Transcriptome Analysis Reveals Multiple Genes and Complex Hormonal-Mediated Interactions with PEG during Adventitious Root Formation in Apple

Li, Shaohuan; Tahir, Muhammad Mobeen; Wu, Tong; Xie, Lingling; Zhang, Xiaoyun; Mao, Jiangping; Ayyoub, Anam; Xing, Libo; Zhang, Dong; Shao, Yun

doi:10.3390/ijms23020976

Cited by 14 publications

(2 citation statements)

References 63 publications

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“…In addition, the levels of GA3 were significantly higher in Garnem than in GF 677. A recent transcriptomic study on AR formation in apple rootstocks suggested that higher ABA and MeJA accumulation and lower GA3 levels could lead to AR inhibition [ 36 ]. Based on these results, it is tempting to speculate that a conserved hormonal balance could regulate AR formation in woody species and, as such, could be used as a bioindicator of rooting performance during rootstock breeding.…”

Section: Discussionmentioning

confidence: 99%

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Justamante

Mhimdi

Molina-Pérez

et al. 2022

Plants

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Several Prunus species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relevance for stress tolerance, which has led to the development of environmentally resilient plants. As a first step towards understanding the genetic determinants involved in AR formation in Prunus sp., we evaluated the rooting of hardwood cuttings from five Prunus rootstocks (Adafuel, Adarcias, Cadaman, Garnem, and GF 677) grown in hydroponics. We found that auxin-induced callus and rooting responses were strongly genotype-dependent. To investigate the molecular mechanisms involved in these differential responses, we performed a time-series study of AR formation in two rootstocks with contrasting rooting performance, Garnem and GF 677, by culturing in vitro microcuttings with and without auxin treatment (0.9 mg/L of indole-3-butyric acid [IBA]). Despite showing a similar histological structure, Garnem and GF677 rootstocks displayed dynamic changes in endogenous hormone homeostasis involving metabolites such as indole-3-acetic acid (IAA) conjugated to aspartic acid (IAA-Asp), and these changes could explain the differences observed during rooting.

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Section: Discussionmentioning

confidence: 99%

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Justamante

Mhimdi

Molina-Pérez

et al. 2022

Plants

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“…Furthermore, sugar content regulated the process of AR formation and sucrose AR numbers in apples (Pawlicki & Welander, 1995). In recent studies, starch and sucrose metabolism-related genes were activated by KCl and indole butyric acid treatments and promoted AR formation; in contrast, these were inhibited by drought and high nitrate stresses and led to severe defects in AR formation in apples (Li et al, 2018(Li et al, , 2022cTahir et al, 2021a;Zhang et al, 2021). Consistent with these observations, starch and sucrose metabolism-related genes were upregulated in WT_LN_vs_OE_LN and downregulated in OE_LN_v-s_OE_MN and WT_LN_vs_WT_MN microshoots (Supporting Information: Figure S14 and Table S12), which were highly correlated with their phenotypes (Figure 1).…”

Section: Sugar Signaling Mediated Ar Formationmentioning

confidence: 99%

Insights into the complicated networks contribute to adventitious rooting in transgenic MdWOX11 apple microshoots under nitrate treatments

Tahir

Tong

Fan

et al. 2022

Plant Cell & Environment

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Adventitious root formation is a bottleneck for the mass propagation of microshoots, and nitrate is an essential nutrient regulating adventitious roots. WOX11 is involved in adventitious rooting. But the crosstalk between nitrate and WOX11 is completely unknown. In this study, MdWOX11 transgenic apple microshoots were grown on different nitrate treatments. Low nitrate promotes adventitious rooting in overexpressed microshoots more than wild type and RNA interference microshoots. In contrast, medium nitrate significantly inhibits it in overexpressed and RNA interference microshoots compared with wild type microshoots. Stem anatomy indicated that medium nitrate delays root primordia formation compared with low nitrate. Methyl jasmonate and zeatin riboside played positive and negative roles in adventitious rooting, respectively. Transcriptomic analysis was conducted to understand the molecular mechanisms behind the phenotypes better. Hormone signalling, sugar metabolism, nitrogen metabolism, cell cycle and root development pathway‐related genes were selected for their potential involvement in adventitious rooting. Results suggest that nitrogen signaling and MdWOX11 expression affect cytokinin accumulation and response to cytokinin through regulating the expression of genes related to cytokinin synthesis and transduction pathways, which ultimately affect adventitious rooting. This study provided important insights into the complicated networks involved in adventitious rooting in transgenic microshoots under nitrate treatments.

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Stock plant etiolation reduces rooting of sub-terminal olive cuttings by reducing total sugars, IAA, indole/phenol ratio, and IAA/GA ratio

Abdel-Mohsen

Rashedy

2023

Acta Physiol Plant

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Adventitious root formation is the key to successful propagation of olives through cuttings. Etiolation, as an eco-friendly practice, can modulate the plant’s endogenous phytohormone, which plays an important role in stimulation of rooting of cuttings. Therefore, this study was aimed to investigate the effect of etiolation on the rooting behavior of both ‘Manzanillo’ and ‘Picual’ olive cuttings (terminal and sub-terminal) treated with IBA (0, 4000 ppm). The results indicated that sub-terminal cuttings in both cultivars were more effective in olive propagation than terminal cuttings as they recorded increased leaves number, C/N ratio, total carbohydrates, total indoles, IAA, IAA/GA ratio, and total phenols. Etiolation of both olive cultivars for both terminal and sub-terminal cuttings significantly decreased rooting percent, roots number, IAA, and IAA/GA ratio; while, it increased GA and GA/IAA. Moreover, etiolation decreased total sugars, IAA, IAA/GA ratio, and indole/phenol ratio in both sub-terminal cuttings. Terminal ‘Manzanillo’ cuttings achieved a higher rooting percent than terminal ‘Picual’ cuttings by 5.83% and 202.2% as mean of both seasons for untreated and IBA-treated cuttings, which was accompanied by significant increase in vascular bundle%, total phenol, phenol/indole ratio, IAA, and IAA/GA ratio by 21.48%, 49.2%, 44.72%, 12.9%, and 22.9%, respectively. Also, terminal ‘Manzanillo’ cuttings recorded lower GA by 7.98% and GA/IAA ratio by 18.21% and pith by 6.13% than terminal ‘Picual’ cuttings. This study proved that olive plants need exposure to full sunlight to propagate easily by cuttings since etiolation had negative effects on IBA-treated cuttings.

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Transcriptome Analysis Reveals Multiple Genes and Complex Hormonal-Mediated Interactions with PEG during Adventitious Root Formation in Apple

Cited by 14 publications

References 63 publications

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Insights into the complicated networks contribute to adventitious rooting in transgenic MdWOX11 apple microshoots under nitrate treatments

Stock plant etiolation reduces rooting of sub-terminal olive cuttings by reducing total sugars, IAA, indole/phenol ratio, and IAA/GA ratio

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