Transcriptome Profiling Reveals Molecular Changes during Flower Development between Male Sterile and Fertile Chinese Cabbage (Brassica rapa ssp. pekinensis) Lines

Abstract

Male sterility exists widely in flowering plants and is used as a fascinating tool by breeders for creating hybrid varieties. Herein, stamen samples from male sterile CCR20000 and male fertile CCR20001 lines during two developmental stages were employed to elucidate the molecular changes during flower development in fertile and sterile Chinese cabbage lines. RNA-seq revealed weak transcriptional activity in the sterile line, which may have led to the abnormal stamen development. The differentially expressed genes were enriched in plant hormone, carbon metabolism, and biosynthesis of amino acid pathways. Important genes with opposite patterns of regulation between the two lines have been associated with the male sterility trait. Members of the transcription factor families such as AP2, MYB, bHLH, and WRKY were highly active in the regulation of structural genes involved in pollen fertility. This study generated important genomic information to support the exploitation of the male sterility trait in Chinese cabbage breeding programs.

Whole-transcriptome analyses identify key differentially expressed mRNAs, lncRNAs, and miRNAs associated with male sterility in watermelon

“…Beyond protein-coding genes, non-coding RNAs (ncRNAs) including microRNAs (miRNAs), small interfering RNAs (siRNAs), circular RNAs (circRNAs), and long non-coding RNAs (lncRNAs), have also been confirmed to participate in various aspects of plant growth and development, such as stress-related responses (Hu et al, 2021;Ren et al, 2021;Shi et al, 2021;Wang et al, 2021) and male reproductive development (Li et al, 2015;Yuan et al, 2020). As endogenous non-coding RNAs of 21-25 nt in length, miRNAs mainly contribute to post-transcriptional regulation of downstream targets through mRNA cleavage.…”

Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)

“…Male sterility (MS) refers to the inability to produce functional anthers or pollens, which plays an important role in developing high yielding and vigorous cultivars as an economical and effective system for pollination control [ 1 ]. Based on the inheritance, MS is classified into cytoplasmic male sterility (CMS) and genetic male sterility (GMS) [ 2 ].…”