Transcriptome and DNA methylome provide insights into the molecular regulation of drought stress in sea buckthorn

Lyu, Zhongrui; Zhang, Guoyun; Song, Yating; He, Caiyun; Jian-guo, Zhang

doi:10.1016/j.ygeno.2022.110345

Cited by 10 publications

(6 citation statements)

References 60 publications

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“…A growing body of research indicates that modifications to the levels of secondary metabolites accumulated [ 29 – 30 ] and DNA methylation status of the genome and functional genes [ 31 – 32 ] are essential for plant adaptation to drought stress. Uncertainty exists regarding the precise method by which DNA methylation controls plant secondary metabolism during drought.…”

Section: Discussionmentioning

confidence: 99%

DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress

Wang,

Zhao,

et al. 2024

BMC Genomics

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Plant growth and development can be significantly impacted by drought stress. Plants will adjust the synthesis and accumulation of secondary metabolites to improve survival in times of water constraint. Simultaneously, drought stress can lead to modifications in the DNA methylation status of plants, and these modifications can directly impact gene expression and product synthesis by changing the DNA methylation status of functional genes involved in secondary metabolite synthesis. However, further research is needed to fully understand the extent to which DNA methylation modifies the content of secondary metabolites to mediate plants’ responses to drought stress, as well as the underlying mechanisms involved. Our study found that in Eleutherococcus senticosus (E. senticosus), moderate water deprivation significantly decreased DNA methylation levels throughout the genome and at the promoters of EsFPS, EsSS, and EsSE. Transcription factors like EsMYB-r1, previously inhibited by DNA methylation, can re-bind to the EsFPS promotor region following DNA demethylation. This process promotes gene expression and, ultimately, saponin synthesis and accumulation. The increased saponin levels in E. senticosus acted as antioxidants, enhancing the plant’s adaptability to drought stress.

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Section: Discussionmentioning

confidence: 99%

DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress

Wang,

Zhao,

et al. 2024

BMC Genomics

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“…There are few circRNA studies in plants compared to research reports in animals. circRNAs related to drought stress have been reported in plants such as Pyrus betulifolia Bunge, apple, sea buckthorn, Zea mays, Alfalfa (Medicago sativa L.), and Phyllostachys aureosulcata [23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Whole-Transcriptome Sequencing Reveals the Global Molecular Responses and NAC Transcription Factors Involved in Drought Stress in Dendrobium catenatum

Zhang,

Han,

Zeng

et al. 2024

Antioxidants

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Dendrobium catenatum is a highly drought-tolerant herb, which usually grows on cliffs or in the branches of trees, yet the underlying molecular mechanisms for its tolerance remain poorly understood. We conducted a comprehensive study utilizing whole-transcriptome sequencing approaches to investigate the molecular response to extreme drought stress in D. catenatum. A large number of differentially expressed mRNAs, lncRNAs, and circRNAs have been identified, and the NAC transcription factor family was highly enriched. Meanwhile, 46 genes were significantly up-regulated in the ABA-activated signaling pathway. In addition to the 89 NAC family members accurately identified in this study, 32 members were found to have different expressions between the CK and extreme drought treatment. They may regulate drought stress through both ABA-dependent and ABA-independent pathways. Moreover, the 32 analyzed differentially expressed DcNACs were found to be predominantly expressed in the floral organs and roots. The ceRNA regulatory network showed that DcNAC87 is at the core of the ceRNA network and is regulated by miR169, miR393, and four lncRNAs. These investigations provided valuable information on the role of NAC transcription factors in D. catenatum’s response to drought stress.

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“…Although sea buckthorn has evolved a variety of drought‐tolerant structural properties and physiological mechanisms, water is still the main limiting factor for growth. Currently, researchers have conducted extensive studies on morphological anatomy (Li et al 2007), ecological characteristics (Xu et al 2008), water physiology and biochemistry (Yang et al 2005) and gene/protein expression (Lyu et al 2022) under drought stress, but the molecular regulatory mechanisms of drought tolerance in sea buckthorn are not clear.…”

Section: Introductionmentioning

confidence: 99%

Histone H3K9 acetylation modulates gene expression of key enzymes in the flavonoid and abscisic acid pathways and enhances drought resistance of sea buckthorn

Li,

Wei,

Song

et al. 2023

Physiologia Plantarum

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The effect of histone H3K9 acetylation modification on gene expression and drought resistance in drought‐resistant tree species is not clear. Using the chromatin immunoprecipitation (ChIP) method, this study obtained nine H3K9 acetylated protein‐interacting DNAs from sea buckthorn seedlings, and the ChIP sequencing result predicted about 56,591, 2217 and 5119 enriched region peaks in the control, drought and rehydration comparative groups, respectively. Gene functional analysis of differential peaks from three comparison groups revealed that 105 pathways were involved in the drought resistance process, and 474 genes were enriched in the plant hormone signaling transduction pathways. Combined ChIP‐seq and transcriptome analysis revealed that six genes related to abscisic acid synthesis and signaling pathways, 17 genes involved in flavonoid biosynthesis, and 15 genes involved in carotenoid biosynthesis were positively regulated by H3K9 acetylation modification under drought stress. Under drought stress conditions, the content of abscisic acid and the expression of related genes were significantly up‐regulated, while the content of flavonoids and the expression of key enzymes involved in their synthesis were largely down‐regulated. Meanwhile, after exposure to histone deacetylase inhibitors (trichostatin A), the change of abscisic acid and flavonoids content and their related gene expression were slowed down under drought stress. This study will provide an important theoretical basis for understanding the regulatory mechanisms of histone acetylation modifications in sea buckthorn drought resistance.

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Transcriptome and DNA methylome provide insights into the molecular regulation of drought stress in sea buckthorn

Cited by 10 publications

References 60 publications

DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress

DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress

Whole-Transcriptome Sequencing Reveals the Global Molecular Responses and NAC Transcription Factors Involved in Drought Stress in Dendrobium catenatum

Histone H3K9 acetylation modulates gene expression of key enzymes in the flavonoid and abscisic acid pathways and enhances drought resistance of sea buckthorn

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