Transcriptome profiling of pepper leaves by RNA-Seq during an incompatible and a compatible pepper-tobamovirus interaction

Kalapos, Balázs; Juhász, Csilla; Balogh, Eszter; Kocsy, Gábor; Tóbiás, István; Gullner, Gábor

doi:10.1038/s41598-021-00002-5

Cited by 13 publications

(16 citation statements)

References 127 publications

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“…Interestingly, transcriptomic analyses revealed that the GST expression profile can be differentially regulated in plant–virus interactions, such as pepper leaves infected with Obuda pepper virus [ 53 ], Rice stripe virus disease in Arabidopsis [ 54 ], Beta vulgaris – Beet necrotic yellow vein virus (BNYVV) interactions [ 55 ], and response of watermelon to Cucumber green mottle mosaic virus [ 56 ]. Moreover, in the response of susceptible A. thaliana to Cauliflower mosaic virus , systemic induction of GST1 with increased virus titers and development of symptoms was observed [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

AtGSTU19 and AtGSTU24 as Moderators of the Response of Arabidopsis thaliana to Turnip mosaic virus

Otulak

Kozieł

Horváth

et al. 2022

IJMS

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Plants produce glutathione as a response to the intercellular redox state. Glutathione actively participates in the reactive oxygen species (ROS)-dependent signaling pathway, especially under biotic stress conditions. Most of the glutathione S-transferases (GSTs) are induced in cells during the defense response of plants not only through highly specific glutathione-binding abilities but also by participating in the signaling function. The tau class of GSTs has been reported to be induced as a response under stress conditions. Although several studies have focused on the role of the tau class of GSTs in plant–pathogen interactions, knowledge about their contribution to the response to virus inoculation is still inadequate. Therefore, in this study, the response of Atgstu19 and Atgstu24 knockout mutants to mechanical inoculation of Turnip mosaic virus (TuMV) was examined. The systemic infection of TuMV was more dynamically promoted in Atgstu19 mutants than in wild-type (Col-0) plants, suggesting the role of GSTU19 in TuMV resistance. However, Atgstu24 mutants displayed virus limitation and downregulation of the relative expression of TuMV capsid protein, accompanied rarely by TuMV particles only in vacuoles, and ultrastructural analyses of inoculated leaves revealed the lack of virus cytoplasmic inclusions. These findings indicated that Atgstu24 mutants displayed a resistance-like reaction to TuMV, suggesting that GSTU24 may suppress the plant resistance. In addition, these findings confirmed that GSTU1 and GSTU24 are induced and contribute to the susceptible reaction to TuMV in the Atgstu19–TuMV interaction. However, the upregulation of GSTU19 and GSTU13 highly correlated with virus limitation in the resistance-like reaction in the Atgstu24–TuMV interaction. Furthermore, the highly dynamic upregulation of GST and glutathione reductase (GR) activities resulted in significant induction (between 1 and 14 days post inoculation [dpi]) of the total glutathione pool (GSH + GSSG) in response to TuMV, which was accompanied by the distribution of active glutathione in plant cells. On the contrary, in Atgstu19, which is susceptible to TuMV interaction, upregulation of GST and GR activity only up to 7 dpi symptom development was reported, which resulted in the induction of the total glutathione pool between 1 and 3 dpi. These observations indicated that GSTU19 and GSTU24 are important factors in modulating the response to TuMV in Arabidopsis thaliana. Moreover, it was clear that glutathione is an important component of the regulatory network in resistance and susceptible response of A. thaliana to TuMV. These results help achieve a better understanding of the mechanisms regulating the Arabidopsis–TuMV pathosystem.

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Section: Discussionmentioning

confidence: 99%

AtGSTU19 and AtGSTU24 as Moderators of the Response of Arabidopsis thaliana to Turnip mosaic virus

Otulak

Kozieł

Horváth

et al. 2022

IJMS

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“…Plant GSTs (EC 2.5.1.18) are essential enzymes involved in diverse functions, such as detoxification, redox homeostasis, plant metabolism, signaling, and especially regulation and adaptation to abiotic and biotic stress [ 25 ]. Transcriptome profiling of pepper leaves in compatible and incompatible pepper tobamovirus interactions revealed that infection by Obuda pepper virus (ObPV) strongly induced GST genes [ 63 ]. The inoculation of ObPV resulted in the activation of cysteine and GSH biosynthesis pathway.…”

