Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation

Geng-Hua, Chen; Yin, Yunqian; Lin, Zetong; Wen, Huaqiang; Chen, Jiahui; Luo, Wen

doi:10.1016/j.bbrc.2021.04.086

Cited by 6 publications

(6 citation statements)

References 42 publications

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“…Cellular and molecular events are required for the coordination of chicken skeletal muscle growth and development ( Chen et al, 2021a ). For example, CDK1 has an important role in myoblast proliferation, the cell cycle, muscle regeneration, and myofiber hypertrophy in yaks and mice ( Ji et al, 2020 ; Kobayashi et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Meat production traits are the most important economic traits in broiler production, and one of the important goals of the poultry industry is to increase meat production ( Kui et al, 2022 ). In chickens, skeletal muscle accounts for approximately half of the total body weight and plays important roles in both metabolism and growth and development ( Bottinelli and Reggiani, 2000 ), but there are still many unexplored gene expression patterns ( Llano-Diez et al, 2019 ; Camps et al, 2020 ; Bamba et al, 2022 ; Han et al, 2022 ), including myoblast proliferation, differentiation, and regulation of transcription factors ( Chen et al, 2021a ). Due to the developmental anatomy of chickens is similar to that of mammals, it is an ideal model animals for studying skeletal muscle development ( Berti et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration

Chen,

Zhang,

Niu

et al. 2024

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration

Chen,

Zhang,

Niu

et al. 2024

Poultry Science

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“…Using the High-Capacity Reverse Transcription Kit with random primers (Applied Biosystems, Waltham, MA, USA) according to manufacturer’s instructions, complementary deoxyribonuclease (cDNA) synthesis was performed for 500 ng (cTEC supernatants) or 2000 ng (tracheas) of RNA per sample. qPCR targeting the IBV nucleoprotein gene (N) was performed for quantification of IBV viral genome load in cTEC supernatants and trachea and for host mRNA gene expression in the tracheal tissues, using gene-specific primers ( Table S1 , [ 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 ]) at a final concentration of 5 nM (Sigma-Aldrich, Saint-Louis, MO, USA) and PowerUp SYBR Green Master Mix (Applied Biosystems, Burlington, ON, Canada) in a 20 µL reaction according to the manufacturer’s instructions. Furthermore, a 10-fold dilution series of the IBV-N gene plasmid was used to generate the standard curve, as previously described [ 68 ].…”

Section: Methodsmentioning

confidence: 99%

Host Immune Response Modulation in Avian Coronavirus Infection: Tracheal Transcriptome Profiling In Vitro and In Vivo

O’Dowd,

Isham,

Vatandour

et al. 2024

Viruses

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Infectious bronchitis virus (IBV) is a highly contagious Gammacoronavirus causing moderate to severe respiratory infection in chickens. Understanding the initial antiviral response in the respiratory mucosa is crucial for controlling viral spread. We aimed to characterize the impact of IBV Delmarva (DMV)/1639 and IBV Massachusetts (Mass) 41 at the primary site of infection, namely, in chicken tracheal epithelial cells (cTECs) in vitro and the trachea in vivo. We hypothesized that some elements of the induced antiviral responses are distinct in both infection models. We inoculated cTECs and infected young specific pathogen-free (SPF) chickens with IBV DMV/1639 or IBV Mass41, along with mock-inoculated controls, and studied the transcriptome using RNA-sequencing (RNA-seq) at 3 and 18 h post-infection (hpi) for cTECs and at 4 and 11 days post-infection (dpi) in the trachea. We showed that IBV DMV/1639 and IBV Mass41 replicate in cTECs in vitro and the trachea in vivo, inducing host mRNA expression profiles that are strain- and time-dependent. We demonstrated the different gene expression patterns between in vitro and in vivo tracheal IBV infection. Ultimately, characterizing host–pathogen interactions with various IBV strains reveals potential mechanisms for inducing and modulating the immune response during IBV infection in the chicken trachea.

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“…However, the function of this gene is totally obscure. According to a recently pub-lished report, KLHL30 is essential regulator for myoblast differentiation [116]. Considering that this gene is strongly upregulated in different cancer cell lines in response to various stress factors, one can guess that its biological function exceeds myoblast differentiation.…”

Section: Atypical Targets Of P53mentioning

confidence: 99%

The p53 protein – not only the guardian of the genome

Rusin

2024

Postepy Biochem

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The p53 tumor suppressor protein is best known as an activator of cell cycle arrest and apoptosis. Only a fraction of p53-activated genes encode proteins affecting cellular replication and various forms of cell death (apoptosis, ferroptosis, autophagy). The p53-regulated genes can be divided into so-called the core transcriptional program, which comprises genes activated in most cell types by most activators, and into the group of genes activated in in cell- or stress-specific manner. Activation of p53 occurs via the extensive set of posttranslational modifications, which adjust its stability, interaction with other transcription regulators, and its ability to form a tetramer. Surprisingly, in mouse models, the activation of the best-studied p53 target genes encoding the inhibitor of the cell cycle (CDKN1A) or the inducers of apoptosis (e.g. NOXA, PUMA) is dispensable for protection against cancers. Thus, the non-classical functions of p53 must be studied to better understand its tumor suppressive mechanisms.

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Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation

Cited by 6 publications

References 42 publications

RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration

RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration

Host Immune Response Modulation in Avian Coronavirus Infection: Tracheal Transcriptome Profiling In Vitro and In Vivo

The p53 protein – not only the guardian of the genome

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