Transcriptome and secretome of two Pythium species during infection and saprophytic growth

Caballero, Jorge Ibarra; Tisserat, Ned

doi:10.1016/j.pmpp.2016.09.001

Cited by 5 publications

(5 citation statements)

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“…The presence of genes encoding proteases families such as subtilisin-related proteases, metalloproteases, and E3 ligases, inducing necrosis and cell-wall degradation in the core Pythium spp. genes and the absence or lack of RXLR effectors in Pythium support its non-host specificity and necrotrophic lifestyle [ 117 , 118 , 119 ]. Unlike Phytophthora , Pythium spp.…”

Section: Virulence Mechanism Of Pythium Spp and Challenges In Resistant Breeding Against Pythium Spmentioning

confidence: 99%

“…Unlike Phytophthora , Pythium spp. have been observed to lack RXLR effectors with avirulence activities [ 118 , 119 ]. The absence of these avirulence factors has been correlated with the lack of gene-for-gene resistance against Pythium spp.…”

Section: Virulence Mechanism Of Pythium Spp and Challenges In Resistant Breeding Against Pythium Spmentioning

confidence: 99%

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Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Arora

Sharma

et al. 2021

Microorganisms

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Capsicum annuum L. is a significant horticulture crop known for its pungent varieties and used as a spice. The pungent character in the plant, known as capsaicinoid, has been discovered to have various health benefits. However, its production has been affected due to various exogenous stresses, including diseases caused by a soil-borne pathogen, Pythium spp. predominantly affecting the Capsicum plant in younger stages and causing damping-off, this pathogen can incite root rot in later plant growth stages. Due to the involvement of multiple Pythium spp. and their capability to disperse through various routes, their detection and diagnosis have become crucial. However, the quest for a point-of-care technology is still far from over. The use of an integrated approach with cultural and biological techniques for the management of Pythium spp. can be the best and most sustainable alternative to the traditionally used and hazardous chemical approach. The lack of race-specific resistance genes against Pythium spp. can be compensated with the candidate quantitative trait loci (QTL) genes in C. annuum L. This review will focus on the epidemiological factors playing a major role in disease spread, the currently available diagnostics in species identification, and the management strategies with a special emphasis on Pythium spp. causing damping-off and root rot in different cultivars of C. annuum L.

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Section: Virulence Mechanism Of Pythium Spp and Challenges In Resistant Breeding Against Pythium Spmentioning

confidence: 99%

Section: Virulence Mechanism Of Pythium Spp and Challenges In Resistant Breeding Against Pythium Spmentioning

confidence: 99%

Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Arora

Sharma

et al. 2021

Microorganisms

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“…Resistance to necrotrophic pathogens is generally quantitative (Bruehl 1983;Raajimakers et al 2009). The Pythium genome lacks RxLR effectors, which are known in other oomycete species to be responsible for gene-for-gene resistance interactions with the host plant (Lévesque et al 2010;Caballero and Tisserat 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Elicitins may be important for infection, as they trigger necrosis in host cells (Ponchet et al, 1999). Although elicitins were found to be abundant in P. irregulare and P. iwayamai, the similarity between their elicitins was never more than 70% (Caballero & Tisserat, 2016). Lévesque et al (2010) reported that P. ultimum encodes more E3 ligases than other oomycetes, which are known to degrade host response proteins.…”

Section: Discussionmentioning

confidence: 99%

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Soybean-Pythium pathosystem: Search for host resistance and interaction with Fusarium species

Lerch¹

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Seedling disease of soybean is an economically important disease that reduces crop stands and may reduce yield. Symptoms of seedling disease include pre-and post-emergence damping-off and root rot. Many species of Pythium and Fusarium cause seedling disease and are frequently recovered together from diseased soybean seedlings. Host resistance as a strategy to manage multiple pathogens has only briefly been explored in soybean and the interaction between these pathogens is unclear. To better understand the soybean-Pythium pathosystem, lab and growth chamber assays were conducted to accomplish the following objectives, 1) identify with resistance to seed and root rot caused by multiple Pythium species in the parental lines of the SoyNAM populations, 2) map quantitative trait loci (QTL) contributing to seed rot caused by multiple Pythium species in a SoyNAM population, and 3) evaluate the effect of coinoculation with Pythium and Fusarium species on soybean seedling disease development. A seed and root rot assay was used to evaluate traits associated with seed and root rot. The SoyNAM parents were screened for resistance to Pythium lutarium, P. oopapillum, P. sylvaticum, and P. torulosum. Of the 40 SoyNAM parents, Magellan, Maverick, CLOJ095-4-6, and CLOJ173-6-8 were resistant to P. lutarium, P. sylvaticum, and P. torulosum. The remaining parents were resistant to two, one, or none of the species they were screened against. A correlation analysis was performed to compare seed and root rot severity, and a weak to insignificant correlation was found suggesting that resistance to seed and root rot are not necessarily the same. v The 140 recombinant inbred lines (RILs) of SoyNAM population 29 (PI 427.136 x IA3023) were evaluated for seed rot resistance to P. lutarium, P. oopapillum, and P. sylvaticum. Assessments included seed rot severity (SRS), percent rotted seeds (ROTS), and adjusted germination (GERM). Inclusive composite interval mapping was performed to identify quantitative trait loci (QTL) contributing to seed rot resistance to three Pythium species. When the log of the odds (LOD) threshold was set using 1000 permutations, no QTL were identified for resistance. When the LOD was manually set at 3, several QTL were identified for resistance to each Pythium species, however the explained phenotypic variance (PVE) for all QTL was below 2.3%. Further screening with a modified assay may be necessary to identify QTL for resistance to seed rot. The interaction between P. irregulare or P. sylvaticum with either Fusarium oxysporum or F. graminearum on soybean, was evaluated in a cup assay under controlled environmental conditions. Each interaction was evaluated in a separate experiment that was repeated three times. Controls included a non-inoculated control, and a Pythium-only and Fusarium-only treatment, the species of which corresponded to the interaction being tested. Disease development parameters, disease severity (DS), wet root weight (WRW), and emergence (EME) were assessed at five sampling times, 2, 4, 6, 8, and ...

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Metabolic Diversity and Novelties in the Oomycetes

Judelson

2017

Annu. Rev. Microbiol.

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The eukaryotic microbes called oomycetes include many important saprophytes and pathogens, with the latter exhibiting necrotrophy, biotrophy, or obligate biotrophy. Understanding oomycete metabolism is fundamental to understanding these lifestyles. Genome mining and biochemical studies have shown that oomycetes, which belong to the kingdom Stramenopila, secrete suites of carbohydrate- and protein-degrading enzymes adapted to their environmental niches and produce unusual lipids and energy storage compounds. Despite having limited secondary metabolism, many oomycetes make chemicals for communicating within their species or with their hosts. Horizontal and endosymbiotic gene transfer events have diversified oomycete metabolism, resulting in biochemical pathways that often depart from standard textbook descriptions by amalgamating enzymes from multiple sources. Gene fusions and duplications have further shaped the composition and expression of the enzymes. Current research is helping us learn how oomycetes interact with host and environment, understand eukaryotic diversity and evolution, and identify targets for drugs and crop protection chemicals.

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Transcriptome and secretome of two Pythium species during infection and saprophytic growth

Cited by 5 publications

References 113 publications

Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Soybean-Pythium pathosystem: Search for host resistance and interaction with Fusarium species

Metabolic Diversity and Novelties in the Oomycetes

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