Transcriptome dynamics of Camellia sinensis in response to continuous salinity and drought stress

Zhang, Qing; Cai, Muchen; Yu, Xiaomin; Wang, Lishan; Guo, Chunfang; Ming, Ray; Zhang, Jisen

doi:10.1007/s11295-017-1161-9

Cited by 77 publications

(66 citation statements)

References 75 publications

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“…To further depict the landscape of cold tolerance in tea plant, we also generated a total of 161 Gb RNA‐seq data from five stages of tea plant during cold acclimation, including nonacclimated at 25~20 °C (CK), fully acclimated at 10 °C for 6 h (CA1‐6 h) and 10~4 °C for 7 days (CA1‐7d), cold response at 4~0 °C for 7 days (CA2‐7d) and recovering under 25~20 °C for 7 days (DA‐7d). (ii) Drought tolerance: a total of 108.82 million RNA‐seq reads were collected from young leaves of tea plant subjected to continuous drought stress, including four stages: 25% polyethylene glycol (PEG) treatment for 0, 24, 48 and 72 h (Zhang et al ., ). The total length of the sequencing data is 5.5 Gb.…”

Section: Data Contentmentioning

confidence: 97%

“…The total length of the sequencing data is 5.5 Gb. (iii) Salinity stress: a total of 149.48 million RNA‐seq reads (7.55 Gb) were collected from leaves of tea plant under salt stress (Zhang et al ., ). The 200 m m NaCl was used to simulate salt‐stress conditions for tea plant with 0, 24, 48 and 72 h. (iv) methyl jasmonate (MeJA) response: RNA‐seq data from leaves of tea plant in response to MeJA were collected from Shi et al .…”

Section: Data Contentmentioning

confidence: 97%

See 1 more Smart Citation

Tea Plant Information Archive: a comprehensive genomics and bioinformatics platform for tea plant

Xia

Tong

et al. 2019

Plant Biotechnology Journal

237

181

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Summary Tea is the world's widely consumed nonalcohol beverage with essential economic and health benefits. Confronted with the increasing large‐scale omics‐data set particularly the genome sequence released in tea plant, the construction of a comprehensive knowledgebase is urgently needed to facilitate the utilization of these data sets towards molecular breeding. We hereby present the first integrative and specially designed web‐accessible database, Tea Plant Information Archive (TPIA; http://tpia.teaplant.org). The current release of TPIA employs the comprehensively annotated tea plant genome as framework and incorporates with abundant well‐organized transcriptomes, gene expressions (across species, tissues and stresses), orthologs and characteristic metabolites determining tea quality. It also hosts massive transcription factors, polymorphic simple sequence repeats, single nucleotide polymorphisms, correlations, manually curated functional genes and globally collected germplasm information. A variety of versatile analytic tools (e.g. JBrowse, blast, enrichment analysis, etc.) are established helping users to perform further comparative, evolutionary and functional analysis. We show a case application of TPIA that provides novel and interesting insights into the phytochemical content variation of section Thea of genus Camellia under a well‐resolved phylogenetic framework. The constructed knowledgebase of tea plant will serve as a central gateway for global tea community to better understand the tea plant biology that largely benefits the whole tea industry.

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Section: Data Contentmentioning

confidence: 97%

Section: Data Contentmentioning

confidence: 97%

Tea Plant Information Archive: a comprehensive genomics and bioinformatics platform for tea plant

Xia

Tong

et al. 2019

Plant Biotechnology Journal

237

181

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“…An average of 11.8 Gb of clean RNA-seq data were generated electronically for each of the eight tissue types from published data 37 . For the treatments of salinity (200 mM NaCl) and drought (induced by 25% PEG, Polyethylene glycol), equal amounts of total RNA extracted from tea shoot tips collected at each time interval (0 h, 24 h, 48 h, 72 h) after stress or non-stressed treatment were pooled together for transcriptome analysis 53 . To study the effects of cold acclimation (10 °C ~ 4 °C for 7 days) and non-acclimation (25~20 °C, CK) on tea plant TPS gene expression, data were retrieved from TPIA database 37 .…”

Section: Gene Expression Analysismentioning

confidence: 99%

Analysis of Terpene Synthase Family Genes in Camellia sinensis with an Emphasis on Abiotic Stress Conditions

