Transcriptome Analysis Reveals the Negative Effect of 16 T High Static Magnetic Field on Osteoclastogenesis of RAW264.7 Cells

Abstract

The magnetic field is the most common element in the universe, and high static magnetic field (HiSMF) has been reported to act as an inhibited factor for osteoclasts differentiation. Although many studies have indicated the negative role of HiSMF on osteoclastogenesis of RANKL-induced RAW264.7 cells, the molecular mechanism is still elusive. In this study, the HiSMF-retarded cycle and weakened differentiation of RAW264.7 cells was identified. Through RNA-seq analysis, RANKL-induced RAW264.7 cells under HiSMF were analysed, and a total number of 197 differentially expressed genes (DEGs) were discovered. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that regulators of cell cycle and cell division such as Bub1b, Rbl1, Ube2c, Kif11, and Nusap1 were highly expressed, and CtsK, the marker gene of osteoclastogenesis was downregulated in HiSMF group. In addition, pathways related to DNA replication, cell cycle, and metabolic pathways were significantly inhibited in the HiSMF group compared to the Control group. Collectively, this study describes the negative changes occurring throughout osteoclastogenesis under 16 T HiSMF treatment from the morphological and molecular perspectives. Our study provides information that may be utilized in improving magnetotherapy on bone disease.

A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis

“…Piezo1 is embedded in the cell membrane, and it converts extracellular mechanical stimuli into intracellular biochemical signals [ 37 ]. MSCs are mechanosensitive [ 38 ], and SMF provides biophysical stimuli [ 39 ]. In our study, SMF increased Piezo1 in the BMSCs and transformed mechanical signals into biological signals in vivo .…”

Exploration of Gene Expression Characters of Mouse M-1 Cells Treated by Qigong

“…In another study, through RNA-seq analysis, RANKL-induced RAW264.7 cells under high static magnetic field were analyzed. As the result, a total number of 197 DEGs were discovered and the related molecular mechanism was discussed [35]. Therefore, transcriptome analysis was a very sensitive assay method for weak treatment such as electromagnetic radiation, magnetic field, Qigong, etc.…”