Transcriptome and enzyme activity analyses of tolerance mechanisms in pearl oyster (Pinctada fucata) under high-temperature stress

Zhang, Hua; Jia, Huixia; Xiong, Ping; Yao, Gaoyou; He, Maoxian

doi:10.1016/j.aquaculture.2022.737888

Cited by 29 publications

(6 citation statements)

References 52 publications

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“…A high temperature greater than 57 °C was not conducted, and the optimum temperature for the bromelain is around 55 °C [ 23 ]. A high temperature may inactivate the enzyme, owing to the unfolding of its protein structure and thermal denaturation [ 24 ]. There were no significant differences for the color degradation rates when the temperature was ranging from 27 to 47 °C ( p > 0.05), and the color degradation rate reached 11.23 ± 0.22% at 57 °C, indicating that the color of PEH was seriously damaged under high temperature conditions.…”

Section: Resultsmentioning

confidence: 99%

The Hydrolysis of Pigment-Protein Phycoerythrin by Bromelain Enhances the Color Stability

Sun

Cui

Wang

et al. 2023

Foods

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Phycoerythrin (PE) is a natural protein–pigment complex with a strong pink color, but it is sensitive to thermal and light variations. In this study, PE was extracted from Porphyra haitanensis in a yield of 0.2% (w/w). The phycoerythrin hydrolysates (PEH) (3–10 kDa) were prepared by enzymatic hydrolysis of PE with bromelain (8000 U/g) at 47 °C for 30 min, with a degree of hydrolysis (DH) of 11.57 ± 0.39% and a color degradation rate of 7.98 ± 0.39%. The physicochemical properties of PEH were evaluated. The UV and fluorescence spectra indicated that bromelain changed the microenvironment around phycoerythrobilin (PEB). The infrared spectrum revealed that the bromelain hydrolysis increased the α-helix content of PEH. The scanning electron microscope showed that bromelain destroyed the dense and smooth structure of PE, resulting in irregular porous structures. The radical scavenging activities of DPPH and ABTS of PEH were increased relative to that of PE (p < 0.05). The thermal (40–80 °C)-, UV (0.5–3 h)-, visible light irradiation (2–8 h)-, and metal ion exposing stabilities of PEH were significantly improved (p < 0.05). This study provides a potential scheme for overcoming the sensitivity of PE to thermal and light variations and facilitates PEH as a natural colorant ingredient in food and pigment applications.

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Section: Resultsmentioning

confidence: 99%

The Hydrolysis of Pigment-Protein Phycoerythrin by Bromelain Enhances the Color Stability

Sun

Cui

Wang

et al. 2023

Foods

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“…High levels of lactic acid indicate high levels of anaerobic metabolism [45]. Under heat stress, LD or LDH levels in olive flounder Paralichthys olivaceus, turbot Scophthalmus maximus, brook trout Salvelinus fontinalis, and pearl oyster Pinctada fucata are elevated, and anaerobic respiration is enhanced [46][47][48]. In the present study, the LD and LDH levels of large spotted seabass increased from 0 h to 6 h after heat stress at 32 • C. This suggests that the aerobic metabolism of large spotted seabass decreased within 6 h after heat stress, while the anaerobic metabolism was enhanced to maintain the body's energy supply.…”

Section: Discussionmentioning

confidence: 99%

“…The LD content of the two sizes of spotted seabass decreased significantly after 12 h, which may indicate that heat stress accelerated the sugar production rate to help fish restore their energy storage to the pre-stress level. LDH is an essential enzyme for energy metabolism in the body, which catalyzes the conversion between pyruvate and lactic acid depending on substrate concentration [48]. The LDH content of small spotted seabass increased after 24 h but LD did not increase, which may be related to the rate of pyruvate generation.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Physiological Responses to Heat Stress of Two Sizes of Juvenile Spotted Seabass (Lateolabrax maculatus)

