Transcriptome-derived investigation of biosynthesis of quinolizidine alkaloids in narrow-leafed lupin (Lupinus angustifolius L.) highlights candidate genes linked to iucundus locus

Kroc, Magdalena; Koczyk, Grzegorz; Kamel, Katarzyna; Czepiel, Katarzyna; Fedorowicz-Strońska, Olga; Krajewski, Paweł; Kosińska, Joanna; Podkowiński, Jan; Wilczura, Paulina; Świecicki, W.

doi:10.1038/s41598-018-37701-5

Cited by 35 publications

(63 citation statements)

References 79 publications

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“…Specifically, an allele marker, IucLi, has been identified for the iucundus locus, and could be used for MAS in wild × domesticated crosses in lupin breeding programs (Li et al, 2011). Recently, 12 candidate genes for the alkaloid locus iucundus and the major QTLs associated with total QA contents were identified using a transcriptomic approach (Kroc et al, 2019b). The most promising candidate, RAP2-7, encodes an ethylene-responsive transcription factor (ERF) that co-segregated with the iucundus locus and is likely to be involved in the regulation of QA biosynthesis in L. angustifolius (Kroc et al, 2019a).…”

Section: Defense-related Traitsmentioning

confidence: 99%

“…The most promising candidate, RAP2-7, encodes an ethylene-responsive transcription factor (ERF) that co-segregated with the iucundus locus and is likely to be involved in the regulation of QA biosynthesis in L. angustifolius (Kroc et al, 2019a). Other candidate genes include a 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS) involved in Lys biosynthesis as well as genes involved in plant secondary metabolism (Kroc et al, 2019b).…”

Section: Defense-related Traitsmentioning

confidence: 99%

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The Effects of Domestication on Secondary Metabolite Composition in Legumes

Contador

et al. 2020

Front. Genet.

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Legumes are rich in secondary metabolites, such as polyphenols, alkaloids, and saponins, which are important defense compounds to protect the plant against herbivores and pathogens, and act as signaling molecules between the plant and its biotic environment. Legume-sourced secondary metabolites are well known for their potential benefits to human health as pharmaceuticals and nutraceuticals. During domestication, the color, smell, and taste of crop plants have been the focus of artificial selection by breeders. Since these agronomic traits are regulated by secondary metabolites, the basis behind the genomic evolution was the selection of the secondary metabolite composition. In this review, we will discuss the classification, occurrence, and health benefits of secondary metabolites in legumes. The differences in their profiles between wild legumes and their cultivated counterparts will be investigated to trace the possible effects of domestication on secondary metabolite compositions, and the advantages and drawbacks of such modifications. The changes in secondary metabolite contents will also be discussed at the genetic level to examine the genes responsible for determining the secondary metabolite composition that might have been lost due to domestication. Understanding these genes would enable breeding programs and metabolic engineering to produce legume varieties with favorable secondary metabolite profiles for facilitating adaptations to a changing climate, promoting beneficial interactions with biotic factors, and enhancing health-beneficial secondary metabolite contents for human consumption.

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Section: Defense-related Traitsmentioning

confidence: 99%

Section: Defense-related Traitsmentioning

confidence: 99%

The Effects of Domestication on Secondary Metabolite Composition in Legumes

Contador

et al. 2020

Front. Genet.

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“…The alkaloid biosynthetic pathway of the lupins has not yet been fully characterized [17] ( Figure 1). The best-studied pathway at the genetic level is QA biosynthesis in narrow-leafed lupin and other crop species [24][25][26][27][28][29][30][31][32][33]. For further lupin breeding, which includes the domestication of new species, it is important to unravel alkaloid biosynthesis and to valorize lupin genetic resources [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination

