Transcriptome-Based Comparative Analysis of Transcription Factors in Response to NaCl, NaOH, and Na2CO3 Stresses in Roots of Autotetraploid Rice (Oryza sativa L.)

Abstract

Soil salinity is a severe constraint on crop productivity that triggers plant salt stress response pathways. The salt stress responses are controlled by transcriptional regulatory networks that enhance plant stress tolerance by manipulating the expression of transcription factor (TFs)-encoding genes. Identifying TFs associated with salt tolerance contributes to salt-tolerant crop breeding. In this study, a comparative transcriptome analysis was performed to investigate the global gene expression of entire TFs in diploid and autotetraploid rice with different salt tolerance levels, considering NaCl stress, NaOH stress, and Na2CO3 stress. A total of 54, 54 and 55 TF families were co-expressed in diploid and tetraploid roots under three stresses, respectively. Furthermore, we investigated differentially expressed TFs (DE-TFs) based on different comparisons, and the statistical analysis indicated that the DE-TFs derived from the three types of stress were as follows: Na2CO3 (53 TF families, 1356 DE-TFs) > NaCl (19 TF families, 214 DE-TFs) > NaOH (18 TF families, 152 DE-TFs). These results imply that Na2CO3 stress induced a more obvious biological responses in the roots than the other two stresses. GO and KEGG pathway enrichment analysis of DE-TFs revealed the importance of plant hormone signal transduction and MAPK signaling pathways that may contribute to the saline–alkaline tolerance of tetraploid rice. This study provides gene resources that are valuable for exploring the regulatory mechanism of TFs involved in the saline–alkaline tolerance of polyploid rice.

Evaluating the Differential Response of Transcription Factors in Diploid versus Autotetraploid Rice Leaves Subjected to Diverse Saline–Alkali Stresses

“…Meanwhile, our study confirms the positive regulatory role of OsNAC10 in response to salt and alkali stress, especially under NaCl and Na 2 CO 3 stress. We also investigated the DETF genes under saline–alkali stress in the root of diploid and autotetraploid rice in a previous report [ 38 ]; there were many DETF genes related to the phytohormone response, however, due to the tissue specificity of gene expression, we found that there were significant differences between the roots and leaves of rice under saline–alkali stress. It was therefore necessary to study the DETF genes that respond to saline–alkali stress in the leaves of rice.…”

Bio-functionalized nickel-silica nanoparticles suppress bacterial leaf blight disease in rice (Oryza sativa L.)

“…As NPs have positive or low negative charges, they are electrostatically attracted and adhered to the negatively charged cell membrane of bacteria ( Zein El-Abdeen and Farroh, 2019 ). Subsequently, it caused irregular pit formations on the cell wall of the pathogenic bacteria that facilitate the entry of NPs into periplasmic space and inside bacterial cells ( Ninganagouda et al., 2014 ; Wang et al., 2023 ). The high efficacy of Ni-SiO 2 NP composite against Xoo could be due to the size of the nickel NPs that have been reduced by silicon to range approximately from 10 to 30 nm, which allows nickel NPs to intensively enter the bacterial cell, resulting in ion accumulation that contributes to membrane porosity damaging the cytoplasm and cell structures.…”

“…Rice ( Oryza sativa L.) is the most consumed cereal crop worldwide. Food and Agriculture Organization considers rice as an important crop for food security in the world ( Wang et al., 2023 ). One of the most serious diseases infecting rice plants is bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv.…”