant hormone signal transduction pathway in “Red Globe” grape plantlets

Cited by 23 publications

References 42 publications

Expression Analysis of Key Auxin Biosynthesis, Transport, and Metabolism Genes of Betula pendula with Special Emphasis on Figured Wood Formation in Karelian Birch

Expression Analysis of Key Auxin Biosynthesis, Transport, and Metabolism Genes of Betula pendula with Special Emphasis on Figured Wood Formation in Karelian Birch

Synthesis of light-inducible and light-independent anthocyanins regulated by specific genes in grape ‘Marselan’ (V. viniferaL.)

Anthocyanin accumulation correlates with hormones in the fruit skin of ‘Red Delicious’ and its four generation bud sport mutants

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