Section: Discussionmentioning

confidence: 99%

“…The inoculation of ObPV resulted in the activation of cysteine and GSH biosynthesis pathway. Kalapos et al [ 63 ] indicated that 22 GSTs were highly induced at 3 dpi. Genome wide analyses of GST genes have been performed in various plants, and the results revealed the presence of 55 genes in Arabidopsis [ 64 ] and a total of 90 genes in S. tuberosum [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Glutathione Modulation in PVYNTN Susceptible and Resistant Potato Plant Interactions

Otulak

Kozieł

Przewodowski

et al. 2022

IJMS

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Glutathione is a metabolite that plays an important role in plant response to biotic stress through its ability to remove reactive oxygen species, thereby limiting the degree of potential oxidative damage. It can couple changes in the intracellular redox state to the development, especially the defense responses, of plants. Several studies have focused on measuring glutathione levels in virus infected plants, but have not provided complete information. Therefore, we analyzed, for the first time, the content of glutathione as well as its ultrastructural distribution related to susceptible and hypersensitive potato–Potato virus Y NTN (PVYNTN) interaction, with an aim of providing new insight into interactive responses to PVYNTN stress. Our findings reported that the inoculation of PVYNTN caused a dynamic increase in the content of glutathione, not only in resistance but also in susceptible reaction, especially at the first steps of plant–virus interaction. Moreover, the increase in hypersensitive response was much more dynamic, and accompanied by a significant reduction in the content of PVYNTN. By contrast, in susceptible potato Irys, the content of glutathione decreased between 7 and 21 days after virus inoculation, which led to a significant increase in PVYNTN concentration. Additionally, our findings clearly indicated the steady induction of two selected potato glutathione S-transferase StGSTF1 and StGSTF2 genes after PVYNTN inoculation, regardless of the interaction type. However, the relative expression level of StGSTF1 did not significantly differ between resistant and susceptible plants, whereas the relative expression levels of StGSTF2 differed between susceptible and resistant reactions. Therefore, we proposed that StGSTF2 can act as a marker of the type of response to PVYNTN. Our observations indicated that glutathione is an important component of signaling as well as the regulatory network in the PVYNTN–potato pathosystem. In resistance responses to PVYNTN, this metabolite activates plant defenses by reducing potential damage to the host plant cell, causing a reduction in virus concentration, while it can also be involved in the development of PVYNTN elicited symptoms, as well as limiting oxidative stress, leading to systemic infection in susceptible potato plants.

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“…Ensembl identifiers and transcript sequences of differentially expressed Vitis vinifera genes were used to collect functional information in KEGG, PFAM, GO, and TAIR databases as recently described [ 96 ]. Protein-protein interactions were predicted by the STRING v11 database.…”

Section: Methodsmentioning

confidence: 99%

Redox and Hormonal Changes in the Transcriptome of Grape (Vitis vinifera) Berries during Natural Noble Rot Development

Pogány

Dankó

Hegyi-Kaló

et al. 2022

Plants

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Noble rot is a favorable form of the interaction between grape (Vitis spp.) berries and the phytopathogenic fungus Botrytis cinerea. The transcriptome pattern of grapevine cells subject to natural noble rot development in the historic Hungarian Tokaj wine region has not been previously published. Furmint, a traditional white Tokaj variety suited to develop great quality noble rot was used in the experiments. Exploring a subset of the Furmint transcriptome redox and hormonal changes distinguishing between noble rot and bunch rot was revealed. Noble rot is defined by an early spike in abscisic acid (ABA) accumulation and a pronounced remodeling of ABA-related gene expression. Transcription of glutathione S-transferase isoforms is uniquely upregulated, whereas gene expression of some sectors of the antioxidative apparatus (e.g., catalases, carotenoid biosynthesis) is downregulated. These mRNA responses are lacking in berries exposed to bunch rot. Our results help to explain molecular details behind the fine and dynamic balance between noble rot and bunch rot development.

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Transcriptome profiling of pepper leaves by RNA-Seq during an incompatible and a compatible pepper-tobamovirus interaction

Cited by 13 publications

References 127 publications

AtGSTU19 and AtGSTU24 as Moderators of the Response of Arabidopsis thaliana to Turnip mosaic virus

AtGSTU19 and AtGSTU24 as Moderators of the Response of Arabidopsis thaliana to Turnip mosaic virus

Glutathione Modulation in PVYNTN Susceptible and Resistant Potato Plant Interactions

Redox and Hormonal Changes in the Transcriptome of Grape (Vitis vinifera) Berries during Natural Noble Rot Development

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