Qin

Long

Huang

et al. 2023

Fishes

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Temperature affects the metabolism of fish, and fish of different sizes have different tolerances to temperature. The aim of this experiment was to compare two sizes of juvenile spotted seabass, Lateolabrax maculatus (with average weights of 57.91 ± 11.57 g and 13.92 ± 2.77 g, respectively) for changes in physiological, biochemical, and molecular mechanisms under acute heat stress. Experimental fish were exposed to acute temperature increasing from 23 °C to 32 °C, and the mortality rate was noted at various heat stress exposures (0, 3, 6, 12, 24, 48, and 72 h). Moreover, serum and liver were obtained before and after heat stress. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase (SOD), malondialdehyde (MDA), lactic acid (LD), lactate dehydrogenase (LDH), glucose, and hepatic glycogen, and the expression of heat shock proteins (HSP70, HSP90) and apoptosis-related genes (BAX, caspase-3) in two sizes of spotted seabass were measured. Results showed that the contents of AST, ALT, SOD, MDA, LD, and glucose as well as the expression level of BAX and mortality were higher in large spotted seabass than in small spotted seabass within 12 h. These results indicate that the large spotted seabass had higher levels of oxidative stress and more severe liver damage, resulting in a higher mortality. Furthermore, the HSPs expression level of small spotted seabass was higher and the mortality was lower than that of large spotted seabass. Therefore, we considered that the large spotted seabass has lower levels of HSPs expression, causing their physiological response to be elevated to resist heat stress. In conclusion, spotted seabass with larger size has a poorer tolerance to heat stress compared with spotted seabass with smaller size. The smaller fish size was possibly resistant to heat stress by regulating the HSPs expression level in a more active extent.

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“…qRT-PCR was conducted in a 20µl volume containing 1µl of diluted cDNAs, 0.4µl of the each primer(showed in table S6), 0.4µL of passive reference dye, 7.8µl H 2 O, and 10 µL of Top Green qPCR SuperMix. Thermal cycling involved heating at 95 •C for 300 s, followed by 40 cycles of 95 •C for 15 s, 60 •C for 30 s and 72 •C for 30 s. The MS Actin gene was used as an internal control, and the relative expression levels were calculated by the comparative 2 −△△Ct method [34].…”

Section: Rna Extraction and Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

Comparative Transcriptomaic and Metabolomic analyses provide insights into the responses to High Temperature Stress in Alfalfa (Medicago satave.L)

Zhou,

Tang,

et al. 2024

Preprint

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High temperature stress (HTS) is one of the most severe forms of abiotic stress in alfalfa, With the intensification of climate change, the frequency of high temperature stress will further increase in the future, which will bring challenges to the growth and development of alfalfa. Therefore, untargeted metabolomic and RNA-Seq profiling were implemented to unravel the possible alteration in alfalfa seedlings subjected to different temperature stress (25℃, 30℃, 35℃, 40℃) in this study. Results revealed that HTS significantly altered some pivotal transcripts and metabolites. The number of differentially expressed genes (DEGs) markedly up- and down-regulated was 1876 and1524 in T30_vs_CK, 2815 and 2667 in T35_vs_CK, and 2115 and 2226 in T40_vs_CK, respectively. The number for significantly up-regulated and down-regulated differential metabolites was 173 and 73 in T30_vs_CK, 188 and 57 in T35_vs_CK, and 220 and 66 in T40_vs_CK, respectively. It is worth noting that metabolomics and transcriptomics co-analysis characterized enriched in plant hormone signal transduction(ko04705), glyoxylate and dicarboxylate metabolism (ko00630), from which some differentially expressed genes and differential metabolites participated. In particular, the content of hormone changed significantly under T40 stress, suggesting that maintaining normal hormone synthesis and metabolism may be an important way to improve the HTS tolerance of alfalfa. qRT-PCR further showed that the expression pattern was similar to the expression abundance in the transcriptome. This study provides a practical and in-depth perspective from transcriptomics and metabolomics in investigating the effects conferred by temperature on plant growth and development, which provided the theoretical basis for breeding heat-resistant alfalfa.

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Transcriptome and enzyme activity analyses of tolerance mechanisms in pearl oyster (Pinctada fucata) under high-temperature stress

Cited by 29 publications

References 52 publications

The Hydrolysis of Pigment-Protein Phycoerythrin by Bromelain Enhances the Color Stability

The Hydrolysis of Pigment-Protein Phycoerythrin by Bromelain Enhances the Color Stability

A Comparison of the Physiological Responses to Heat Stress of Two Sizes of Juvenile Spotted Seabass (Lateolabrax maculatus)

Comparative Transcriptomaic and Metabolomic analyses provide insights into the responses to High Temperature Stress in Alfalfa (Medicago satave.L)

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