Transcriptome profiling of anti-müllerian hormone treated preantral/small antral mouse ovary follicles

Abstract

The predisposition for the initiation of folliculogenesis in mammals including humans is programmed to start at fetal life and continues until reproductive capacity. The follicles grow from a pool of primordial follicles which retain the major functions in the entire reproductive life of a female. Anti-müllerian hormone (AMH), a glycoprotein belonging to the transforming growth factor-beta family, has an inhibitory effect on ovarian follicle development. The key regulatory target genes in primordial follicle development are of paramount importance in reproductive biology of female. A systems biology method was used to find regulatory genes performing critical role in primordial follicle development. A complete in-depth bioinformatics analysis was performed to investigate the changes in transcriptome of preantral to small antral mouse follicles treated for 12 h and 24 h with two different concentrations; 50 and 200 ng/ml of AMH, and thereby identify candidate genes in time and concentration manner. Firstly, we found differentially expressed genes that were time and concentration dependent in response to AMH. The network analysis of these differentially expressed genes provided new candidate genes and pathways associated with inhibitory action of AMH on the primordial follicle development. To further emphasize the function of AMH, the key identified genes’ protein-protein docking was analyzed and found the intracellular and extracellular protein-protein interaction. This study elucidates one of the novel mechanisms of AMH involvement in inhibition of ovarian follicle development which may lead to prolong productive life in female.

Anti-Mullerian hormone attenuates both cyclophosphamide-induced damage and PI3K signalling activation, while rapamycin attenuates only PI3K signalling activation, in human ovarian cortex in vitro

“…AMH receptor 2 expression has also been reported in these cell types but not in oocytes ( Weenen et al , 2004 ; Meinsohn et al , 2021 ), suggesting the chemoprotective effect of AMH on non-growing follicle oocytes may be indirect, and could potentially involve maintaining or stabilizing the oocyte–somatic cell interactions. Furthermore, the effect of AMH is likely to be dose-dependent, with a separate transcriptomic study showing an AMH dose-related and time-dependent response ( Ur Rehman et al , 2018 ). Nevertheless, our knowledge is lacking on the effects of AMH on cell-to-cell communication and extracellular matrix composition, both important variables that can dictate the physiological state of ovaries ( Fraire-Zamora et al , 2023 ).…”