Świecicki

Czepiel

Wilczura

et al. 2019

Plants

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The total contents and qualitative compositions of alkaloids in seeds of 10 Old World lupin species (73 accessions) were surveyed using gas chromatography. The obtained results, combined with those for three lupin crops, Lupinus angustifolius, Lupinus albus, and Lupinus luteus, provide the most complete and up-to-date overview of alkaloid profiles of 13 lupin species originating from the Mediterranean Basin. The qualitative alkaloid compositions served as useful supplementary tools of species discrimination. On the basis of the most abundant major alkaloids, lupanine, lupinine, and multiflorine, the Old World lupin species were divided into four groups. Those containing lupanine (L. angustifolius, L. albus, and Lupinus mariae-josephi), containing lupinine (Lupinus luteus, Lupinus hispanicus, and Lupinus × hispanicoluteus), containing lupinine and multiflorine (Lupinus atlanticus, Lupinus palaestinus, Lupinus anatolicus, Lupinus digitatus, Lupinus pilosus, and Lupinus cosentinii), and containing multiflorine (Lupinus micranthus). Within a given group, certain species can be, in most cases, further distinguished by the presence of other major alkaloids. The discrimination of species based on the total alkaloid content was found to be less reliable because of the significant intra-species variations, as well as the influences of environmental factors on the seed alkaloid content.

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“…Transcriptome sequencing (RNA-seq) and analysis of differentially expressed genes in a sample containing bitter and sweet narrow-leaved lupine accessions helped to detect 13 genes presumably involved in the synthesis of quinolizidine alkaloids (Kamel et al, 2016). The identified alkaloid biosynthesis genes were mapped, but only one transcriptomic factor from the RAP2 family of factors regulating secondary metabolism was closely linked with the iuc gene (Kroc et al, 2019). Investigating the mapping populations with the technique of massive analysis for cDNA ends (MACE) confirmed the idea that the ETHYLENE-RESPONSIVE TRANSCRIPTION RAC2-7 gene factor could control the low-alkaloid phenotype in narrow-leaved lupine (Plewiński et al, 2019).…”

Section: Genetic and Genomic Resources Of Narrow-leaved Lupinementioning

confidence: 99%

Alkaloids of narrow-leaved lupine as a factor determining alternative ways of the crop’s utilization and breeding

et al. 2020

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Narrow-leaved lupine (Lupinus angustifolius L.), a valuable leguminous crop adapted to a wide range of climatic conditions, has a very short history of domestication. For many centuries it was used mainly as a green manure, since the success and prospects of the multi-purpose use of the species depend on its breeding improvement, in particular, on a particular concentration of alkaloids in seeds and green mass. The first varieties of scientific breeding were created only in the 1930s after the appearance of low-alkaloid mutants. Despite wide prospects for use in various areas of the national economy, unstable productivity and susceptibility to diseases hinder the production of this crop. Obviously, breeders deal only with a small part of the gene pool of the species and limited genetic resources, using mainly low-alkaloid (sweet) genotypes to create new varieties. The genetic potential of the species can be used more efficiently. At the same time, it is rational to create highly alkaloid (bitter) varieties for green manure, while food and feed varieties should not lose their adaptive potential, in particular, resistance to pathogens, due to the elimination of alkaloids. In this regard, it seems to be a productive idea to create ‘bitter/sweet’ varieties combining a high content of alkaloids in the vegetative organs and low in seeds, which can be achieved by regulating the synthesis/transport of alkaloids in the plant. The paper discusses the current state of use of the species as a green manure, fodder, food plant. Information is given on the quantity and qualitative composition of narrow-leaved lupine alkaloids, their applied value, in particular, fungicidal, antibacterial, insecticidal, the use of lupine alkaloids as active principles of drugs. Along with promising breeding considerations, the possibility of using technologies for processing raw high-alkaloid materials with the accompanying extraction of valuable ingredients for pharmaceuticals is discussed. Information is briefly presented about the genomic resources of the species and the prospects for their use in marker-assistant selection and genome editing.

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Transcriptome-derived investigation of biosynthesis of quinolizidine alkaloids in narrow-leafed lupin (Lupinus angustifolius L.) highlights candidate genes linked to iucundus locus

Cited by 35 publications

References 79 publications

The Effects of Domestication on Secondary Metabolite Composition in Legumes

The Effects of Domestication on Secondary Metabolite Composition in Legumes

Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination

Alkaloids of narrow-leaved lupine as a factor determining alternative ways of the crop’s utilization and breeding

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