Transcriptome analysis reveals gene expression changes of the basidiomycetous yeast Apiotrichum mycotoxinivorans in response to ochratoxin A exposure

Yang, Zhikai; Huang, Xueling; Peng, Liang

doi:10.1016/j.ecoenv.2022.114146

Cited by 7 publications

(2 citation statements)

References 90 publications

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“…GO analysis revealed that exposure to AFB 1 affects various aspects of B. amyloliquefaciens , including growth, metabolism, reproduction, and cellular activity. These changes are closely related to the function of organelles and cell membranes . Typically, AFB 1 exerts toxic effects by interfering with cellular metabolic processes and may directly or indirectly damage the ribosome, thereby affecting protein synthesis .…”

Section: Discussionmentioning

confidence: 99%

Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Shi,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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Aflatoxin B 1 (AFB 1 ), a mycotoxin and natural carcinogen, commonly contaminates cereals and animal feeds, posing serious health risks to human and animal. In this study, Bacillus amyloliquefaciens ZG08 isolated from kimchi could effectively remove 80.93% of AFB 1 within 72 h at 37 °C and pH 7.0. Metabolome and transcriptome analysis showed that metabolic processes including glycerophospholipid metabolism and amino acid metabolism were most affected in B. amyloliquefaciens ZG08 exposed to AFB 1 . The adaptation mechanism likely involved activation of the thioredoxin system to restore intracellular redox equilibrium. The key genes, tpx and gldA, overexpressed in Escherichia coli BL21, achieved degradation rates of 60.15% and 47.16% for 100 μg/kg AFB 1 under optimal conditions of 37 °C and pH 8.0 and 45 °C and pH 7.0, respectively. The degradation products, identified as AFD 1 , were less cytotoxic than AFB 1 in HepG2 cells. These findings suggest potential strategies for utilizing probiotics and engineered enzymes in AFB 1 detoxification.

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Section: Discussionmentioning

confidence: 99%

Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Shi,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…Carbohydrate metabolisms could provide the primary energy and substrate source, and are the most important metabolic pathways for biodegradation of organic pollutants in organism (Meng et al 2019;Liu et al 2021). Additionally, the processes of amino acid transport and metabolism, secondary metabolisms, membrane transport might also play an important role in the biodegradation behavior (Yang et al 2022). This nding is in agreement with the biodegradation of Pseudomonas brassicacearum strain MPDS to polycyclic aromatic hydrocarbons (Liu et al 2021) and chlorantraniliprole detoxi cation in Chilo suppressalis (Meng et al 2019).…”

Section: Function Classi Cation Annotation and Enrichment Analysis Of...mentioning

confidence: 99%

Characterization and transcriptome analysis of triadimefon-degrading strain Enterobacter hormaechei TY18

Wang

Guan

Zhao

et al. 2023

Preprint

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Triadimefon, a type of triazole systemic fungicide, has been extensively used to control various fungal diseases. However, extensive application of triadimefon contributes to austere environmental pollution, and even threatens human health. In our study, Enterobacter hormaechei strain TY18 could grew well in carbon salt medium (CSM) with triadimefon as sole nitrogen source, and could significantlydegrade triadimefon. Under triadimefon stress, a total of 430 differentially expressed genes (DEGs), including 197 up-regulated and 233 down-regulated DEGs, were found in strain TY18 from transcriptome sequencing (RNA-Seq). Functional classification and enrichment analysis revealed that these DEGs were mainly related to amino acid transport and metabolism, carbohydrate transport and metabolism, small molecule and pyrimidine metabolism. Interestingly, the DEGs encoding monooxygenase and hydrolase activity acting on carbon-nitrogen were highly up-regulated, might be mainly responsible for the metabolism in triadimefon. The finding in this work suggested that the novel strain E. hormaechei TY18 could degrade efficiently triadimefon for the first time, and contribute to further understanding of the degradation mechanism related to triadimefon in strain TY18.

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Biotransformation activities of fungal strain apiotrichum sp. IB-1 to ibuprofen and naproxen

Peng,

Yun,

et al. 2024

Arch Microbiol

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Transcriptome analysis reveals gene expression changes of the basidiomycetous yeast Apiotrichum mycotoxinivorans in response to ochratoxin A exposure

Cited by 7 publications

References 90 publications

Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Characterization and transcriptome analysis of triadimefon-degrading strain Enterobacter hormaechei TY18

Biotransformation activities of fungal strain apiotrichum sp. IB-1 to ibuprofen and naproxen

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Transcriptome analysis reveals gene expression changes of the basidiomycetous yeast Apiotrichum mycotoxinivorans in response to ochratoxin A exposure

Cited by 7 publications

References 90 publications

Biodegradation Characteristics and Mechanism of Aflatoxin B1 by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Biodegradation Characteristics and Mechanism of Aflatoxin B1 by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Characterization and transcriptome analysis of triadimefon-degrading strain Enterobacter hormaechei TY18

Biotransformation activities of fungal strain apiotrichum sp. IB-1 to ibuprofen and naproxen

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Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

Biodegradation Characteristics and Mechanism of Aflatoxin B₁ by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives