Transcriptome and miRNAs Profiles Reveal Regulatory Network and Key Regulators of Secondary Xylem Formation in “84K” Poplar

Wang, Huilin; Zhao, Pan; He, Yumei; Su, Yuting; Zhou, Xinyi; Guo, Huihong

doi:10.3390/ijms242216438

Cited by 2 publications

(1 citation statement)

References 63 publications

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“…Poplar 84K (Populus alba × P. glandulosa) has had its entire genome sequenced [36] and tissue culture and genetic transformation are easily performed with this poplar hybrid. Accordingly, Poplar 84K has become a model plant material for forest molecular biology and genetic engineering research [37][38][39]. In this study, a single-cell transcriptome atlas of leaves at different development stages of Poplar 84K was constructed using scRNA-seq data.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptome Atlas of Leaves at Different Developmental Stages in Populus alba × Populus glandulosa Clone 84K

Jing,

Ren,

Zhang

et al. 2024

Forests

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Leaves are crucial photosynthetic plant organs. The development of poplar leaves has spatio-temporal specificity and it is of great significance to study the single-cell transcription atlas of leaves to reveal the temporal regulation of gene expression in different cell types. Here, single-cell RNA sequencing was performed on 17,768 tender leaf and 5846 functional leaf cells of Poplar 84K to construct a transcriptome atlas and developmental trajectory. The results showed that there were five and six cell types in tender and functional leaves, respectively. According to a pseudo-time trajectory analysis and the clustering of expressed genes into different cell types, the development of tender and functional leaves was divided into two temporal stages. Tender leaf epidermal cells developed earliest and were enriched with genes related to cell division and growth, indicating that tender leaves were in the stage of cell expansion and functional differentiation. Functional leaf palisade mesophyll cells were enriched with genes related to photosynthesis and carbon metabolism and cell types performing different functions tended to mature, indicating that functional leaves were in the stage of leaf development and the initial formation of photosynthesis. Our in-depth analysis of the transcriptional regulation at the single-cell level during leaf development provides an important basis for studying the mechanisms involved in cell differentiation and leaf development in poplar as well as other plants.

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Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptome Atlas of Leaves at Different Developmental Stages in Populus alba × Populus glandulosa Clone 84K

Jing,

Ren,

Zhang

et al. 2024

Forests

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Acetylation of transcription factor BpTCP20 by acetyltransferase BpPDCE23 modulates salt tolerance in birch

Liu,

Shi,

Wang

et al. 2024

Plant Physiology

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Teosinte branched 1/Cycloidea/Proliferating cell factor (TCP) transcription factors function in abiotic stress responses. However, how TCPs confer salt tolerance is unclear. Here, we characterized a TCP transcription factor, BpTCP20, that responds to salt stress in birch (Betula platyphylla Suk). Plants overexpressing BpTCP20 displayed increased salt tolerance, and Bptcp20 knockout mutants displayed reduced salt tolerance relative to the wild-type (WT) birch. BpTCP20 conferred salt tolerance by mediating stomatal closure and reducing reactive oxygen species accumulation. Chromatin immunoprecipitation sequencing showed that BpTCP20 binds to NeuroD1, T-box, and two unknown elements (termed TBS1 and TBS2) to regulate target genes. In birch, salt stress led to acetylation of BpTCP20 acetylation at lysine 259. A mutated BpTCP20 variant (abolished for acetylation, termed BpTCP20259) was overexpressed in birch, which led to decreased salt tolerance compared with plants overexpressing BpTCP20. However, BpTCP20259-overexpressing plants still displayed increased salt tolerance relative to untransformed wild-type plants. BpTCP20259 showed reduced binding to the promoters of target genes and decreased target gene activation, leading to decreased salt tolerance. In addition, we identified dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (BpPDCE23), an acetyltransferase that interacts with and acetylates BpTCP20 to enhance its binding to DNA motifs. Together, these results suggest that BpTCP20 is a transcriptional regulator of salt tolerance, whose activity is modulated by BpPDCE23-mediated acetylation.

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Transcriptome and miRNAs Profiles Reveal Regulatory Network and Key Regulators of Secondary Xylem Formation in “84K” Poplar

Cited by 2 publications

References 63 publications

Single-Cell Transcriptome Atlas of Leaves at Different Developmental Stages in Populus alba × Populus glandulosa Clone 84K

Single-Cell Transcriptome Atlas of Leaves at Different Developmental Stages in Populus alba × Populus glandulosa Clone 84K

Acetylation of transcription factor BpTCP20 by acetyltransferase BpPDCE23 modulates salt tolerance in birch

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