Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties

Yan, Huishu; Shi, Huawei; Hu, Chengmei; Luo, Mingzhao; Xu, Chengjie; Wang, Shuguang; Li, Ning; Tang, Wensi; Zhou, Yongbin; Wang, Chunxiao; Xu, Zhao-Shi; Ma, You-Zhi; Sun, Daizhen; Chen, Ming

doi:10.3390/ijms222212278

Cited by 14 publications

(15 citation statements)

References 44 publications

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“…RNA transcript profiling can rapidly and effectively provide information for genome-wide transcript characterization, differential gene expression analysis, variant detection, and gene-specific expression. This technology is used to analyze the transcriptome in response to different biotic or abiotic stresses, including low-nitrogen stress [ 48 ], high-light stress [ 49 ], drought response to different soybean cultivars [ 50 ], salt stress [ 51 , 52 ], Cd stress [ 53 ], and sweet orange response to Citrus tristeza virus [ 54 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Tryptophan-Induced Resistance against Potato Common Scab

Zhao

Liu

Cao

et al. 2022

IJMS

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Potato common scab (CS) is a worldwide soil-borne disease that severely reduces tuber quality and market value. We observed that foliar application of tryptophan (Trp) could induce resistance against CS. However, the mechanism of Trp as an inducer to trigger host immune responses is still unclear. To facilitate dissecting the molecular mechanisms, the transcriptome of foliar application of Trp and water (control, C) was compared under Streptomyces scabies (S) inoculation and uninoculation. Results showed that 4867 differentially expressed genes (DEGs) were identified under S. scabies uninoculation (C-vs-Trp) and 2069 DEGs were identified under S. scabies inoculation (S-vs-S+Trp). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that Trp induced resistance related to the metabolic process, response to stimulus, and biological regulation. As phytohormone metabolic pathways related to inducing resistance, the expression patterns of candidate genes involved in salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) pathways were analyzed using qRT-PCR. Their expression patterns showed that the systemic acquired resistance (SAR) and induced systemic resistance (ISR) pathways could be co-induced by Trp under S. scabies uninoculation. However, the SAR pathway was induced by Trp under S. scabies inoculation. This study will provide insights into Trp-induced resistance mechanisms of potato for controlling CS, and extend the application methods of Trp as a plant resistance inducer in a way that is cheap, safe, and environmentally friendly.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Tryptophan-Induced Resistance against Potato Common Scab

Zhao

Liu

Cao

et al. 2022

IJMS

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“…Under LN conditions, plants undergo significant transcriptional reprogramming and developmental alteration [ 56 , 57 ]. The reshaping of plant architecture emerges as a common adaptive strategy, representing a long-term response to LN environments.…”

Section: Epigenetic Regulation Facilitates Ln Adaptationmentioning

confidence: 99%

Epigenetic Regulation of Nitrogen Signaling and Adaptation in Plants

Zhang

Xiao

2023

Plants

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Nitrogen (N) is a crucial nutrient that plays a significant role in enhancing crop yield. Its availability, including both supply and deficiency, serves as a crucial signal for plant development. However, excessive N use in agriculture leads to environmental and economic issues. Enhancing nitrogen use efficiency (NUE) is, therefore, essential to minimize negative impacts. Prior studies have investigated the genetic factors involved in N responses and the process of low-nitrogen (LN) adaptation. In this review, we discuss recent advances in understanding how epigenetic modifications, including DNA methylation, histone modification, and small RNA, participate in the regulation of N response and LN adaptation. We highlight the importance of decoding the epigenome at various levels to accelerate the functional study of how plants respond to N availability. Understanding the epigenetic control of N signaling and adaptation can lead to new strategies to improve NUE and enhance crop productivity sustainably.

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“…With the development of biotechnology, transcriptomic data have become an important tool for understanding the potential mechanisms of gene expression [38,39]. Over the past decades, a large amount of N-related transcriptomic data has been analyzed in crops to understand the N response mechanisms [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58]. In rice, the expression of genes related to energy metabolism, amino acid metabolism, and carbohydrate metabolism was reduced under LN stress [40].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling Reveals the Gene Network Responding to Low Nitrogen Stress in Wheat

Wang,

Li,

Zhu

et al. 2024

Plants

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As one of the essential nutrients for plants, nitrogen (N) has a major impact on the yield and quality of wheat worldwide. Due to chemical fertilizer pollution, it has become increasingly important to improve crop yield by increasing N use efficiency (NUE). Therefore, understanding the response mechanisms to low N (LN) stress is essential for the regulation of NUE in wheat. In this study, LN stress significantly accelerated wheat root growth, but inhibited shoot growth. Further transcriptome analysis showed that 8468 differentially expressed genes (DEGs) responded to LN stress. The roots and shoots displayed opposite response patterns, of which the majority of DEGs in roots were up-regulated (66.15%; 2955/4467), but the majority of DEGs in shoots were down-regulated (71.62%; 3274/4565). GO and KEGG analyses showed that nitrate reductase activity, nitrate assimilation, and N metabolism were significantly enriched in both the roots and shoots. Transcription factor (TF) and protein kinase analysis showed that genes such as MYB-related (38/38 genes) may function in a tissue-specific manner to respond to LN stress. Moreover, 20 out of 107 N signaling homologous genes were differentially expressed in wheat. A total of 47 transcriptome datasets were used for weighted gene co-expression network analysis (17,840 genes), and five TFs were identified as the potential hub regulatory genes involved in the response to LN stress in wheat. Our findings provide insight into the functional mechanisms in response to LN stress and five candidate regulatory genes in wheat. These results will provide a basis for further research on promoting NUE in wheat.

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Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties

Cited by 14 publications

References 44 publications

Transcriptome Analysis of Tryptophan-Induced Resistance against Potato Common Scab

Transcriptome Analysis of Tryptophan-Induced Resistance against Potato Common Scab

Epigenetic Regulation of Nitrogen Signaling and Adaptation in Plants

Transcriptome Profiling Reveals the Gene Network Responding to Low Nitrogen Stress in Wheat

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