Transcriptome and targeted metabolome analysis provide insights into bile acids' new roles and mechanisms on fat deposition and meat quality in lamb

Zhang, Boyan; Sun, Zhiqiang; Zhu, Yan; Li, Wei; Liu, Chunhai; Li, Haihai; Luo, Hui; Wang, Bing

doi:10.1016/j.foodres.2022.111941

Cited by 14 publications

(10 citation statements)

References 70 publications

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“…Glycocholic acid is a sterol lipid, and is enriched in the bile acid biosynthesis pathway, acting as a primary bile acid [42]. Bile acids, primarily synthesized in the liver from cholesterol, can solubilize dietary fat and promote the absorption of fat and glucose homeostasis when they are secreted into the intestine [43]. Yang et al [44] reported that bile acid promoted the hydrolysis of fats via lipases to release more FA, thus increasing energy expenditure in animals.…”

Section: Discussionmentioning

confidence: 99%

The Characterization of Subcutaneous Adipose Tissue in Sunit Sheep at Different Growth Stages: A Comprehensive Analysis of the Morphology, Fatty Acid Profile, and Metabolite Profile

Han,

He,

Yun

et al. 2024

Foods

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Adipose tissue is a crucial economically significant trait that significantly influences the meat quality and growth performance of domestic animals. To reveal the changes in adipose tissue metabolism during the growth of naturally grazing sheep, we evaluated the thickness, adipocyte morphology, fatty acid profile, and metabolite profile of subcutaneous adipose tissue (SAT) from naturally grazing Sunit sheep at 6, 18, and 30 months of age (referred to as Mth-6, Mth-18, and Mth-30, respectively). The fat thickness and adipocyte number were significantly increased with the growth of the sheep (p < 0.05), and the increase of which from Mth-18 to Mth-30 was less than that from Mth-6 to Mth-18. Additionally, the alpha-linolenic acid metabolism was enhanced and fatty acid (FA) elongation increased with growth. The metabolomic analysis revealed 76 differentially expressed metabolites (DEMs) in the SAT in different growth stages. Interestingly, we observed elongation of FAs in lipids correlated with sheep growth. Furthermore, the expression of acylcarnitines was downregulated, and fatty acid amides, aspartic acid, acetic acid and phosphocholine were upregulated in Mth-18 and Mth-30 compared to Mth-6. Altogether, the study found that the difference in SAT in Mth-6 was great compared to Mth-18 and Mth-30. An increase in fat deposition via adipocyte proliferation with the growth of the sheep in naturally grazing. The DEMs of acylcarnitines, fatty acid amides, aspartic acid, acetic acid, and phosphocholine emerged as potential key regulators of adipose tissue metabolism. These findings illustrate the variation in and metabolic mechanism of sheep adipose tissue development under natural grazing, thus providing valuable insights into improving the edible quality of sheep meat and developing the mutton sheep industry.

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Section: Discussionmentioning

confidence: 99%

The Characterization of Subcutaneous Adipose Tissue in Sunit Sheep at Different Growth Stages: A Comprehensive Analysis of the Morphology, Fatty Acid Profile, and Metabolite Profile

Han,

He,

Yun

et al. 2024

Foods

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“…The functional role and mechanism of bile acids in lamb fat deposition were scrutinized using RNA-seq and targeted metabolomics. This analysis unveiled differential genes associated with ferritin and fatty acid biosynthesis, including HSPA8, HIF1A, HEXB, ACSL6, and MAP1LC3B, linked to tail lipid weight and proportions, potentially serving as marker genes for tail lipid regulation [79]. Additionally, an investigation utilizing RNA-seq and metabolome association analysis identified key genes governing muscle flavor precursors in sheep.…”

Section: Meat Traitsmentioning

confidence: 93%

Pan-Omics in Sheep: Unveiling Genetic Landscapes

Li,

Lu,

Gao

et al. 2024

Animals

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Multi-omics-integrated analysis, known as panomics, represents an advanced methodology that harnesses various high-throughput technologies encompassing genomics, epigenomics, transcriptomics, proteomics, and metabolomics. Sheep, playing a pivotal role in agricultural sectors due to their substantial economic importance, have witnessed remarkable advancements in genetic breeding through the amalgamation of multiomics analyses, particularly with the evolution of high-throughput technologies. This integrative approach has established a robust theoretical foundation, enabling a deeper understanding of sheep genetics and fostering improvements in breeding strategies. The comprehensive insights obtained through this approach shed light on diverse facets of sheep development, including growth, reproduction, disease resistance, and the quality of livestock products. This review primarily focuses on the application of principal omics analysis technologies in sheep, emphasizing correlation studies between multiomics data and specific traits such as meat quality, wool characteristics, and reproductive features. Additionally, this paper anticipates forthcoming trends and potential developments in this field.

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“…In addition, the expression of MYOD1 can inhibit adipogenic differentiation of skeletal muscle satellite cells. After feeding sheep with probiotics, MYOD1 expression is silenced, leading to increased intramuscular fat content ( Zhang et al, 2022a ; Zhao et al., 2022a ). It has been also reported that genes related to muscle fiber compositions are differentially expressed, which can affect glycolysis and meat color ( Su et al, 2019 ).…”

Section: Transcriptomics Techniques and Application For Meat Qualitymentioning

confidence: 99%

Multi-Omics Approaches to Improve Meat Quality and Taste Characteristics

Hwang,

Lee,

Lim

et al. 2023

Food Sci Anim Resour

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With rapid advances in meat science in recent decades, changes in meat quality during the pre-slaughter phase of muscle growth and the post-slaughter process from muscle to meat have been investigated. Commonly used techniques have evolved from early physicochemical indicators such as meat color, tenderness, water holding capacity, flavor, and pH to various omic tools such as genomics, transcriptomics, proteomics, and metabolomics to explore fundamental molecular mechanisms and screen biomarkers related to meat quality and taste characteristics. This review highlights the application of omics and integrated multi-omics in meat quality and taste characteristics studies. It also discusses challenges and future perspectives of multi-omics technology to improve meat quality and taste. Consequently, multi-omics techniques can elucidate the molecular mechanisms responsible for changes of meat quality at transcriptome, proteome, and metabolome levels. In addition, the application of multi-omics technology has great potential for exploring and identifying biomarkers for meat quality and quality control that can make it easier to optimize production processes in the meat industry.

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Transcriptome and targeted metabolome analysis provide insights into bile acids' new roles and mechanisms on fat deposition and meat quality in lamb

Cited by 14 publications

References 70 publications

The Characterization of Subcutaneous Adipose Tissue in Sunit Sheep at Different Growth Stages: A Comprehensive Analysis of the Morphology, Fatty Acid Profile, and Metabolite Profile

The Characterization of Subcutaneous Adipose Tissue in Sunit Sheep at Different Growth Stages: A Comprehensive Analysis of the Morphology, Fatty Acid Profile, and Metabolite Profile

Pan-Omics in Sheep: Unveiling Genetic Landscapes

Multi-Omics Approaches to Improve Meat Quality and Taste Characteristics

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