Zhou

Shamala

et al. 2020

Sci Rep

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For a better understanding terpenoid volatile production in Camellia sinensis, global terpenoid synthase gene (TPS) transcription analysis was conducted based on transcriptomic data combined with terpenoid metabolic profiling under different abiotic stress conditions. Totally 80 TPS-like genes were identified. twenty-three CsTPS genes possessed a complete coding sequence and most likely were functional. The remaining 57 in the currently available database lack essential gene structure or full-length transcripts. Distinct tempo-spatial expression patterns of CsTPS genes were found in tea plants. 17 genes were substantially expressed in all the tested organs with a few exceptions. The other 17 were predominantly expressed in leaves whereas additional eight were primarily expressed in flowers. Under the treatments of cold acclimation, salt and polyethylene glycol, CsTPS67,-69 and-71 were all suppressed and the inhibited expression of many others were found in multiple stress treatments. However, methyl jasmonate resulted in the enhanced expression of the majority of CsTPS genes. These transcription data were largely validated using qPCR. Moreover, volatile terpenoid profiling with leaves, flowers and stress-treated plants revealed a general association between the abundances of mono-and sesqui-terpenoids and some CsTPS genes. These results provide vital information for future studies on CsTPS regulation of terpenoid biosynthesis. Plant terpenoids (isoprene-C5, monoterpenes-C10, sesquiterpenes-C15, diterpenes-C20, and polyterpenoids-C5xn) possess diverse functions in plant growth and development 1-7. They play significant ecological roles in the interactions between plants and stress conditions. Generally, terpenoid molecules smaller than diterpenoids are volatile and well known for their airborne signaling function, particularly against herbivore attack 8,9. High volatility of monoterpenes and sesquiterpenes enhances the flavor and aroma of crop products 10 such as tea, which is a popular beverage well known for its fragrance and aroma 11. Tea volatile terpenoids not only are defense components against insects 12 or high solar radiation 13 , but are also essential odorants of tea products with a direct influence on flavor and quality 14-16. Aroma from volatile terpenoids is one of the main sensory properties affecting tea flavor quality 17. For instance, monoterpene alcohols such as linalool and geraniol, two of the most abundant and odor active terpenoids in tea 15 , impart pleasant floral scent to green tea and black tea 17. Terpene synthases possess a characteristic catalytic function that generates multiple terpenoid products with one substrate 18 , thus collectively contributing to numerous and different structures of plant terpenoids in addition to other modifying enzymes such as uridine diphosphate (UDP)-glucosyl transferases 19,20 and P450s 21. TPSs are responsible for converting the precursors of geranyl diphosphate (GPP), isoprenyl diphosphate (IPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphat...

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“…Salinity and drought tolerance is another focus of plant breeding and cultivation and has an important influence on plant growth [ 54 ]. To understand the gene expression profiles, the RNA-seq experiments under drought and salt stress treatments of C. sinensis were conducted, and 3936 and 3715 differentially expressed genes (DEGs) were identified upon drought and salt stress, respectively [ 20 ]. Further comparative analyses of DEGs indicated that these two stresses had some common molecular effects, which indicates that shared pathways were involved [ 20 ].…”

Section: Genetics and Genomic Resources In Genus Camellimentioning

confidence: 99%

“…To understand the gene expression profiles, the RNA-seq experiments under drought and salt stress treatments of C. sinensis were conducted, and 3936 and 3715 differentially expressed genes (DEGs) were identified upon drought and salt stress, respectively [ 20 ]. Further comparative analyses of DEGs indicated that these two stresses had some common molecular effects, which indicates that shared pathways were involved [ 20 ]. The seed germination process of Camellia was also found to be sensitive to drought stress.…”

Section: Genetics and Genomic Resources In Genus Camellimentioning

confidence: 99%

Unraveling the Roles of Regulatory Genes during Domestication of Cultivated Camellia: Evidence and Insights from Comparative and Evolutionary Genomics

Yan

Lin

Lyu

et al. 2018

Genes

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With the increasing power of DNA sequencing, the genomics-based approach is becoming a promising resolution to dissect the molecular mechanism of domestication of complex traits in trees. Genus Camellia possesses rich resources with a substantial value for producing beverage, ornaments, edible oil and more. Currently, a vast number of genetic and genomic research studies in Camellia plants have emerged and provided an unprecedented opportunity to expedite the molecular breeding program. In this paper, we summarize the recent advances of gene expression and genomic resources in Camellia species and focus on identifying genes related to key economic traits such as flower and fruit development and stress tolerances. We investigate the genetic alterations and genomic impacts under different selection programs in closely related species. We discuss future directions of integrating large-scale population and quantitative genetics and multiple omics to identify key candidates to accelerate the breeding process. We propose that future work of exploiting the genomic data can provide insights related to the targets of domestication during breeding and the evolution of natural trait adaptations in genus Camellia.

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Transcriptome dynamics of Camellia sinensis in response to continuous salinity and drought stress

Cited by 77 publications

References 75 publications

Tea Plant Information Archive: a comprehensive genomics and bioinformatics platform for tea plant

Tea Plant Information Archive: a comprehensive genomics and bioinformatics platform for tea plant

Analysis of Terpene Synthase Family Genes in Camellia sinensis with an Emphasis on Abiotic Stress Conditions

Unraveling the Roles of Regulatory Genes during Domestication of Cultivated Camellia: Evidence and Insights from Comparative and Evolutionary Genomics

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