Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Nicotiana tabacum L.

Khan, Rayyan; Zhou, Peilu; Zhou, Lei; Wu, Yuanhua; Ullah, Zia; Wang, Shusheng

doi:10.3390/genes10121041

Cited by 30 publications

(20 citation statements)

References 99 publications

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“…The water stress time duration for drought-hardening ranges from a few days [20,21] to weeks [19,22] in various plant species. In our previous study Khan et al [23], drought stress for 2 or 3 days exhibited deleterious effects on plants which showed yellowing and wilting of leaves. On this basis, that time point durations were used for droughthardening in this study.…”

Section: Introductionmentioning

confidence: 86%

Drought-hardening improves drought tolerance in Nicotiana tabacum at physiological, biochemical, and molecular levels

Khan

Shah

et al. 2020

BMC Plant Biol

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Background Drought stress is the most harmful one among other abiotic stresses with negative impacts on crop growth and development. Drought-hardening is a feasible and widely used method in tobacco seedlings cultivation. It has gained extensive interests due to its role in improving drought tolerance. This research aimed to investigate the role of drought-hardening and to unravel the multiple mechanisms underlying tobacco drought tolerance and adaptation. Results This study was designed in which various drought-hardening treatments (CK (no drought-hardening), T1 (drought-hardening for 24 h), T2 (drought-hardening for 48 h), and T3 (drought-hardening for 72 h)) were applied to two tobacco varieties namely HongHuaDaJinYuan (H) and Yun Yan-100 (Y). The findings presented a complete framework of drought-hardening effect at physiological, biochemical, and gene expression levels of the two tobacco varieties under drought stress. The results showed that T2 and T3 significantly reduced the growth of the two varieties under drought stress. Similarly, among the various drought-hardening treatments, T3 improved both the enzymatic (POD, CAT, APX) and non-enzymatic (AsA) defense systems along with the elevated levels of proline and soluble sugar to mitigate the negative effects of oxidative damage and bringing osmoregulation in tobacco plants. Finally, the various drought-hardening treatments (T1, T2, and T3) showed differential regulation of genes expressed in the two varieties, while, particularly T3 drought-hardening treatment-induced drought tolerance via the expression of various stress-responsive genes by triggering the biosynthesis pathways of proline (P5CS1), polyamines (ADC2), ABA-dependent (SnRK2, AREB1), and independent pathways (DREB2B), and antioxidant defense-related genes (CAT, APX1, GR2) in response to drought stress. Conclusions Drought-hardening made significant contributions to drought tolerance and adaptation in two tobacco variety seedlings by reducing its growth and, on the other hand, by activating various defense mechanisms at biochemical and molecular levels. The findings of the study pointed out that drought-hardening is a fruitful strategy for conferring drought tolerance and adaptations in tobacco. It will be served as a useful method in the future to understand the drought tolerance and adaptation mechanisms of other plant species. Graphical abstract Drought-hardening improved drought tolerance and adaptation of the two tobacco varieties. T1 indicates drought-hardening for 24 h, T2 indicates drought-hardening for 48 h, T3 indicates drought-hardening for 72 h

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Section: Introductionmentioning

confidence: 86%

Drought-hardening improves drought tolerance in Nicotiana tabacum at physiological, biochemical, and molecular levels

Khan

Shah

et al. 2020

BMC Plant Biol

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“…The early expression of MYB, MYB-related, AP2-EREBP, NAC, HSF, and WRKY TF families in Pvgstu3–3.4 plants even in stress-free conditions and their increase under DT stress-indicate that the Pvgstu3–3.4 transgenic plants were in a primed condition prior to the induction DT stress. The MYB TFs are known to be involved in the transcriptional control of physiological and biochemical processes, including plant development, cell fate determination, and secondary metabolism along with mediating abiotic stress responses [ 78 , 80 ], such as during heat stress in eggplant [ 81 ] or combined heat and drought stress in soybean [ 82 ]. Ethylene-responsive element-binding protein (AP2-EREBP) TF family is involved in the biotic and abiotic stress response via the Jasmonic acid pathway and ABA biosynthetic pathway, respectively [ 83 ] and have been shown to be involved in early-stage drought stress responses [ 84 , 85 , 86 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought

Stavridou

Voulgari

Michailidis

et al. 2021

IJMS

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Drought and heat stresses are major factors limiting crop growth and productivity, and their effect is more devastating when occurring concurrently. Plant glutathione transferases (GSTs) are differentially expressed in response to different stimuli, conferring tolerance to a wide range of abiotic stresses. GSTs from drought-tolerant Phaseolus vulgaris var. “Plake Megalosperma Prespon” is expected to play an important role in the response mechanisms to combined and single heat and drought stresses. Herein, we examined wild-type N. tabacum plants (cv. Basmas Xanthi) and T1 transgenic lines overexpressing the stress-induced Pvgstu3–3 and Pvgstu2–2 genes. The overexpression of Pvgstu3–3 contributed to potential thermotolerance and greater plant performance under combined stress. Significant alterations in the primary metabolism were observed in the transgenic plants between combined stress and stress-free conditions. Stress-responsive differentially expressed genes (DEGs) and transcription factors (TFs) related to photosynthesis, signal transduction, starch and sucrose metabolism, osmotic adjustment and thermotolerance, were identified under combined stress. In contrast, induction of certain DEGs and TF families under stress-free conditions indicated that transgenic plants were in a primed state. The overexpression of the Pvgstu3–3 is playing a leading role in the production of signaling molecules, induction of specific metabolites and activation of the protective mechanisms for enhanced protection against combined abiotic stresses in tobacco.

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“…Based on KEGG pathway enrichment analysis, starch and sucrose metabolism pathway was identified in the MM.darkred module. The starch and sucrose metabolic process is widely identified in many plants under drought stress (Min et al, 2016;Ma et al, 2017;Khan et al, 2019;Hong et al, 2020). Trehalose, glucose, and sucrose are important soluble sugars to maintain cell osmotic potential (Konigshofer and Loppert, 2015;Han et al, 2016;Cui et al, 2019).…”

Section: Analysis Of Starch and Sucrose Metabolism In Response To Drought Stressmentioning

confidence: 99%

Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)

Zhao

Cui

et al. 2021

Front. Genet.

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Drought is one of the major abiotic stress factors limiting peanut production. It causes the loss of pod yield during the pod formation stage. Here, one previously identified drought-tolerant cultivar, “L422” of peanut, was stressed by drought (35 ± 5%) at pod formation stage for 5, 7, and 9 days. To analyze the drought effects on peanut, we conducted physiological and transcriptome analysis in leaves under well-watered (CK1, CK2, and CK3) and drought-stress conditions (T1, T2, and T3). By transcriptome analysis, 3,586, 6,730, and 8,054 differentially expressed genes (DEGs) were identified in “L422” at 5 days (CK1 vs T1), 7 days (CK2 vs T2), and 9 days (CK3 vs T3) of drought stress, respectively, and 2,846 genes were common DEGs among the three-time points. Furthermore, the result of weighted gene co-expression network analysis (WGCNA) revealed one significant module that was closely correlated between drought stress and physiological data. A total of 1,313 significantly up-/down-regulated genes, including 61 transcription factors, were identified in the module at three-time points throughout the drought stress stage. Additionally, six vital metabolic pathways, namely, “MAPK signaling pathway-plant,” “flavonoid biosynthesis,” “starch and sucrose metabolism,” “phenylpropanoid biosynthesis,” “glutathione metabolism,” and “plant hormone signal transduction” were enriched in “L422” under severe drought stress. Nine genes responding to drought tolerance were selected for quantitative real-time PCR (qRT-PCR) verification and the results agreed with transcriptional profile data, which reveals the reliability and accuracy of transcriptome data. Taken together, these findings could lead to a better understanding of drought tolerance and facilitate the breeding of drought-resistant peanut cultivars.

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Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Nicotiana tabacum L.

Cited by 30 publications

References 99 publications

Drought-hardening improves drought tolerance in Nicotiana tabacum at physiological, biochemical, and molecular levels

Drought-hardening improves drought tolerance in Nicotiana tabacum at physiological, biochemical, and molecular levels

Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought

Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)

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